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Comparative reevaluation of FASP and enhanced FASP methods by LC-MS/MS


, : Comparative reevaluation of FASP and enhanced FASP methods by LC-MS/MS. Journal of Proteome Research 14(3): 1637-1642

Filter-aided sample preparation is a proteomic technique for the preparation and on column proteolysis of proteins. Recently an enhanced FASP protocol was developed that uses deoxycholic acid (DCA) and that reportedly enhances trypsin proteolysis, resulting in increases cytosolic and membrane protein representation. FASP and eFASP were re-evaluated by ultra-high-performance liquid chromatography coupled to a quadrupole mass filter Orbitrap analyzer (Q Exactive). Although there was no difference in trypsin activity, 14,099 and 13,414 peptides, describing 1723 and 1793 protein groups, from Escherichia coli K12 were identified using FASP and eFASP, respectively. Characterization of the physicochemical properties of identified peptides showed no significant differences other than eFASP extracting slightly more basic peptides. At the protein level, both methods extracted essentially the same number of hydrophobic transmembrane helix-containing proteins as well as proteins associated with the cytoplasm or the cytoplasmic and outer membranes. By employing state-of-the-art LC-MS/MS shot gun proteomics, our results indicate that FASP and eFASP showed no significant differences at the protein level. However, because of the slight differences in selectivity at the physicochemical level of peptides, these methods can be seen to be somewhat complementary for analyses of complex peptide mixtures.

US$19.90

PMID: 25619111

DOI: 10.1021/pr501266c


Other references

Bertschinger, H.U.; Bürgi, E.; Eng, V.; Wegmann, P., 1990: Lowering of the incidence of puerperal mastitis in the sow by protection of the mammae from contamination. Twelve sows each farrowed in an experimental pen designed especially for this study or in a crate. Viable counts of enterobacteriaceae were performed in samples taken from the laying area and from the teats. Secretion from every mammary complex wa...

Crawfurd, R., 1931: The Place Of Medical Societies In The Progress Of Medicine. British Medical Journal 1(3675): 1036-1040

Qurishi, N.; Kreuzberg, C.; Voigt, E.; Hess, L.; Effenberger, W.; Luechters, G.; Kupfer, B.; Sauerbruch, T.; Rockstroh, K.J.ergen; Spengler, U., 2002: Effect of anti-retroviral therapy on liver-related mortality in HIV/HCV coinfected patients. Journal of Hepatology 36(Supplement 1): 120, April

Volgin P.F.; Parovyshnyy V.A.; Radyush V.M., 1998: Overthrust zones as new oil-gas exploration targets in south Sakhalin. Petroleum Geology, a Quarterly Journal 32(4): 402-407

Wade, M.; Tucker, I.; Cunningham, P.; Skinner, R.; Bell, F.; Lyons, T.; Patten, K.; Gonzalez, L.; Wess, T., 2014: Investigating the origins of nanostructural variations in differential ethnic hair types using X-ray scattering techniques. Human hair is a major determinant of visual ethnic differentiation. Although hair types are celebrated as part of our ethnic diversity, the approach to hair care has made the assumption that hair types are structurally and chemically similar. Alth...

Dohr Gerhard; Fuchs Karl, 1967: Statistical evaluation of deep crustal reflections in germany. By a routine method of observation during exploration reflection measurements, sufficient data were compiled to allow significant statistical conclusions concerning deep crustal reflectors. The appearance of deep crustal reflections differs consid...

Cole, J.B.; Moore, J.C.; Dolan, B.J.; O'Brien-Lambert, A.; Fryza, B.J.; Miner, J.R.; Martel, M.L., 2016: A Prospective Observational Study of Patients Receiving Intravenous and Intramuscular Olanzapine in the Emergency Department. Parenteral olanzapine is an emerging therapy for a variety of conditions in the emergency department (ED). Intramuscular administration is standard; however, intravenous administration has been proposed as a safe alternative route. We investigate...

Rajagopal, S.N.; Sandine, W.E.; Ayres, J.W., 1990: Whey-based bacteriophage inhibitory Italian bulk starter medium. Journal of Dairy Science 73(4): 881-886

MacCallum, D.K.; Bahn, C.F.; Lillie, J.H.; Meyer, R.F.; Martonyi, C.L., 1983: Evidence for corneal endothelial cell hypertrophy during postnatal growth of the cat cornea. Endothelial cell counts made from specular micrographs of the cornea of one-month-old kittens and adult cats revealed a progressive increase in endothelial cell size and a reduction in endothelial cell density during postnatal development. Kitten...

Rivallan, A., 2016: Not Available. Soins. Psychiatrie 37(305): 11-40