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Facilitated Diagnosis of Pneumothoraces in Newborn Mice Using X-ray Dark-Field Radiography

, : Facilitated Diagnosis of Pneumothoraces in Newborn Mice Using X-ray Dark-Field Radiography. Investigative Radiology 51(10): 597-601

The aim of this study was to evaluate the diagnostic value of x-ray dark-field imaging in projection radiography-based depiction of pneumothoraces in the neonatal murine lung, a potentially life-threatening medical condition that requires a timely and correct diagnosis. By the use of a unique preclinical model, 7-day-old C57Bl/6N mice received mechanical ventilation for 2 or 8 hours with oxygen-rich gas (FIO2 = 0.4; n = 24). Unventilated mice either spontaneously breathed oxygen-rich gas (FIO2 = 0.4) for 2 or 8 hours or room air (n = 22). At the end of the experiment, lungs were inflated with a standardized volume of air after a lethal dose of pentobarbital was administered to the pups. All lungs were imaged with a prototype grating-based small-animal scanner to acquire x-ray transmission and dark-field radiographs. Image contrast between the air-filled pleural space and lung tissue was quantified for both transmission and dark-field radiograms. After the independent expert's assessment, 2 blinded readers evaluated all dark-field and transmission images for the presence or absence of pneumothoraces. Contrast ratios, diagnostic accuracy, as well as reader's confidence and interreader agreement were recorded for both imaging modalities. Evaluation of both x-ray transmission and dark-field radiographs by independent experts revealed the development of a total of 10 pneumothoraces in 8 mice. Here, the contrast ratio between the air-filled pleural space of the pneumothoraces and the lung tissue was significantly higher in the dark field (8.4 ± 3.5) when compared with the transmission images (5.1 ± 2.8; P < 0.05). Accordingly, the readers' diagnostic confidence for the diagnosis of pneumothoraces was significantly higher for dark-field compared with transmission images (P = 0.001). Interreader agreement improved from moderate for the analysis of transmission images alone (κ = 0.41) to very good when analyzing dark-field images alone (κ = 0.90) or in combination with transmission images (κ = 0.88). Diagnostic accuracy significantly improved for the analysis of dark-field images alone (P = 0.04) or in combination with transmission images (P = 0.02), compared with the analysis of transmission radiographs only. The significant improvement in contrast ratios between lung parenchyma and free air in the dark-field images allows the facilitated detection of pneumothoraces in the newborn mouse. These preclinical experiments indicate the potential of the technique for future clinical applications.


PMID: 27603110

DOI: 10.1097/RLI.0000000000000285

Other references

Panayi, C.; Estey, R.H., 1971: Control of the root-knot nematode Meloidogyne hapla with the insecti-cide-nematicide Lannate. Lannate (methyl(methylcarbamoyl)thioacetimidate 90% w.p.) at 16, 32, 48 or 64 p.p.m., applied as a soil drench at transplanting, gave satisfactory control of root-knot of tomatoes grown in soil infested with M. hapla. Good root-knot control with n...

Cusack F.; Lappin Scott H.M.; Costerton J.W., 1989: Three dimensional modelling for oilfield microbiology a novel approach. Abstracts of the Annual Meeting of the American Society for Microbiology 89: 357

Lauder, G.V.; Nauen, J.C.; Drucker, E.G., 2002: Experimental Hydrodynamics and Evolution: Function of Median Fins in Ray-finned Fishes. The median fins of fishes consist of the dorsal, anal, and caudal fins and have long been thought to play an important role in generating locomotor force during both steady swimming and maneuvering. But the orientations and magnitudes of these for...

Brunner, D.B.; Desponds, G.; Biollaz, J.; Keller, I.; Ferber, F.; Gavras, H.; Brunner, H.R.; Schelling, J.L.; Brunner, D.B.; Desponds, G.; Biollaz, J.; Keller, I.; Ferber, F.; Gavras, H.; Brunner, H.R.; Schelling, J.L., 2004: Effect of a new angiotensin converting enzyme inhibitor MK 421 and its lysine analogue on the components of the renin system in healthy subjects. 1981. British Journal of Clinical Pharmacology 58(7): S778-84; Discussion S785-7

Rushbrook, J.I.; Stracher, A., 1979: Comparison of adult, embryonic, and dystrophic myosin heavy chains from chicken muscle by sodium dodecyl sulfate/polyacrylamide gel electrophoresis and peptide mapping. Chicken myosin heavy chains from adult fast white muscle fibers (both normal and dystrophic), adult slow red fibers, and embryonic presumptive fast white fibers were compared by sodium dodecyl sulfate/polyacrylamide gel electrophoresis and by pept...

Godfrey, M., 2004: Using PIDs in confined spaces. Occupational Health & Safety 73(2): 40-44

Broketa, M.M.; Jeren, T.; Mlinarić-Galinović, G., 2000: Herpes simplex viruses: biological characteristics, immunopathogenesis, diagnosis and treatment. Herpes simplex virus (HSV) is the most common cause of sporadic encephalitis in developed countries. Herpes simplex virus type 1 (HSV-1) causes oropharyngeal infections, keratoconjunctivitis and infections of the central nervous system, while herp...

Smith, T.B.; Baker, M.A.; Connaughton, H.S.; Habenicht, U.; Aitken, R.J., 2015: Functional deletion of Txndc2 and Txndc3 increases the susceptibility of spermatozoa to age-related oxidative stress. Oxidative stress in the male germ line is known to be a key factor in both the etiology of male infertility and the high levels of DNA damage encountered in human spermatozoa. Because the latter has been associated with a variety of adverse clinic...

Annim, S.Kobina.; Awusabo-Asare, K.; Amo-Adjei, J., 2016: Household Nucleation, Dependency And Child Health Outcomes In Ghana. This study uses three key anthropometric measures of nutritional status among children (stunting, wasting and underweight) to explore the dual effects of household composition and dependency on nutritional outcomes of under-five children in Ghana....

Kortsch, W.E., 1963: Council Adopts Labeling Requirement And A Change In A.D.A. Specification No.17. Council On Dental Research. Journal of the American Dental Association 67: 756-757