+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Efficient Production of γ-Polyglutamic Acid by Bacillus subtilis (natto) in Jar Fermenters

, : Efficient Production of γ-Polyglutamic Acid by Bacillus subtilis (natto) in Jar Fermenters. Bioscience, Biotechnology, and Biochemistry 61(10): 1684-1687

The large scale fermentation of γ-polyglutamic acid (γ-PGA) by Bacillus subtilis (natto) was done using a 30-liter jar fermenter. A stable cultivation without foaming could be done with addition of 3% NaCl to the medium. The γ-PGA productivity became higher with increasing speed of agitation and amounts of glutamic acid added to the broth. Finally, we were able to obtain about 35 mg/ml of γ-PGA under the optimum conditions. The glutamic acid added to the medium was efficiently converted into γ-PGA in the stationary phase. To discover the role of l-glutamic acid added to the medium for γ-PGA biosynthesis by Bacillus subtilis (natto), the radioactivity incorporated into γ-PGA from (14)C-l-glutamic acid was measured. As a result, radioactive γ-PGA was detected in the medium. Then, the glutamic acid in the medium was transported into the cells and actually polymerized as the glutamic acid unit of γ-PGA.


PMID: 27393164

DOI: 10.1271/bbb.61.1684

Other references

Fernández Girón, M.; Ferrer Marín-Blázquez, M.; Sánchez Suárez, J.L.; Minué Lorenzo, S.; López Morandeira, P.; Gijón Vidaurreta, P., 1991: Vaccine coverage against tetanus in the population over 30 years old cared for at a health center. To determine by questionnaire the incidence of effective vaccination against tetanus in the population aged over 30 years attending a health centre. As a secondary objective we wish to determine the patients who sought vaccination after immunoprop...

Brown, J.; Brickle, P.; Scott, B.E., 2013: Investigating the movements and behaviour of Patagonian toothfish Dissostichus eleginoides Smitt, 1898 around the Falkland Islands using satellite linked archival tags. Knowledge of the seasonal movements of Patagonian toothfish is an essential component for understanding their ecology and fisheries management. As only one demersal longline vessel participates in this fishery in Falkland's waters, over a vas...

Jacoby W.R.; Spittler E., 1983: The Earth in a wax model; experiments as model leading to the question; by what are continental plates animated?. Umschau in Wissenschaft und Technik 83(1): 26-30

Kalavati, C.Narasimhamurti, Cc, 1978: A new microsporidan, Octosporea porcellioi sp. n. from Porcellio laevus Latr (Oniscidae, Isooda, Crustaceae). Acta protozoologica7(3): 455-460

Luna, F.; Garcia-Moro, C.; Alonso, V., 2007: Life expectancy in a western and immigrant receiving population (El Ejido, SE Spain). This study deals with the mortality pattern of El Ejido population, an agricultural Spanish community with a sub-tropical climate, located on the western Mediterranean coast (Andalusia). Based on the incorporation of modern agrarian techniques, th...

Dehesh K.; Haeuser I.; Apel K.; Kloppstech K., 1983: The distribution of nadph proto chlorophyllide oxido reductase in relation to chlorophyll accumulation along the barley hordeum vulgare leaf gradient. The possible regulatory role of NADPH-protochlorophyllide oxidoreductase for chlorophyll accumulation was investigated in barley plants. Within the primary leaf of etiolated plants the different maturation stages of etioplasts are found in a linea...

McCowan, L.M.E.; Dekker, G.A.; Chan, E.; Stewart, A.; Chappell, L.C.; Hunter, M.; Moss-Morris, R.; North, R.A., 2009: Spontaneous preterm birth and small for gestational age infants in women who stop smoking early in pregnancy: prospective cohort study. To compare pregnancy outcomes between women who stopped smoking in early pregnancy and those who either did not smoke in pregnancy or continued to smoke. Prospective cohort study. Auckland, New Zealand and Adelaide, Australia. 2504 nulliparous wom...

Salomao, M.; Yu, W.M.; Brown, R.S.; Emond, J.C.; Lefkowitch, J.H., 2010: Steatohepatitic hepatocellular carcinoma (SH-HCC): a distinctive histological variant of HCC in hepatitis C virus-related cirrhosis with associated NAFLD/NASH. In explant livers with chronic hepatitis C (HCV-C) we have noted a distinctive histologic variant that we have termed steatohepatitic hepatocellular carcinoma (SH-HCC) with features resembling non-neoplastic steatohepatitis, including large drople...

Gutierrez O.; Arguello M.; Botero R.C.; Lastra G.; Ospina J.; Sierra F., 1989: Yellow phosphorus intoxication in rats effects of vitamin e. Gastroenterology 96(5 PART 2): A604

Biondo, E.; Jasper, A.; Biondo, E., 2003: Study of the conservation status of riparian forest of Harmonia Stream, Teutonia, Taquari valley, RS, and a possible proposal for rehabilitation with agroforestry systems. The study of the permanent preservation area of Harmonia Stream (Hydrological Basin Taquari-Antas), in Teutnia, located in the northeast region of Rio Grande do Sul, Brazil, was conducted in order to analyse the conservation and recomposition of t...