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Effect of optical clearing agents on optical coherence tomography images of cervical epithelium

, : Effect of optical clearing agents on optical coherence tomography images of cervical epithelium. Lasers in Medical Science 30(2): 517-525

Optical coherence tomography (OCT) can be used as an adjunct to colposcopy in order to detect precancerous and cancerous cervical lesions. Optical clearing agents (OCAs) temporarily reduce the optical scattering of biological tissues. The purpose of this study was to investigate their influence on OCT imaging. OCT images were taken from unsuspicious and suspicious areas of fresh conization specimens immediately after resection and 5, 10, and 20 min after application of dimethyl sulfoxide (DMSO) or polyethylene glycol (PEG). Corresponding histologies were obtained from all sites. The images taken 5, 10, and 20 min after application of OCA were compared to the initial images with respect to changes in brightness, contrast, and scanning depth using a standard nonparametric test of differences of proportions. Further, mean intensity backscattering curves were calculated from all OCT images in the histological groups CIN2, CIN3, inflammation, and normal epithelium. Mean difference profiles within each of these groups were determined, reflecting the mean differences between the condition before the application of OCA and the exposure times 5, 10, and 20 min, respectively. The null hypothesis was tested employing the Dicky-Fuller-test, Hotelings-test and run test. The visual analysis of 434 OCT images from 109 different sites of 24 conization specimens showed a statistically significant increase in brightness and contrast for normal and dysplastic epithelium after application of DMSO or PEG. Further, the analysis of mean intensity profiles suggests the existence of an increased backscattering intensity after application of DMSO or PEG. DMSO and PEG contribute substantially to optical clearing in cervical squamous epithelium and therefore influence OCT imaging in a positive way. With further refinement of the OCT technology, the observed changes may be beneficial in interpreting the tissue microstructure and identifying cervical intraepithelial neoplasia.


PMID: 25503301

DOI: 10.1007/s10103-014-1674-5

Other references

Carruth R.L.; Pool D.R., 1993: Hydrogeology of the Quitobaquito Spring and La Abra Plain area, Arizona and Sonora, Mexico. Eos, Transactions, American Geophysical Union 74(43, Suppl

Krakovskiĭ, M.E.; Ashirmetov, A.Kh., 1985: In vivo activity of drug-metabolizing enzymes in various pathological conditions. Activity of enzymes, metabolizing drugs, was studied in acute kidney insufficiency and acute purulent peritonitis of rabbits using antipyrine test. In these pathological states the antipyrine half-life was increased and clearance was decreased, th...

Chang, J.Y.; Han, H.J., 1998: Development and characterization of camptothecin-resistant human nasopharyngeal carcinoma cell line. Proceedings of the American Association for Cancer Research Annual Meeting 39: 422, March

M.Leese D.W., 1972: Initial experiments on growth of the american lobster in captivity. Fisheries Research Board of Canada Technical Report 320: 1-22

Santo, C.; Pista, A.; Bartolo, E.; Almeida, M.; Ayres, L.; Ferreira, P.; Rodrigo, G., 1992: Typing of human papillomavirus (HPV) in the male and female genitalia. Diagnosis and typing of HPV was started in November 1990 at the Virology department of the National Health Institute, Lisbon. Eighty one samples from 70 patients were studied, 57 from women observed at the cervico-vulvar out patient unit of the Gy...

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Lucas, W.J.; Nuccitelli, R., 1980: HCO 3 (-) and OH (-)transport across the plasmalemma ofChara : Spatial resolution obtained using extracellular vibrating probe. Internodal and whorl (branch) cells of the green alga,Chara corallina Klein ex Willd., em. R.D.W., were studied with the extracellular vibrating probe for measuring transmembrane ion currents, and with an extracellular pH microprobe for measuring...

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