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Expansion and function of MHC restricted killer T cells derived from umbilical cord blood


, : Expansion and function of MHC restricted killer T cells derived from umbilical cord blood. Zhongguo Shi Yan Xue Ye Xue Za Zhi 23(1): 195-201

This study was to expand the cytotoxic T lymphocytes (CTL) through inducing the differentiation of umbilical blood monomuclear cells (UBMNC) by using various combination of cytokines, and to investigate the functions of expanded CTL. The MNC were isolated by ficoll density gradient centrifugation. Then, the PHA-P, IFN-γ combined with IL-2, IL-15 and other cytokines were used for induction and expansion of the cord blood-derived CTL. The biological function of CTL was examined by phenotype analysis, cytotoxic tests and real-time fluorescence quantitative PCR. After expansion for 15 days, the cell number increased by 1522% ± 137%. The content of CD3(-)CD8(-) cells in uncultured cord blood MNC was 95%, and the CD3(+)CD8(+) CTL cells reached 82.77% in cultured cord blood MNC after expansion for 15 days. The expanded CTL cell showed the cytotoxic activity against K562 and HeLa cell line. The killing rate of MNC was 61.88 ± 1.08%. After expansion, the killing rate could reach to 90% with the average value of 90.33 ± 2.02%. The expanded CTL cells highly expressed some key cytokines, such as granzyme A, granzyme B, GM-CSF, granulysin, IFN-γ, TGF-β, TNF-α and perforin. Compared with the control group, the expression of IFN-γ and TGF-β significantly increased (P < 0.05), and the other factors dramatically increased (P < 0.01). The cord blood-derived CTL can be expanded by different combinations of cytokines. These protocols may provide alternative choices for CTL cell expansion in tumor adoptive immunotherapy.

US$29.90

PMID: 25687072


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Tseng, Y-Ming.; Jin, Y-Ru.; Chen, I-Jun.; Huang, F-Dee.; Wu, S-Hsien.; Ma, H.; Chen, S-Yi.; Tsai, L-Yu.; Tsai, S-Meng.; Lee, J-Hwa., 2007: Roles of the genetic variation of alcohol-metabolizing enzymes on biomarkers in trauma patients with excessive alcohol intake at emergency department. Background: Alcohol abuse has been implicated as an important factor for accidents. We evaluated the roles of different genetic combinations of the ADH2 and ALDH2 genotypes on biomarkers in trauma patients with excessive alcohol intake at our emer...

Icart Isern, M.T., 1990: The bibliographic review (I). Essential documentary sources for nursing. Revista de Enfermeria 13(148): 15-19

Kubo T.; Kihara M.; Misu Y., 1991: Ipsilateral but not contralateral blockade of excitatory amino acid receptors in the caudal ventrolateral medulla inhibits aortic baroreceptors reflex in rats. The caudal ventrolateral medulla (CVLM) contains vasodepressor neurons which, when activated, decrease vasomotor tone. To investigate whether excitatory amino acid receptors in the CVLM of the rat are involved in mediation of the aortic barorecept...

Kaushal, K., 2016: Calculating incidence and prognosis in a prospective study. Indian Journal of Critical Care Medicine 20(7): 434-435

Schneeberg, N.G.; Woolhandler, G.; Levine, R., 1947: The clinical significance of hyperostosis frontalis interna. Journal of Clinical Endocrinology and Metabolism 7(9): 624-635

Svab, V., 1955: Conference on early diagnosis of tumors of the kidneys and of the urinary tract. Ceskoslovenska Rentgenologie 9(4): 141-143

Garcia, J.F.; Villuendas, R.; Sanchez Beato, M.; Sanchez, L.; Piris, M.A., 2001: Nucleolar p14ARF overexpression in Sternberg-Reed cells in Hodgkins lymphoma. Laboratory Investigation 81(1): 163A, January

Anonymous, 2001: The human genome map: reality and fantasy. Revista de Derecho Y Genoma Humano 12): 15-18

Moussa Mounir T., 1969: Green River Formation (Eocene) in the Soldier Summit area, Utah. Stratigraphic units, several new names, delta facies, upper boundary, correlations, summarized stratigraphic chart for Uinta basin.

Lopes, M.C.; Laborda, R., 1994: Palliative nursing is looking for a new vision of patient care. Enfoque 21(3-4): 28-29