geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Direct Protocol for Ambient Mass Spectrometry Imaging on Agar Culture


, : Direct Protocol for Ambient Mass Spectrometry Imaging on Agar Culture. Analytical Chemistry 87(13): 6925-6930

Herein we describe a new protocol that allows direct mass spectrometry imaging (IMS) of agar cultures. A simple sample dehydration leads to a thin solid agar, which enables the direct use of spray-based ambient mass spectrometry techniques. To demonstrate its applicability, metal scavengers siderophores were imaged directly from agar culture of S. wadayamensis, and well resolved and intense images were obtained using both desorption electrospray ionization (DESI) and easy ambient sonic-spray ionization (EASI) with well-defined selective spatial distributions for the free and the metal-bound molecules, providing clues for their roles in cellular metabolism.

US$19.90

PMID: 26067682

DOI: 10.1021/acs.analchem.5b01538


Other references

Schildhauer, T.A.; Robie, B.; Muhr, G.; Köller, M., 2006: Bacterial adherence to tantalum versus commonly used orthopedic metallic implant materials. Evaluation of bacterial adhesion to pure tantalum and tantalum-coated stainless steel versus commercially pure titanium, titanium alloy (Ti-6Al-4V), and grit-blasted and polished stainless steel. Experimental in vitro cell culture study using Stap...

Ware, J.E., 2007: Improvements in short-form measures of health status: introduction to a series. Journal of Clinical Epidemiology 61(1): 1-5

Voĭtkevich, A.A.; Soboleva, E.L., 1971: Adenohypophyseal lobes following acute radiation injury. Izvestiia Akademii Nauk Sssr. Seriia Biologicheskaia 1: 56-59

Hari, P., 1909: Der respiratorische Gaswechsel der winterschlafenden Fledermaus. Archiv fuer die Gesamte Physiologie Bonn, 130 112-133

Vlasenko, S.Y.; Lebedin, Y.S., 1992: Development and use of an ELISA-based method of specific IgG determination for diagnosis of pulmonary aspergillosis. An ELISA-based assay to determine IgG antibodies to Aspergillus fumigatus antigens was developed. Sera from patients with aspergilloma and invasive aspergillosis and from healthy control patients were used to standardize the method. Filtrate of a...

Chalabala, M.; Rak, J.; Vitková, M.; Hederová, S., 1995: Drug forms for drug delivery into the gastrointestinal tract. Most drugs introduced into the GIT are absorbed into the stomach and/or the small intestine according to their chemical character. Some drugs (their number is on the increase) require targeted supply to a certain site in the GIT if their biologica...

Y.C.iHong; Gao XiWu, 2005: Partial purification of Cytochrome P450 from Helicoverpa armigera (Hubner). The cytochrome P450 (Cyt-P450) proteins from the fat body and midgut of the cotton bollworm, Helicoverpa armigera, were respectively partially purified by a set of purification procedures including differential centrifugation, solubilization of CH...

de Boer, G.; Bressmann, T., 2015: Application of Linear Discriminant Analysis to the Long-term Averaged Spectra of Simulated Disorders of Oral-Nasal Balance. Acoustic studies of oral-nasal balance disorders to date have focused on hypernasality. However, in patients with cleft palate, nasal obstruction may also be present, so that hypernasality and hyponasality co-occur. In this study, normal speakers...

Bussian, R.W.; Ruglass, J.A., 1994: Method for determining lactate dehydrogenase isoenzyme LD1 by stabilizing LD1 and inactivating isoenzymes LD2-LD5. Official Gazette of the United States Patent & Trademark Office Patents 1169(2): 1105, Dec 13

Xia, J-Jing.; Li, M-Sen.; Zheng, L.; Shi, Y-Zhen., 2017: Nondiagnostic cytological results on ultrasound-guided fine needle aspiration: Does the thyroid nodule depth matter?. To evaluate whether thyroid nodule depth and other ultrasound features can predict nondiagnostic cytological results on ultrasound-guided fine needle aspiration. This retrospective study included 412 thyroid nodules that underwent ultrasound-guide...