geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Endothelium dependent hyperpolarization-type relaxation compensates for attenuated nitric oxide-mediated responses in subcutaneous arteries of diabetic patients


, : Endothelium dependent hyperpolarization-type relaxation compensates for attenuated nitric oxide-mediated responses in subcutaneous arteries of diabetic patients. Nitric Oxide 53: 35-44

Diabetes impairs endothelium-dependent relaxations. The present study evaluated the contribution of different endothelium-dependent relaxing mechanisms to the regulation of vascular tone in subcutaneous blood vessels of humans with Type 2 diabetes mellitus. Subcutaneous arteries were isolated from tissues of healthy controls and diabetics. Vascular function was determined using wire myography. Expressions of proteins were measured by Western blotting and immunostaining. Endothelium-dependent relaxations to acetylcholine were impaired in arteries from diabetics compared to controls (P = 0.009). Acetylcholine-induced nitric oxide (NO)-mediated relaxations [in the presence of an inhibitor of cyclooxygenases (COX; indomethacin) and small and intermediate conductance calcium-activated potassium channel blockers (UCL1684 and TRAM 34, respectively)] were attenuated in arteries from diabetics compared to controls (P < 0.001). However, endothelium-dependent hyperpolarization (EDH)-type relaxations [in the presence of indomethacin and the NO synthase blocker, l-NAME] were augmented in arteries from diabetics compared to controls (P = 0.003). Endothelium-independent relaxations to sodium nitroprusside (NO donor) and salbutamol (β-adrenoceptor agonist) were preserved, but those to prostacyclin were attenuated in diabetics compared to controls (P = 0.017). In arteries of diabetics, protein expressions of endothelial NO synthase, prostacyclin synthase and prostacyclin receptors were decreased, but those of COX-2 were increased. These findings suggest that in human diabetes, the impairment of endothelium-dependent relaxations is caused by a diminished NO bioavailability; however, EDH appears to compensate, at least in part, for this dysfunction.

US$19.90

PMID: 26768833

DOI: 10.1016/j.niox.2015.12.007


Other references

Porteres, R., 1965: Some singular new agrobotanic forms of African rice (O. glaberrima Steudel). The new taxa are: (1) ssp. vera proles mixta var. evoluta f. evoluta; (2) ssp. confusa proles confuso-mixta var. incompleta L incompleta; (3) ssp. vera proles senegambica var. rigida f. neo-luteobarbata; (4) ssp. confusa proles confuso-senegambica...

Tazhibaev S.S.; Mamyrbaev A.A.; Takhtaev F.Kh, 1986: Phospholipid composition of the lipids of subcellular structure membranes in the liver of rats given various diets and exposed to the action of phosphorus and fluorine compounds. A study was made of the phospholipid composition of the membranes of the rat liver nucleus, mitochondria and microsomes under the effect of different nutrition, hydrogen fluoride and phosphine. It was established that prolonged exposure to hydroge...

Flerov, B.C.; Popov, G.A., 1933: Methods for the investigation of the action of antiseptic preparations on wood-destroying fungi. All the current methods of testing timber preservatives are stated to be open to a number of objections. The writers therefore devised the following procedure based as far as possible on natural conditions. A layer of garden soil is placed in an E...

Panavas, T.; Pogany, J.; Nagy, P.D., 2002: Analysis of minimal promoter sequences for plus-strand synthesis by the Cucumber necrosis virus RNA-dependent RNA polymerase. Tombusviruses are small, plus-sense, single-stranded RNA viruses of plants. A partially purified RNA-dependent RNA polymerase (RdRp) preparation of cucumber necrosis virus (CNV), which is capable of de novo initiation of complementary RNA synthesi...

Ahmed, L.; Martin-Diana, A.B.len; Rico, D.; Barry-Ryan, C., 2012: Quality and Nutritional Status of Fresh-Cut Tomato as Affected by Spraying of Delactosed Whey Permeate Compared to Industrial Washing Treatment. The aim of this study was to examine the applicability of delactosed whey permeate (DWP) treatment on preserving the quality and antioxidant attributes of fresh-cut tomato. Tomatoes were treated with 3% DWP by dipping, spraying and a combination o...

WangLiXin; Kgope,B.; D'Odorico,P.; Macko,S.A., 2008: Carbon and nitrogen parasitism by a xylem-tapping mistletoe along the Kalahari Transect a stable isotope study. The present study explores the xylem-tapping parasitism by mistletoe (<i>Tapinanthus oleifolius</i>) on native tree species along the Kalahari Transect (KT) using the stable isotopes of carbon and nitrogen. Mistletoe-host pairs were co...

Rozhon, W.; Baubec, T.; Mayerhofer, J.; Mittelsten Scheid, O.; Jonak, C., 2008: Rapid quantification of global DNA methylation by isocratic cation exchange high-performance liquid chromatography. The DNA of many eukaryotes is methylated at specific cytosine residues in connection with gene regulation. Here we report a method for the quantification of global cytosine methylation based on enzymatic hydrolysis of DNA, dephosphorylation, and s...

Ehmcke, J.; Wistuba, J.; Clemen, Günter., 2004: Gender-dependent dimorphic teeth in four species of Mesoamerican plethodontid salamanders (Urodela, Amphibia). The tooth shapes of premaxillary and maxillary teeth of adults of four Mesoamerican salamander species (Urodela, Plethodontidae) were examined. Using scanning electron microscopy we determined whether the monocuspid teeth that are present only on...

Powell, R.L.; Westlake, W.J.; Longaker, E.D.; Greene, L.C., 1971: A clinical evaluation of a new concentrated antacid. II. Safety and efficacy in duodenal ulcer patients. Journal of Clinical Pharmacology and New Drugs 11(4): 296-300

Liu, Y.; Yang, P.; Hu, C.; Guo, H., 2008: Water quality modeling for load reduction under uncertainty: a Bayesian approach. A Bayesian approach was applied to river water quality modeling (WQM) for load and parameter estimation. A distributed-source model (DSM) was used as the basic model to support load reduction and effective water quality management in the Hun-Taizi...