geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Glomerular cytokine expression in murine lupus nephritis


, : Glomerular cytokine expression in murine lupus nephritis. Clinical and Experimental Nephrology 20(1): 23-29

Aberrant expression of T helper cell (Th) cytokines is believed to play a central role in the pathogenesis of systemic lupus erythematosus (SLE). While the glomerulus is one of the major targets of lupus inflammation, little is known about the cytokine expression in glomeruli. The current study aimed to explore the profiles of Th cytokine gene expressions in isolated glomeruli of lupus-prone mice. Glomeruli were purified from lupus-prone MRL/lpr mice using the magnetic microbead method. Expressions of cytokine genes representing the Th subset and FoxP3 were examined using real-time polymerase chain reaction. Serum levels of these cytokines were also measured by enzyme-linked immunosorbent assay. MRL/n mice were used as controls. Histologic glomerular damages were scored semiquantitatively. To examine the role of TNF-α in glomerular damage, we administered etanercept, a TNF-α antagonist, into the subjects. Glomerular gene expressions of TNF-α in lpr mice increased with week postpartum and reached statistically significant levels at 16 weeks compared with those of the glomeruli from control mice. Expressions of IFN-γ, IL-4 and FoxP3 also increased, but the difference was not significant. There was a significant increase in serum levels of TNF-α, IFN-γ, and IL-17 and decrease in those of IL-4. Among the genes examined, TNF-α significantly correlated with glomerular damage score. Administration of etanercept did not affect glomerular cytokine expressions or proteinuria and failed to ameliorate histologic glomerular damages. Our data suggest that Th1 cytokines, especially TNF-α, are dominantly expressed in the glomeruli of lupus-prone mice, but its pathophysiological role remains unclear.

US$19.90

PMID: 25985839

DOI: 10.1007/s10157-015-1123-1


Other references

Ju, X.; Cao, X.; Sun, Y.; Wang, Z.; Cao, C.; Liu, J.; Jiang, J., 2013: Purification and characterization of a fibrinolytic enzyme from Streptomyces sp. XZNUM 00004. A fibrinolytic enzyme (SFE1) from Streptomyces sp. XZNUM 00004 was purified to electrophoretic homogeneity with the methods including ammonium sulfate precipitation, polyacrylamide gel, DEAE-Sepharose Fast Flow anion exchange and gel-filtration ch...

Dynes, R.A.; Moss, R.A.; Bray, A.R.; McAnulty, R.W., 2006: Effect of weaning age on growth rates of lambs infected by gastrointestinal parasites. Gastrointestinal parasitism is one of the most important challenges facing low chemical or organic livestock production systems but also conventional farmers as resistance to anthelmintics becomes more widespread. Young twin-born and reared lambs...

Boswell, S.B.; Burns, R.M., 1973: Determination of fruit bearing zones in California citrus trees. The results of studies with orange, grapefruit and lemon trees at 14 localities are tabulated. In almost every plot most fruit was produced on the southern half of the tree, except in Navel and Valencia groves at Woodlake where most fruit was prod...

Jin, S.; English, R.S.; Shively, J.M., 1994: Transformation of Thiobacillus intermedius by electroporation. Abstracts of the General Meeting of the American Society for Microbiology 94(0): 223

Song, J.F.; Rudder, F.F.; Vorburger, T.V.; Smith, J.H., 1995: Microform Calibration Uncertainties of Rockwell Diamond Indenters. National and international comparisons in Rockwell hardness tests show significant differences. Uncertainties in the geometry of the Rockwell diamond indenters are largely responsible for these differences. By using a stylus instrument, with a ser...

Bella, H.; Gad, H.Abu., 2007: Twelve years experience with an innovative journal of family and community medicine: a self-assessment approach. Journal of Family & Community Medicine 14(1): 1-2

Bailey J.C., 1984: Geochemistry and origin of hornblende-bearing xenoliths in the I-type Petford Granite, North-east Queensland. Australian Journal of Earth Sciences: , Pages 7-23. 1984.

Marszałek, A.; Bartkowiak, Gżyna.; Dąbrowska, A.; Krzemińska, S.; Łężak, K.; Makowski, K.; Bugajska, J., 2017: Mine rescuers' heat load during the expenditure of physical effort in a hot environment, using ventilated underwear and selected breathing apparatus. Mine rescuers' heat load under the same physical effort load (25% of the maximal oxygen uptake), using three types of breathing apparatus, in newly developed heat-removing underwear and outerwear was assessed for typical work conditions of mi...

Price A.B.; Williams G.; Thompson H.; Unwin B., 1985: Pathologists problems in the recognition of early gastric cancer and gastric dysplasia. Gut 26(10): A1151

Staňová, A.Vojs.; Hrenáková, M.; Marák, J., 2015: Long-term analyses in automated electrophoretic analyzer in hydrodynamically closed separation system. Some potential problems that can occur during the analyses of complex samples by on-line combination of capillary isotachophoresis-capillary zone electrophoresis (cITP-CZE) in automated electrophoretic analyzer with the column-coupling configurati...