geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Enhanced Enzymatic Preparation of Biodiesel Using Ricinoleic Acid as Acyl Donor: Optimization Using Response Surface Methodology


, : Enhanced Enzymatic Preparation of Biodiesel Using Ricinoleic Acid as Acyl Donor: Optimization Using Response Surface Methodology. Journal of Oleo Science 65(9): 785-795

Castor oil methyl ester is a kind of biodiesel from castor oil. However, in those previous methods for biodiesel preparation using castor oil as feedstock, glycerol was the main by-product, which had a strong blocking effect on the immobilized enzyme activity and affected the mass transfer of reaction system. For avoiding the negative effect of glycerol on the enzymatic esterification, biodiesel was prepared using ricinoleic acid (RA) as acyl donor. Enzyme screening was also studied, and the effects of reaction temperature, molar ratio of ricinoleic acid and methanol, enzyme load, and reaction time, on the preparation of castor methyl ester were also evaluated. Response surface methodology (RSM) was used to optimize the interaction effect of reaction variables (reaction temperature (30-70°C), enzyme load (2-7%; relative to the weight of total substrates), molar ratio of methanol to ricinoleic acid (2:1-10:1), and reaction time (0.5-2.5 h)) on the acid value (AV) and the degree of esterification (DE). Validation of the RSM model was verified by the good agreement between the experimental and the predicted values of AV and DE. The optimum preparation conditions were as follows: reaction temperature, 48.2°C; enzyme load, 5.8%; molar ratio of methanol to ricinoleic acid, 5.56:1; reaction time, 2.36 h. Under these conditions, the AV and DE of the esterification reaction are 10.36±1.05 mgKOH/g and 94.03±0.60%, respectively. The relationship between initial reaction rate and temperature was also established, and the activation energy (Ea) of the enzymatic esterification is 33.87 KJ/mol.

US$19.90

PMID: 27477073

DOI: 10.5650/jos.ess16052


Other references

U.Preventive Services Task Force, 1993: Screening for adolescent idiopathic scoliosis Review article. Jama (journal Of The American Medical Association). 269(20): 2667-2672

Sackler M.L., 1968: Morphological changes in mitochondria and associated organelles during development of the pre implantation mouse embryo abstract. Journal of Cell Biology 39(2 PT 2): 117A

Wagstaff, K.J., 1986: The editors interview Kenneth J. Wagstaff. New England Journal of Human Services 5(4): 6-11

Velho, E.L., 1958: Salmonella organisms in fish meal in Angola. The incidence of salmonella in batches of fish meal samples in Angola varies between 8% and 40%. Salmonella was isolated from 26 of 161 samples of faeces from fishery workers, from the intestine of 2 of 7 birds, and from 2 of 20 batches of house-f...

Suter, P.M., 1997: Therapeutic strategies leading to a better lung function in severe acute pulmonary failure. Intensivmedizin und Notfallmedizin 34(5): 462-465

Sopinska, A., 1987: Identification of white blood cells of the carp and the trout on the basis of cytochemical test. The aim of the work was the identification of phagocytic cells (granulocytes, monocytes and macrophages) and T and B lymphocytes in the carp (Cyprinus carpio) and in the trout (Salmo gairdneri), using cytochemical tests. The chosen tests enabled t...

Archer M.C.; Labuc G.E., 1983: The mode of action of n nitrosomethyl benzylamine an esophageal carcinogen in the rat. Magee, P N (Ed ) Banbury Report, Vol 12 Nitrosamines And Human Cancer; Meeting Xvi+599p Cold Spring Harbor Laboratory: Cold Spring Harbor, N Y , Usa Illus P87-102, 1982

Burns, J.J.; Cucinell, S.A.; Koster, R.; Conney, A.H., 1965: Application Of Drug Metabolism To Drug Toxicity Studies. Annals of the New York Academy of Sciences 123: 273-286

Hickey, A.; Holly, D.; McGee, H.; Conroy, R.; Shelley, E., 2012: Knowledge of stroke risk factors and warning signs in Ireland: development and application of the Stroke Awareness Questionnaire (SAQ). Inability to recognize stroke warning signs and delay in seeking medical attention for recognized symptoms contribute to treatment delay, thus limiting the potential for intervention and impacting negatively on potential stroke outcome. To examine...

Otterloo, R.H. van; Kuijpers, J.W.M., 1985: Contamination of the sediment in aquatic systems. The results of riverbed drillings in the northern delta basin of Holland are discussed in relation to the closure of the Haringvliet by dam in 1970. The levels of organic pollutants and heavy metals in the estuarine ecosystem give cause for concer...