geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Concentration-dependent Effects of Nuclear Lamins on Nuclear Size in Xenopus and Mammalian Cells


, : Concentration-dependent Effects of Nuclear Lamins on Nuclear Size in Xenopus and Mammalian Cells. Journal of Biological Chemistry 290(46): 27557-27571

A fundamental question in cell biology concerns the regulation of organelle size. While nuclear size is exquisitely controlled in different cell types, inappropriate nuclear enlargement is used to diagnose and stage cancer. Clarifying the functional significance of nuclear size necessitates an understanding of the mechanisms and proteins that control nuclear size. One structural component implicated in the regulation of nuclear morphology is the nuclear lamina, a meshwork of intermediate lamin filaments that lines the inner nuclear membrane. However, there has not been a systematic investigation of how the level and type of lamin expression influences nuclear size, in part due to difficulties in precisely controlling lamin expression levels in vivo. In this study, we circumvent this limitation by studying nuclei in Xenopus laevis egg and embryo extracts, open biochemical systems that allow for precise manipulation of lamin levels by the addition of recombinant proteins. We find that nuclear growth and size are sensitive to the levels of nuclear lamins, with low and high concentrations increasing and decreasing nuclear size, respectively. Interestingly, each type of lamin that we tested (lamins B1, B2, B3, and A) similarly affected nuclear size whether added alone or in combination, suggesting that total lamin concentration, and not lamin type, is more critical to determining nuclear size. Furthermore, we show that altering lamin levels in vivo, both in Xenopus embryos and mammalian tissue culture cells, also impacts nuclear size. These results have implications for normal development and carcinogenesis where both nuclear size and lamin expression levels change.

US$19.90

PMID: 26429910

DOI: 10.1074/jbc.M115.673798


Other references

Miller, Ja, 1987: The sandwich generation. Working mother 10(1): 46-48

Stankovich, J.M.; Cooley, H.M.; Sale, M.M.; Bahlo, M.; Speed, T.P.; Dickinson, J.; Jones, G., 2001: Investigation of linkage at chromosome 2q in osteoarthritis of the hand. European Journal of Human Genetics 9(Supplement 1): P1292

Rodriquez, R.R.villa, M.; Perez, C.C.branes, C.T.mes, R., 1987: Callus induction and a preliminary attempt to obtain cell suspensions from embryos and leaves of Juglans regia L. Acta Horticulturae 212: 683-686

Partmann, W., 1972: Some observations on the main ATPase system of muscle impaired by freezer burn and freeze-drying. Scienza dell'alimentazione: 18 (9) 283-287

Cowan, S.; Young, G.B., 1996: Improving prescribing through use of CPS. Cmaj 155(5): 512-513

Dement'ev, E.Z.; Ivanov, E.D.; Melent'eva, E.G.; Chkhikvadze, V.D., 1979: Dynamic x-ray observations of pulmonary aspergilloma. Vestnik Rentgenologii i Radiologii: 38-44

Altschule M.D., 1968: Studies on aminochromes iii transformation of epinephrine adrenochrome and adrenolutin into plasma soluble melanins during incubation in human blood plasma inst paper chromatography. Archives Of Biochemistry & Biophysics: 388-392

Jaeger Fritz, 1933: Lime pans in eastern South-West Africa. Pan-American Geologist 60(4): 316-317

Bamann, E.; Oechsner, B.; Trapmann, H., 1961: The effect of metal ions on the phosphate-containing moiety of thrombokinase. Hoppe-Seyler's Zeitschrift für Physiologische Chemie 323: 77-87

Montero-De-Burgos, Jl, 1981: Coordination of actions implicated in reforestation in order to obtain maximum efficiency and total economy in the works. XVII IUFRO World Congress Japan 1981 International Union of Forest Research Organizations = Internationaler Verband Forstlicher Forschungsanstalten = Union internationale des instituts des recherches forestieres: 267