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Dissection of quantitative traits by bulk segregant mapping in a protoploid yeast species

, : Dissection of quantitative traits by bulk segregant mapping in a protoploid yeast species. Fems Yeast Research 16(5): -

Since more than a decade ago, Saccharomyces cerevisiae has been used as a model to dissect complex traits, revealing the genetic basis of a large number of traits in fine detail. However, to have a more global view of the genetic architecture of traits across species, the examination of the molecular basis of phenotypes within non-conventional species would undoubtedly be valuable. In this respect, the Saccharomycotina yeasts represent ideal and potential non-model organisms. Here we sought to assess the feasibility of genetic mapping by bulk segregant analysis in the protoploid Lachancea kluyveri (formerly S. kluyveri) yeast species, a distantly related species to S. cerevisiae For this purpose, we designed a fluorescent mating-type marker, compatible with any mating-competent strains representative of this species, to rapidly create a large population of haploid segregants (>10(5) cells). Quantitative trait loci can be mapped by selecting and sequencing an enriched pool of progeny with extreme phenotypic values. As a test bed, we applied this strategy and mapped the causal loci underlying halotolerance phenotypes in L. kluyveri Overall, this study demonstrates that bulk segregant mapping is a powerful way for investigating the genetic basis of natural variations in non-model yeast organisms and more precisely in L. kluyveri.


PMID: 27371856

DOI: 10.1093/femsyr/fow056

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Willmann, R.; Fuhrer, C., 2002: Neuromuscular synaptogenesis: Clustering of acetylcholine receptors revisited. Clustering of neurotransmitter receptors in the postsynaptic membrane is critical for efficient synaptic transmission. During neuromuscular synaptogenesis, clustering of acetylcholine receptors (AChRs) is an early sign of postsynaptic differentiat...

Andersson, B.; Dahmén, J.; Frejd, T.; Leffler, H.; Magnusson, G.; Noori, G.; Edén, C.S., 1983: Identification of an active disaccharide unit of a glycoconjugate receptor for pneumococci attaching to human pharyngeal epithelial cells. Glycoconjugates containing the disaccharide unit GlcNAc beta 1 leads to 3Gal beta were suggested as receptors for pneumococci adhering to human pharyngeal epithelial cells. The receptor activity was detected both by inhibition of adhesion by an ex...

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Mcdonald, Ian, 1999: From pathogenesis to symptomatology. Thompson, A [Editor], McDonald, I [Editor] Key advances in the effective management of multiple sclerosis 1-4

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Sedletsky, I.D., 1942: New data on the crystalline structure of humic acid.. Evidence from X-ray diagrams and electronographic analysis indicates a crystalline structure for natural humic acid. Three Debye rings are shown, the second and third of which are very near to the two rings shown by artificial humic acid prepared...

Hallez, P., 1883: Sur la Spermatogenese et les phenomenes de la fecon dation chez PAscaris megalocephala. Bulletin Scientifique du Nord, VI 132-135

Valerio, V.; Polosa, P., 1950: Intravenous adrenalin in scaled doses and behavior of the blood sugar level. La Riforma Medica 64(12): 329-331

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