+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Different counteracting host immune responses to clade and Egyptian H5N1 highly pathogenic avian influenza viruses in naïve and vaccinated chickens

, : Different counteracting host immune responses to clade and Egyptian H5N1 highly pathogenic avian influenza viruses in naïve and vaccinated chickens. Veterinary Microbiology 183: 103-109

In Egypt, two distinct lineages of H5N1 highly pathogenic avian influenza (HPAI) viruses, "classic" and "variant" strains, have evolved. The underlying host immune responses counteracting these viruses in chickens remain not well understood. In the present study, the cytokine responses to a classic strain (C121) and those to a variant strain (V1063) were compared in naïve and vaccinated chickens. In naïve chickens, the C121 replicated more efficiently than the V1063. Both the C121 and the V1063 increased interferon (IFN)-γ and interleukin (IL)-10 gene expression at 48 h post inoculation (hpi) in the lung and spleen but the levels of these cytokines were lower in chickens infected with the C121 than those infected with the V1063. In contrast, in chickens vaccinated with inactivated C121-based vaccine, the C121 replicated less than the V1063. Both challenge with the C121 and that with the V1063 did not increase IFN-γ gene expression at 48 hpi; rather, the C121 increased IL-4 gene expression in the lung accompanied with lower viral titer and higher HI titers. These results suggested that the pathogenicity of HPAI viruses correlated with IFN-γ-producing helper and/or cytotoxic T cell responses in naïve chickens, whereas vaccine efficacy to HPAI viruses correlated with IL-4 producing helper T cell responses in the lung in vaccinated chickens. It implies that IL-4 in the lung, in addition to the traditional serum HI titers, could be used to screen novel vaccine strategies, such as strains, adjuvant, prime/boost protocols, against HPAI in chickens.


PMID: 26790942

DOI: 10.1016/j.vetmic.2015.12.005

Other references

Christ, J.F.; Jarosch, E., 1969: Quantitative investigations on the effect of pentamethylenetetrazol on the supraoptic neurones in rabbit. Zeitschrift für Zellforschung und Mikroskopische Anatomie 93(3): 404-413

Frenn, M., 2012: Normal weight is associated with significant reductions in cardiovascular risk for adults, even in those who were overweight or obese as children. Evidence-Based Nursing 15(3): 69-70

Mcgovern, J.N.; Evert, J.N.; Chidester, G.H., 1941: Absorption of chemicals from neutral sodium sulphite liquor by sweetgum chips. Studies of some of the conditions affecting the absorption of Na2SO3-Na2CO3 liquors by sweet-gum (Liquidambar styraciflua) chips, in reference to their subsequent partial pulping for a semichemical pulp product, reveal that the time and temp. of i...

Panov, I.M., 2003: Soil cultivation equipment, status and development. The trend towards using chisel ploughs and deep-loosening soil-slitters (instead of implements which invert the soil) is discussed, in the context of soil conservation and energy saving. The advantages of rotary (power take-off driven) ploughs and...

Lacapère, J.J.; Garin, J.; Trinnaman, B.; Green, N.M., 1993: Identification of amino acid residues photolabeled with 8-azidoadenosine 5'-diphosphate in the catalytic site of sarcoplasmic reticulum Ca-ATPase. The photoreactive ADP analogue 8-N3-ADP binds in the dark to the catalytic site of the sarcoplasmic reticulum Ca-ATPase. An apparent Kd value of 30 mM has been deduced from competition with ADP in the presence of EGTA. Photoirradiation of Ca-ATPas...

Goindi, S.; Kumar, G.; Kaur, A., 2015: Novel flexible vesicles based topical formulation of levocetirizine: in vivo evaluation using oxazolone-induced atopic dermatitis in murine model. Levocetirizine, an active enantiomer of cetirizine is third generation antihistaminic agent used for treating various allergies like atopic dermatitis, chronic idiopathic urticaria and allergic rhinitis. Development of novel topical formulation of...

Weiner, M.H.; Coats-Stephen, M., 1979: Immunodiagnosis of systemic candidiasis: mannan antigenemia detected by radioimmunoassay in experimental and human infections. A radioimmunoassay (RIA) that detects candida mannan was developed so that immunodiagnosis of systemic candidiasis could be improved. The RIA was evaluated in an animal model of disseminated disease and in a panel of patient sera. Mannan antigenem...

Janssen, T.; Karssen, G.; Topalović, O.; Coyne, D.; Bert, W., 2017: Integrative taxonomy of root-knot nematodes reveals multiple independent origins of mitotic parthenogenesis. During sampling of several Coffea arabica plantations in Tanzania severe root galling, caused by a root-knot nematode was observed. From pure cultures, morphology and morphometrics of juveniles and females matched perfectly with Meloidogyne africa...

Verma M., 1979: Mymar schwanni new record from india hymenoptera chalcidoidea mymaridae. Journal of the Bombay Natural History Society 76(3): 535-536

Guernsey, F.W., 1946: The utilization of Western Hemlock sawmill waste in British Columbia. Studies made at the Forest Products Laboratory, Vancouver, show that during the manufacture of Fir and Hemlock logs into lumber in sawmills of the coast region of British Columbia the following 'waste' is produced for every 1, 000 bd. ft...