geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Functional characterisation of Schistosoma japonicum acetylcholinesterase


, : Functional characterisation of Schistosoma japonicum acetylcholinesterase. Parasites & Vectors 9(1): 328-328

Acetylcholinesterase (AChE) is an important metabolic enzyme of schistosomes present in the musculature and on the surface of the blood stage where it has been implicated in the modulation of glucose scavenging from mammalian host blood. As both a target for the antischistosomal drug metrifonate and as a potential vaccine candidate, AChE has been characterised in the schistosome species Schistosoma mansoni, S. haematobium and S. bovis, but not in S. japonicum. Recently, using a schistosome protein microarray, a predicted S. japonicum acetylcholinesterase precursor was significantly targeted by protective IgG1 immune responses in S. haematobium-exposed individuals that had acquired drug-induced resistance to schistosomiasis after praziquantel treatment. We report the full-length cDNA sequence and describe phylogenetic and molecular structural analysis to facilitate understanding of the biological function of AChE (SjAChE) in S. japonicum. The protein has high sequence identity (88 %) with the AChEs in S. mansoni, S. haematobium and S. bovis and has 25 % sequence similarity with human AChE, suggestive of a highly specialised role for the enzyme in both parasite and host. We immunolocalized SjAChE and demonstrated its presence on the surface of adult worms and schistosomula, as well as its lower expression in parenchymal regions. The relatively abundance of AChE activity (90 %) present on the surface of adult S. japonicum when compared with that reported in other schistosomes suggests SjAChE may be a more effective drug or immunological target against this species. We also demonstrate that the classical inhibitor of AChE, BW285c51, inhibited AChE activity in tegumental extracts of paired worms, single males and single females by 59, 22 and 50 %, respectively, after 24 h incubation with 200 μM BW284c51. These results build on previous studies in other schistosome species indicating major differences in the enzyme between parasite and mammalian host, and provide further support for the design of an anti-schistosome intervention targeting AChE.

US$19.90

PMID: 27283196

DOI: 10.1186/s13071-016-1615-1


Other references

Mikhailova, O.G.; Ivanov, Y.V., 1989: Experimental studies of determination of the pesticide Sumilex atherogenic danger. Biochemical and cytological investigations using rabbits confirmed the potential danger of Sumilex [procymidone] related to the development of atherosclerosis.

Kumar, D.S.; Jacob, K.S., 1994: Managing a violent patient. National Medical Journal of India 7(3): 140-142

Thompson, C.M.; Sonawane, B.; Grafström, R.C., 2009: The ontogeny, distribution, and regulation of alcohol dehydrogenase 3: implications for pulmonary physiology. Class III alcohol dehydrogenase (ADH3), also termed formaldehyde dehydrogenase or S-nitrosoglutathione reductase, plays a critical role in the enzymatic oxidation of formaldehyde and reduction of nitrosothiols that regulate bronchial tone. Conside...

Mowatt, M.R.; Dunnick, W.A., 1986: DNA sequence of the murine gamma 1 switch segment reveals novel structural elements. Immunoglobulin heavy chain switch regions are segments of DNA considered to be important in mediating class switching in B lymphocytes. Whereas these semgents vary in length among the different murine isotypes, their structural organization scheme...

Belak, Z.R.; Ovsenek, N., 2007: Assembly of the Yin Yang 1 transcription factor into messenger ribonucleoprotein particles requires direct RNA binding activity. The early stages of vertebrate development depend heavily on control of maternally transcribed mRNAs that are stored for long periods in complexes termed messenger ribonucleoprotein particles (mRNPs) and utilized selectively following maturation a...

Iida, Y., 1985: Splice-site signals of mRNA precursors as revealed by computer search. Site-specific mutagenesis and thalassemia. Many eukaryotic genes are interrupted by introns, which are removed from mRNA precursors by the RNA splicing mechanism. Although nucleotide sequences around splice sites have considerable homology among various genes, exact splice-site signals are...

Cheung, W.W.; Pitcher, T.J., 2005: Designing fisheries management policies that conserve marine species diversity in the northern South China Sea. This study suggests that, under data-limited conditions, dynamic ecosystem models can be used to explore fishery management policies that aim to conserve biodiversity. The northern South China Sea is a highly diverse ecosystem where rapid expansio...

Balta, S.; Demırkol, S.; Celik, T., 2014: Coenzyme Q10 supplementation may improve diastolic heart functions especially coronary artery disease patients. Hemodialysis International. International Symposium on Home Hemodialysis 17(3): 467-468

Podbregar, M.; Mozina, H., 2007: Skeletal muscle oxygen saturation does not estimate mixed venous oxygen saturation in patients with severe left heart failure and additional severe sepsis or septic shock. Low cardiac output states such as left heart failure are characterized by preserved oxygen extraction ratio, which is in contrast to severe sepsis. Near infrared spectroscopy (NIRS) allows noninvasive estimation of skeletal muscle tissue oxygenati...

Alexander, R.; Clements, J.A.; Guest, R.; Hailey, P.; Leiper, K.; Moffat, A.C.; Pugh, K., 1999: The new technologies forum. Journal of Pharmacy & Pharmacology 51(SUPPL ): 43, Sept