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Conjugated fatty acids and methane production by rumen microbes when incubated with linseed oil alone or mixed with fish oil and/or malate

, : Conjugated fatty acids and methane production by rumen microbes when incubated with linseed oil alone or mixed with fish oil and/or malate. Animal Science Journal 86(8): 755-764

We hypothesized that manipulating metabolism with fish oil and malate as a hydrogen acceptor would affect the biohydrogenation process of α-linolenic acid by rumen microbes. This study was to examine the effect of fish oil and/or malate on the production of conjugated fatty acids and methane (CH4 ) by rumen microbes when incubated with linseed oil. Linseed oil (LO), LO with fish oil (LO-FO), LO with malate (LO-MA), or LO with fish oil and malate (LO-FO-MA) was added to diluted rumen fluid, respectively. The LO-MA and LO-FO-MA increased pH and propionate concentration compared to the other treatments. LO-MA and LO-FO-MA reduced CH4 production compared to LO. LO-MA and LO-FO-MA increased the contents of c9,t11-conjugated linoleic acid (CLA) and c9,t11,c15-conjugated linolenic acid (CLnA) compared to LO. The content of malate was rapidly reduced while that of lactate was reduced in LO-MA and LO-FO-MA from 3 h incubation time. The fold change of the quantity of methanogen related to total bacteria was decreased at both 3 h and 6 h incubation times in all treatments compared to the control. Overall data indicate that supplementation of combined malate and/or fish oil when incubated with linseed oil, could depress methane generation and increase production of propionate, CLA and CLnA under the conditions of the current in vitro study.


PMID: 25597643

DOI: 10.1111/asj.12354

Other references

Alt, F.; Weber, G.; Messerschmidt, J.; Von Bohlen, A.; Kastenholz, B.; Guenther, K., 2002: Bonding states of palladium in phytosystems: First results for endive. The binding of palladium to organic ligands was studied in leaves from contaminated endive. The palladium-treated plants contained 8.7 ng Pd/g referring to wet weight. After a cell breakdown in buffer the resulting homogenate was separated into su...

Riabchenko, N.I.; Ivannik, B.P., 1982: Analysis of the damage, repair and degradation of DNA in tissues of irradiated animals. Meditsinskaia Radiologiia 27(9): 21-25

Savel'ev, V.S.; Usvatova, I.Ia.; Ognev, I.V.; Mitronov, I.A., 1973: Changes in the kinin system of blood in acute pancreatitis. Vestnik Khirurgii Imeni I. I. Grekova 111(8): 46-51

Burland, CB., 1991: Great white egret - a new Isle of Wight species. Isle of Wight Birds, 1990: 64-65

Asaumi, S.; Mori, S.; Takemoto, M.; Yokote, K.; Saito, Y., 2000: Enhanced expression of osteopontin by high glucose Involvement of osteopontin in diabetic vascular disease. Journal of Submicroscopic Cytology & Pathology 32(3): 473, July

Chen, C-Chung.; Tyler, C.W., 2006: Evidence for elongated receptive field structure for mechanisms subserving stereopsis. To study the spatial extent and shape of the binocular disparity mechanisms subserving depth perception. we employ the spatial summation paradigm of contrast threshold for front/back depth discrimination at a fixed binocular disparity. The stimuli...

Shu, L.; Li, C.; Zhang, X., 2016: The role of shrimp miR-965 in virus infection. RNAi, mediated by microRNAs (miRNAs), has attracted increasing attention for its important role in cross-talk between host and virus. However, the role of host miRNA in the virus infection in vivo has not been intensively investigated. In this st...

Ramacciotti, F.E., 1959: The surface fixation test in brucellosis. Revista de la Facultad de Ciencias Medicas de Cordoba 17: 289-296

Bacala, J.C., 1954: Blood donor fatality; due to axillary vein thrombosis and pulmonary embolism. Missouri Medicine 51(6): 462-463

Vovenko, E.P., 2009: Transmural oxygen tension gradients in rat cerebral cortex arterioles. Modified needle oxygen microelectrodes and vital microscopy were used to measure transmural oxygen tension gradients (PO2) in pial arterioles with lumen diameters of 20-90 microm. A relationship between the magnitude of the transmural PO2 gradient...