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Heterodimeric BMP-2/7 for nucleus pulposus regeneration-In vitro and ex vivo studies

, : Heterodimeric BMP-2/7 for nucleus pulposus regeneration-In vitro and ex vivo studies. Journal of Orthopaedic Research

NlmCategory="UNASSIGNED">Intervertebral disc (IVD) degeneration is the leading trigger of low back pain, which causes disability and leads to enormous healthcare toll worldwide. Biological treatment with growth factors has evolved as potential therapy for IVD regeneration. Bone morphogenetic protein 2 (BMP-2) and BMP-7 have shown promise in this regard. In the current study, we evaluated the effect of BMP-2/7 heterodimer for disc regeneration both in vitro and in organ culture. Nucleus pulposus (NP) cells isolated from bovine caudal disc were cultured in a fibrin-hyaluronan (FBG-HA) hydrogel for up to 14 days. BMP-2/7 heterodimer covalently incorporated within the hydrogel up-regulated the aggrecan and type II collagen gene expression, and glycosaminoglycan synthesis of NP cells. The activity of the BMP-2/7 heterodimer was dose dependent. The higher dose of BMP-2/7 was further assessed in an IVD whole organ system. After 14 days of culture with cyclic dynamic load, the BMP-2/7 heterodimer delivered into the nucleotomized region showed potential to stimulate the gene expression and synthesis of proteoglycan in the remaining NP tissue after partial nucleotomy. The gene expression level of type I collagen and alkaline phosphatase in the native disc tissue were not affected by BMP-2/7 treatment, indicating no adverse fibroblastic or osteogenic effect on the disc tissue. Intradiscal delivery of BMP-2/7 heterodimer may be a promising therapeutic approach for NP regeneration. The current IVD whole organ partial nucleotomy model may be utilized for screening of other biomaterials or drugs to treat early degenerative disc disorders. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res.


PMID: 27340938

DOI: 10.1002/jor.23351

Other references

Kozlov, L.A.; Voronin, K.V.; Poliakova, N.S., 1977: Specific features in reverse development of late toxemias and problems of early rehabilitation of parturients. Akusherstvo i Ginekologiia: 46-49

Koch, R., 1957: Rape or sudden death. Munchener Medizinische Wochenschrift 99(39): 1411-1413

Beliard, R.C.; Merrey, J.W., 2017: Evaluation of Interventions in Clinical Pharmacist-Led Annual Medicare Wellness Visits Compared with Usual Care. Evaluate and compare the number and types of medication and non-medication-related interventions by clinical pharmacists and nonpharmacists conducting an Annual Medicare Wellness Visit (MWV). Multi-center, retrospective case-control study. Two com...

Roth, Grace M., 1936: Hypersensitiveness to cold with local and systemic manifestations of a histamine-like character its amenability to treatment. 22 patients 15-59 yrs. of age, most of them apparently in good health were hypersensitive to cold air, water or even cold food as ice cream. Local reactions were present in all, systemic reactions as a syncope, even unconsciousness occurred in 14....

Ferguson, P.L.; Iden, C.R.; Brownawell, B.J., 2000: Analysis of alkylphenol ethoxylate metabolites in the aquatic environment using liquid chromatography-electrospray mass spectrometry. A quantitative method is described for the analysis of the metabolites of alkylphenol ethoxylate (APEO) surfactants in estuarine water and sediment samples using reversed-phase high-performance liquid chromatography with electrospray mass spectrom...

A.T.wari; S.J.a; R.P.l; S.S.thi; L.K.ishan, 2013: Effect of Pre-Milling Treatments on Storage Stability of Pearl Millet Flour. This work was undertaken to evaluate the effect of pre-milling treatments viz pearling, heat treatment and fermentation on pearl millet flour quality during storage. The pre-treatments yielded in significant reduction in antinutritional factors (p...

Merefield J.R., 1978: Trace elements as indicators of sediment transport in some estuaries of South-west England. Proceedings of the Ussher Society 4, Part 2: 219

Mead, A.; Little, H.J., 1993: Baclofen increases handling-induced behaviour in vivo. Alcoholism Clinical & Experimental Research 17(2): 504

Magid, A.Alabdul.; Bobichon, Hélène.; Borie, N.; Lalun, N.; Long, C.; Moretti, C.; Lavaud, C., 2010: Cytotoxic triterpenoid saponins from the stem bark of Antonia ovata. Phytochemical investigation of the MeOH extract of the stem bark of <i>Antonia ovata</i> led to the isolation of four triterpenoid saponins, along with eleven known compounds. Their structures were established by extensive 1D and 2D NM...

Abenhaim, H.A.; Azoulay, L.; Kramer, M.S.; Leduc, L., 2008: Incidence and risk factors of amniotic fluid embolisms: a population-based study on 3 million births in the United States. OBJECTIVE: Amniotic fluid embolism (AFE) is a condition occurring during delivery that can lead to severe maternal morbidity and mortality. Given the rarity of its occurrence, current estimates and predictors of the incidence and outcomes are oft...