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Elevated H2 O2 levels in trinitrobenzene sulfate-induced colitis rats contributes to visceral hyperalgesia through interaction with the transient receptor potential ankyrin 1 cation channel

, : Elevated H2 O2 levels in trinitrobenzene sulfate-induced colitis rats contributes to visceral hyperalgesia through interaction with the transient receptor potential ankyrin 1 cation channel. Journal of Gastroenterology and Hepatology 31(6): 1147-1153

Inflammatory bowel disease is associated with chronic abdominal pain. Transient receptor potential ankyrin 1 (TRPA1) is a well-known pain sensor expressed in primary sensory neurons. Recent studies indicate that reactive oxygen species such as hydrogen peroxide (H2 O2 ) may activate TRPA1. Colonic inflammation was induced by intra-colonic administration of trinitrobenzene sulfate (TNBS) in adult male Sprague-Dawley rats. Visceromotor response (VMR) to colorectal distention (CRD) was recorded to evaluate the visceral hyperalgesia. Rats were sacrificed 1 day after treatment with saline or TNBS; colonic tissues from the inflamed region were removed and then processed to assess the H2 O2 content. H2 O2 scavenger N-acetyl-l-cysteine or a TRPA1 antagonist, HC-030031, was intravenously administrated to the TNBS-treated rats or saline-treated rats. In a parallel experiment, intra-colonic H2 O2 -induced visceral hyperalgesia in naïve rats and the effect of intravenous HC-030031 were measured based on the VMR to CRD. Trinitrobenzene sulfate treatment resulted in significant increase in VMR to CRD at day 1. The H2 O2 content in the inflamed region of the colon in TNBS-treated rats was significantly higher than that of saline-treated rats. N-acetyl-l-cysteine or HC-030031 significantly suppressed the enhanced VMR in TNBS-treated rats while saline-treated rats remained unaffected. Moreover, blockade of TRPA1 activation by HC-030031 significantly reversed the exogenous H2 O2 -induced visceral hyperalgesia. These results suggest that H2 O2 content of the colonic tissue is increased in the early stage of TNBS-induced colitis. The increased H2 O2 content may contribute to the visceral hyperalgesia by activating TRPA1.


PMID: 26574143

DOI: 10.1111/jgh.13226

Other references

Snodgrass, M.; Kalaida, N.; Winer, E.Samuel., 2009: Access is mainly a second-order process: SDT models whether phenomenally (first-order) conscious states are accessed by reflectively (second-order) conscious processes. Access can either be first-order or second-order. First order access concerns whether contents achieve representation in phenomenal consciousness at all; second-order access concerns whether phenomenally conscious contents are selected for metacog...

Binel, Gève.; Thibault, P., 2003: 3/6 Gastric tube placement. Soins. Pediatrie, Puericulture: 43-44

L.V.cchia, C.; Negri, E.; Pagano, R., 1990: Recent decline of smoking in younger Italian women. International Journal of Epidemiology 19(1): 221-221

Majde J.A.; Cohen E.P., 1972: The effect of lymphocytic choriomeningitis virus infection on the response of mouse spleen cells to plant mitogens. Abstracts of the Annual Meeting of the American Society for Microbiology 72: 195

Sankaranarayan, N.S., 1941: Flies and their Control in Cattle Farms and Dairies. A short account of fly control, mainly of Musca domestica and Stomoxys calcitrans. The breeding habits of these species are briefly dealt with. Methods for the prevention of breeding of the flies are described.

Larrambebere, W.; Costa, C., 1983: Genetic structure of the Brazil Hampshire swine herd. Pesquisa agropecuaria brasileira8(10): 1139-1146

Qiao, M.; Rushforth, D.; Wang, R.; Shaw, R.Anthony.; Tomanek, B.; Dunn, J.F.; Tuor, U.I., 2006: Blood-oxygen-level-dependent magnetic resonance signal and cerebral oxygenation responses to brain activation are enhanced by concurrent transient hypertension in rats. Neuronal activation results in increases in blood- oxygen- level- dependent ( BOLD) signal increases in magnetic resonance images, increases in cerebral blood flow ( CBF), and changes in tissue oxygenation. We hypothesized that transient hyperten...

Jalaluddin, M.; Gao, L.; Sethuraman, V.S., 2002: Modeling PK dose response in Phase I studies. Clinical Pharmacology & Therapeutics 71(2): P90, February

Common, T.G.; Wright, I.A.; Grant, S.A., 1998: The effect of grazing by cattle on animal performance and floristic composition in Nardus-dominated swards. Four groups of six spring-calving beef cows and their calves were allocated to two replicated grazing treatments on a Nardus stricta-dominated hill pasture in the UK during six consecutive summer grazing seasons. Treatments were applied by continu...

Grossheim, G.; Meister, W.; Tomsing, O.; Tonn, H., 2012: Closure of bronchial fistulas using amplatzer vascular plugs. Bronchial fistulas occurring either spontaneously in the course of malignant diseases of the chest or following pulmonary resections (lobectomy, pneumonectomy) are rare, but feared complications associated with high morbidity and mortality. In man...