geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Design and Construction of Artificial Extracellular Matrix (aECM) Proteins from Escherichia coli for Skin Tissue Engineering


, : Design and Construction of Artificial Extracellular Matrix (aECM) Proteins from Escherichia coli for Skin Tissue Engineering. Journal of Visualized Experiments 100): E52845-E52845

Recombinant technology is a versatile platform to create novel artificial proteins with tunable properties. For the last decade, many artificial proteins that have incorporated functional domains derived from nature (or created de novo) have been reported. In particular, artificial extracellular matrix (aECM) proteins have been developed; these aECM proteins consist of biological domains taken from fibronectin, laminins and collagens and are combined with structural domains including elastin-like repeats, silk and collagen repeats. To date, aECM proteins have been widely investigated for applications in tissue engineering and wound repair. Recently, Tjin and coworkers developed integrin-specific aECM proteins designed for promoting human skin keratinocyte attachment and propagation. In their work, the aECM proteins incorporate cell binding domains taken from fibronectin, laminin-5 and collagen IV, as well as flanking elastin-like repeats. They demonstrated that the aECM proteins developed in their work were promising candidates for use as substrates in artificial skin. Here, we outline the design and construction of such aECM proteins as well as their purification process using the thermo-responsive characteristics of elastin.

US$19.90

PMID: 26132812

DOI: 10.3791/52845


Other references

Arıkan-Ayyıldız, Z.; Yuce, A.; Uslu-Kızılkan, N.; Demir, Hülya.; Gurakan, F., 2011: Immunoglobulin abnormalities and effects of enzyme replacement therapy in children with Gaucher disease. Hyperimmunoglobulinemia is documented in patients with Gaucher disease of all ages. We investigated the frequency of hyperimmunoglobulinemia in 12 pediatric patients with type I and III Gaucher disease and the effects of enzyme replacement therapy...

Zhang, S.; Huo, X.; Li, Z.; Li, X.; Tang, W.; Li, C.; Guo, M.; Du, X.; Chen, Z., 2016: Microsatellite instability detected in tumor-related genes in C57BL/6J mice with thymic lymphoma induced by N-methyl-N-nitrosourea. Microsatellite instability (MSI) has been observed within tumors and found to be closely associated with the degree of malignancy and prognosis in tumors. However, whether MSI in tumor-related genes can be induced by a chemical and whether a conne...

Anon., 1938: Underhead system for irrigating citrus trees. A method of sprinkler irrigation used by a citrus grower in California is described. The sprinklers which throw square jets (for economy) are fixed one each on 20 ft. lengths of 1 inch steel tubing connected to each other to the number of 3 and to...

Grüneberg, H., 1965: Genes and genotypes affecting the teeth of the mouse. Comparison between some inbred strains of mice revealed the existence of several inherited variations of the crowns of the 1st molars. Similar variants were found in wild mice. The inbred strains also differ characteristically in the morphology of...

Lohse, W.; Bohm, J.; Staffa, G.; Warnke, H.; Seifert, R.; Graubaum, H.J., 1988: Biochemical studies of the assessment of transplant function following clinical heart transplantation--initial experiences. Average and specific individual courses of the following parameters are reported in this paper, with reference being made to eight of eleven clinical heart transplantations so far performed at the authors' department: lactate, pyruvate, myogl...

Mokyevsky, O.B., 1967: The changes of the intertidal biota from the Bering Sea to the Sunda Strait. Okeanologiya, 2: 321-324

Lovelock, J.E., 1955: The physical instability of human red blood cells. Effects on human red cells of repeated washing and of exposure to a neutral adsorbent substance, alumina, was studied. Both of these treatments removed considerable quantities of the lipid components of the cell membrane, and caused a reduction of...

Ro, Y.S.; Jeong, S.J., 1995: Expression of the nucleoside diphosphate kinase in human skin cancers: an immunohistochemical study. Expression of nucleoside diphosphate(NDP) kinase, which is homologous to the nm23 gene product in a variety of species, has been found to be inversely associated with metastatic potential. However, the relationship remains controversial according...

Honda, Y.; Natsui, T.; Hasumura, N.; Nakamura, K., 1963: Threshold Pco2 For Respiratory System In Acute Hypoxia Of Dogs. Threshold alveolar-arterial PCO2 (PTcO2) for tne respiratory system in acute hypoxia of anesthetized dogs was examined by observing phrenic nerve discharges. It was found that 1) the lower the alveolar PO2, the lower the PTcO2. Tne relationship be...

Burrell, M.R.; Just, V.J.; Bowater, L.; Fairhurst, S.A.; Requena, L.; Lawson, D.M.; Bornemann, S., 2007: Oxalate decarboxylase and oxalate oxidase activities can be interchanged with a specificity switch of up to 282,000 by mutating an active site lid. Oxalate decarboxylases and oxalate oxidases are members of the cupin superfamily of proteins that have many common features: a manganese ion with a common ligand set, the substrate oxalate, and dioxygen (as either a unique cofactor or a substrate)...