+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Development of an infusion bioreactor for the accelerated preparation of decellularized skeletal muscle scaffolds

, : Development of an infusion bioreactor for the accelerated preparation of decellularized skeletal muscle scaffolds. Biotechnology Progress 32(3): 745-755

The implantation of decellularized tissue has shown effectiveness as a strategy for the treatment of volumetric muscle loss (VML) injuries. The preparation of decellularized tissue typically relies on the diffusion driven removal of cellular debris. For bulky tissues like muscle, the process can be lengthy, which introduces opportunities for both tissue contamination and degradation of key ECM molecules. In this study we report on the accelerated preparation of decellularized skeletal muscle (DSM) scaffolds using a infusion system and examine scaffold performance for the repair of VML injuries. The preparation of DSM scaffolds using infusion was dramatically accelerated. As the infusion rate (1% SDS) was increased from 0.1 to 1 and 10ml/hr, the time needed to remove intracellular myoglobin and actin decreased from a maximum of 140 ± 3hrs to 45 ± 3hrs and 10 ± 2hrs respectively. Although infusion appeared to remove cellular debris more aggressively, it did not significantly decrease the collagen or glycosaminoglycan composition of DSM samples when compared to un-infused controls. Infusion prepared DSM samples retained the aligned network structure and mechanical integrity of control samples. Infusion prepared DSM samples supported the attachment and in-vitro proliferation of myoblast cells and was well tolerated by the host when examined in-vivo. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:745-755, 2016.


PMID: 26949076

DOI: 10.1002/btpr.2257

Other references

Chang, H.T.; Wilson, C.J.; Kitai, S.T., 1981: Single neostriatal efferent axons in the globus pallidus: a light and electron microscopic study. Intracellularly labeled rat neostriatal projection neurons were analyzed with both light and electron microscopy. The axons of medium spiny neurons were traced into the globus pallidus and were found to make synaptic contacts with pallidal dendrit...

Vidal, J.; Fourcade, J.; Guin, J.J.; Marty, J.C., 1959: A rather peculiar anatomo-radiological aspect of the pulmonary cancer-infarct association: the aseptic cavitation of the infarct. Le Poumon et le Coeur 15: 1207-1211

Hart F.J.; Leibholz J., 1990: A note on the flow of endogenous protein to the omasum and abomasum of steers. Three steers cannulated in the rumen and abomasum were given a diet of wheat straw, chopped and soaked in sodium hydroxide and then washed to pH less than 8. The straw was sprayed with sucrose, urea and minerals. The diet was free of digestible pr...

Fujita, F.; Eguchi, S.; Takatsuki, M.; Kobayashi, K.; Kanetaka, K.; Ito, M.; Abe, K.; Kuroki, T., 2015: A recurrent granulosa cell tumor of the ovary 25 years after the initial diagnosis: A case report. Granulosa cell tumors (GCTs) are rare functional sex-cord-stromal ovarian neoplasms characterized by low malignancy potential and late relapse, which rarely metastasize to the liver. A 43-year-old female, who had undergone surgery to treat a GCT o...

Barness, L.A.; Young, L.N., 1964: A Simplified View Of Fluid Therapy. Pediatric Clinics of North America 11: 3-15

Wagner, M., 1962: Extracellular deoxyribonucleases in bacteria.. In a survey of 562 bacterial strains of 133 different species from 14 families it was found that the production of extracellular DNAase was not characteristic for any species and failed to indicate the pathogenicity of a strain, as has been postul...

Grobov, O.; Smirnov, A., 1987: Beekeeping in India. Pchelovodstvo (1) 28-29

Pelbois, F., 1955: Twelve cases of failure of the treatment of syphilis with penicillin aluminum monostearate alone. Maroc Medical 34(360): 563-564

Simon E., 1975: Vegetation dynamics in some metalliferous areas in the neighborhood of eupen and aachen in relation with edaphic factors. Bulletin de la Societe Royale de Botanique de Belgique 108(2): 273-286

Badolle, A., 1950: Vertebral epiphysitis treated by radiotherapy. Journal de Radiologie, D'electrologie & Archives D'electricite Medicale 31(9-10): 586-587