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Constitutive Notch Signaling Causes Abnormal Development of the Oviducts, Abnormal Angiogenesis, and Cyst Formation in Mouse Female Reproductive Tract


, : Constitutive Notch Signaling Causes Abnormal Development of the Oviducts, Abnormal Angiogenesis, and Cyst Formation in Mouse Female Reproductive Tract. Biology of Reproduction 94(3): 67-67

The Notch signaling pathway is critical for the differentiation of many tissues and organs in the embryo. To study the consequences of Notch1 gain-of-function signaling on female reproductive tract development, we used a cre-loxP strategy and Amhr2-cre transgene to generate mice with conditionally activated Notch1 (Rosa(Notch1)). The Amhr2-cre transgene is expressed in the mesenchyme of developing female reproductive tract and in granulosa cells in the ovary. Double transgenic Amhr2-cre, Rosa(Notch1) females were infertile, whereas control Rosa(Notch1) mice had normal fertility. All female reproductive organs in mutants showed hemorrhaging of blood vessels progressing with age. The mutant oviducts did not develop coiling, and were instead looped around the ovary. There were multiple blockages in the lumen along the oviduct length, creating a barrier for sperm or oocyte passage. Mutant females demonstrated inflamed uteri with increased vascularization and an influx of inflammatory cells. Additionally, older females developed ovarian, oviductal, and uterine cysts. The significant change in gene expression was detected in the mutant oviduct expression of Wnt4, essential for female reproductive tract development. Similar oviductal phenotypes have been detected previously in mice with activated Smo and in beta-catenin, Wnt4, Wnt7a, and Dicer conditional knockouts, indicating a common regulatory pathway disrupted by these genetic abnormalities.

US$19.90

PMID: 26843448

DOI: 10.1095/biolreprod.115.134569


Other references

Ulrich, H.D., 2002: Degradation or maintenance: actions of the ubiquitin system on eukaryotic chromatin. Eukaryotic Cell 1(1): 1-10

Johnson, K., 1989: The hairstreak butterfly genus Noreena (Lycaenidae, Theclinae) and a new species from western Peru. Insecta Mundi, 33: 247-248

Berardo, D.; Shehan, C.; Leslie, G., 1987: A residue of tradition: jobs, careers, and spouses time in housework. Journal of marriage and the family 49(2): 381-390

Low, J.G.H.; Lee, L.S.; Ooi, E.Eong.; Ethirajulu, K.; Yeo, P.; Matter, A.; Connolly, J.E.; Skibinski, D.A.G.; Saudan, P.; Bachmann, M.; Hanson, B.J.; Lu, Q.; Maurer-Stroh, S.; Lim, S.; Novotny-Diermayr, V., 2015: Safety and immunogenicity of a virus-like particle pandemic influenza A (H1N1) 2009 vaccine: results from a double-blinded, randomized Phase I clinical trial in healthy Asian volunteers. A novel, fully bacterially produced recombinant virus-like particle (VLP) based influenza vaccine (gH1-Qbeta) against A/California/07/2009(H1N1) was tested in a double-blind, randomized phase I clinical trial at two clinical sites in Singapore. Th...

Yao, G-zhong.; Zeng, L-qiang., 2003: Report on six cases of paragonimiasis. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 20(4): 228-228

Marcus P.I.; Sekellick M.J., 1984: Interferon induction suppressing particles. Antiviral Research 4(SPEC ISSUE): 140

Anonymous, 1926: Public Health Weekly Reports for JULY 16, 1926. Public Health Reports 41(29): 1463-1510

Forrester, T., 2004: Historic and early life origins of hypertension in Africans. Cardiovascular disease (CVD) causes 12.4 million deaths annually, most (9.6 million) occurring in developing countries. Hypertension, the most common CVD, arises within the context of obesity, but the underlying mechanisms remain obscure. Obesity...

Martinotti, M.; Vassallo, C., 1990: Alloplasts in the therapy of hernias. With the use of mesh prosthetics, it is now easy to repair most difficult hernias with no suture line tension. The Authors report their experience (1984-1988) of dacron meshes: they performed 63 alloplasties with a total of 665 repairs for hernias...

Altomani, L., 1979: Internal and external factors which modify the innate behaviour of the mouse in the double Y maze. A test to study an innate behaviour of mice was standardized and the factors which could modify the results were examined. A track left by a mouse had effect upon the choices of the next one, the homogeneity of light at the exits was important, wh...