+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Effects of disinfectants against norovirus virus-like particles predict norovirus inactivation

, : Effects of disinfectants against norovirus virus-like particles predict norovirus inactivation. Microbiology and Immunology 60(9): 609-616

Human noroviruses (NoVs) are a major cause of epidemic and sporadic acute gastroenteritis worldwide. Public and personal hygiene is one of the most important countermeasures for preventing spread of NoV infection. However, no a practicable cell culture system for NoV had been developed, initial tests of the virucidal effectiveness of anti-NoV disinfectants and sanitizers have been performed using surrogate viruses. In this study, NoV virus-like particles (VLPs) were used as a new surrogate for NoVs and a method for evaluating NoV inactivation using them developed. This method is based on morphological changes in VLPs after treatment with sodium hypochlorite. VLP specimens were found to become deformed and degraded in a concentration-dependent manner. Based on these results, the effects of sodium hypochlorite on VLPs were classified into four phases according to morphological changes and number of particles. Using the criteria thus established, the efficacy of ethanol, carbonates and alkali solutions against VLPs was evaluated. Deformation and aggregation of VLPs were observed after treatment with these disinfectants under specific conditions. To determine the degradation mechanism(s), VLPs were examined by SDS-PAGE and immunoblotting after treatment with sodium hypochlorite and ethanol. The band corresponding to the major capsid protein, VP1, was not detected after treatment with sodium hypochlorite at concentrations greater than 500 ppm, but remained after treatment with ethanol. These results suggest that VLPs have excellent potential as a surrogate marker for NoVs and can be used in initial virucidal effectiveness tests to determine the mechanism(s) of chemical agents on NoVs.


PMID: 27554301

DOI: 10.1111/1348-0421.12435

Other references

Gorman, M.; Kiang, Y.1; Chiang, Y.1; Palmer, R., 1982: Electrophoretic classification of the early maturity groups of named soybean cultivars Enzyme systems, Glycine max, Glycine soja. Soybean genetics newsletter United States Agricultural Research Service 9(9): 143-156

Bottorff, J.L.; Oliffe, J.L.; Sarbit, G.; Sharp, P.; Caperchione, C.M.; Currie, L.M.; Schmid, J.; Mackay, M.H.; Stolp, S., 2016: Evaluation of QuitNow Men: An Online, Men-Centered Smoking Cessation Intervention. Men continue to smoke cigarettes in greater numbers than women. There is growing evidence for the value of developing targeted, men-centered health promotion programs. However, few smoking cessation interventions have been designed for men. A gend...

Wenzel, S.L.; Bakhtiar, L.; Caskey, N.H.; Hardie, E.; Redford, C.; Sadler, N.; Gelberg, L., 1996: Dually diagnosed homeless veterans in residential treatment: service needs and service use. Journal of Nervous and Mental Disease 184(7): 441-444

Strada, R.; Candiollo, L., 1952: Considerations on a familial case of Paget's disease. Archivio Per le Scienze Mediche 94(3): 227-240

Yaghoobi, J.; Yates, J.L.; Williamson, V.M., 2005: Fine mapping of the nematode resistance gene Mi-3 in Solanum peruvianum and construction of a S. lycopersicum DNA contig spanning the locus. Currently, the only genetic resistance against root-knot nematodes in the cultivated tomato Solanum lycopersicum (Lycopersicon esculentum) is due to the gene Mi-1. Another resistance gene, Mi-3, identified in the related wild species Solanum peruv...

Dusci L.J.; Hackett L.P., 1976: Interference in dilantin assays. Clinical Chemistry 22(7): 1236

Brown, W.H.; Pearce, L., 1923: Studies Based On A Malignant Tumor Of The Rabbit : Iii. Intratesticular Transplantation And Clinical Course Of The Disease. The results obtained by intratesticular inoculation of a malignant tumor of the rabbit are presented in the form of a general summary covering the phenomena of growth and spontaneous regression of the tumor and the clinical evidence of disease in...

Vartanian, V.H.; Laude, D.A., 1995: High performance fourier transform ion cyclotron resonance mass spectrometry via a single trap electrode. An open-ended cylindrical cell with a single annular trap electrode located at the center of the excitation and detection region is demonstrated for Fourier transform ion cyclotron resonance mass spectrometry. A trapping well is created by applyin...

Brunetti, M.; Vecchi, G., 2003: Sul ritrovamento di Teretia elengatissima (Foresti, 1868) in terreni pliocenici dell'Emilia e della Toscana. The Authors would like make known the discovery in some localities of Emilia, and for the first time in two coeval localities of Tuscany too, of Teretia elegantissima (Foresti, 1868), an exclusive species of talian Pliocene, recorded only in the p...

Sherman, V.C., 1982: Taking over: notes to the new executive. Journal of Nursing Administration 12(5): 21-23