+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Gene Expression and Antiviral Activity of Interleukin-35 in Response to Influenza A Virus Infection

, : Gene Expression and Antiviral Activity of Interleukin-35 in Response to Influenza A Virus Infection. Journal of Biological Chemistry 291(32): 16863-16876

Interleukin-35 (IL-35) is a newly described member of the IL-12 family. It has been reported to inhibit inflammation and autoimmune inflammatory disease and can increase apoptotic sensitivity. Little is known about the role of IL-35 during viral infection. Herein, high levels of IL-35 were found in peripheral blood mononuclear cells and throat swabs from patients with seasonal influenza A virus (IAV) relative to healthy individuals. IAV infection of human lung epithelial and primary cells increased levels of IL-35 mRNA and protein. Further studies demonstrated that IAV-induced IL-35 transcription is regulated by NF-κB. IL-35 expression was significantly suppressed by selective inhibitors of cyclooxygenase-2 (COX-2) and inducible nitric-oxide synthase, indicating their involvement in IL-35 expression. Interestingly, IL-35 production may have suppressed IAV RNA replication and viral protein synthesis via induction of type I and III interferons (IFN), leading to activation of downstream IFN effectors, including double-stranded RNA-dependent protein kinase, 2',5'-oligoadenylate synthetase, and myxovirus resistance protein. IL-35 exhibited extensive antiviral activity against the hepatitis B virus, enterovirus 71, and vesicular stomatitis virus. Our results demonstrate that IL-35 is a novel IAV-inducible cytokine, and its production elicits antiviral activity.


PMID: 27307042

DOI: 10.1074/jbc.M115.693101

Other references

Cherniavskaia, R.M., 1959: The phage titre increase test as a method for the diagnosis of dysentery. Zhur Mikrobiol Epidemiol I Immunobiol [Transl] 30(9): 152

Koppenol, Dël.C.; Vermolen, F.J.; Niessen, F.B.; van Zuijlen, P.P.M.; Vuik, K., 2016: A biomechanical mathematical model for the collagen bundle distribution-dependent contraction and subsequent retraction of healing dermal wounds. NlmCategory="UNASSIGNED">A continuum hypothesis-based, biomechanical model is presented for the simulation of the collagen bundle distribution-dependent contraction and subsequent retraction of healing dermal wounds that cover a large...

Callahan, PS., 1990: Nonlinear infrared coherent radiation as an energy coupling mechanism in living systems. Unknown

Peleg, L.; Slor, H.; Cordova, T.; Ayalon, D.; Legum, C.; Rudick, A., 1986: Improved reliability of a serum alpha-fetoprotein enzyme immunoassay compared with a radioimmunoassay. During the 6-month period between September 1982 and February 1983, serum samples were obtained from 850 pregnant women attending the municipal family clinics in Tel Aviv. All serum samples were tested by alpha-fetoprotein (AFP) enzyme immunoassay...

Nagami, H.; Asanuma, S.; Yajima, N.; Usuda, M.; Hirosawa, M.; Shimizu, S., 2004: Dymron herbicide residues in paddy soil and channel water. Dymron [daimuron] residues were surveyed in three organic rice fields (A, B and C) in Nagano, Japan, and the persistence of these residues was followed up. The channel water was monitored for dymron contamination and the potency of soil contaminat...

Grigg, A.; Seymour, J.F.; Roberts, A.; Szer, J., 1999: 'Mini-allografts' for haematological malignancies: An alternative to conventional myeloablative marrow transplantation. Background: Considerable morbidity and mortality are consequences of the myeloablative chemoradiotherapy utilised in conventional allogeneic marrow transplantation. This has generally restricted such potentially curative treatment to patients <...

Lin, H.J.; Rutherford, T.R.; Anagnou, N.P.; Nienhuis, A.W., 1987: Tissue and developmental specificity of globin gene promoters: gene expression in human and mouse erythroleukemia and nonerythroid cells. Progress in Clinical and Biological Research 251: 175-187

Schifftner, C.; Schulteis, G.; Wallace, M.S., 2017: Effect of Intravenous Alfentanil on Nonpainful Thermally Induced Hyperalgesia in Healthy Volunteers. Experimental interventions that activate specific components of clinical pain are necessary for characterization of underlying mechanisms and pharmacology. Cutaneous hyperalgesia has been described that uses nonpainful heat to induce secondary hyp...

Marisavljevic, D., 2012: Clonogenic assays are of limited value in discriminating patients with myelodysplastic syndrome and patients with megaloblastic anemia. Acta Haematologica 127(2): 118-118

Gibson, R., 1988: Change from sheep to cattle promotes conifer spread. Review Tussock Grassland and Mountain Lands Institute: (45) 26-28