geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

High sensitivity, high surface area Enzyme-linked Immunosorbent Assay (ELISA)


, : High sensitivity, high surface area Enzyme-linked Immunosorbent Assay (ELISA). Bio-Medical Materials and Engineering 26(3-4): 115-127

Enzyme-linked immunosorbent assays (ELISA) are considered the gold standard in the demonstration of various immunological reactions with an application in the detection of infectious diseases such as during outbreaks or in patient care. This study aimed to produce an ELISA-based diagnostic with an increased sensitivity of detection compared to the standard 96-well method in the immunologic diagnosis of infectious diseases. A '3DStack' was developed using readily available, low cost fabrication technologies namely nanoimprinting and press stamping with an increased surface area of 4 to 6 times more compared to 96-well plates. This was achieved by stacking multiple nanoimprinted polymer sheets. The flow of analytes between the sheets was enhanced by rotating the 3DStack and confirmed by Finite-Element (FE) simulation. An Immunoglobulin G (IgG) ELISA for the detection of antibodies in human serum raised against Rubella virus was performed for validation. An improved sensitivity of up to 1.9 folds higher was observed using the 3DStack compared to the standard method. The increased surface area of the 3DStack developed using nanoimprinting and press stamping technologies, and the flow pattern between sheets generated by rotating the 3DStack were potential contributors to a more sensitive ELISA-based diagnostic device.

US$19.90

PMID: 26684884

DOI: 10.3233/BME-151561


Other references

Klevjer-Anderson, P.; Anderson, L.W., 1982: Caprine arthritis-encephalitis virus infection of caprine monocytes. Monocyte-enriched cultures of goat peripheral blood leukocytes were exposed to caprine arthritis-encephalitis virus (CAEV) at multiplicity of infection of 8 TCID50 [median tissue culture infective dose]/cell as measured in goat synovial membrane c...

Aryavand, A., 1976: Some abnormal phenomena in pollen mitosis in astragalus candolleanus. Pollen mitosis occurs in the tetrads when the callosic envelope is still clearly distinct. It did not occur simultaneously in the different nuclei of the same tetrad, but was often asynchronous. This unusually early mitosis facilitated the demonst...

Rebaudo, R.; Melani, R.; Balestrino, M.; Izvarina, N., 2001: Electrophysiological effects of sustained delivery of CRF and its receptor agonists in hippocampal slices. The corticotropin-releasing factor (CRF) is a hypothalamic peptide that regulates the release of adrenocorticotropic hormone (ATCH) and of beta-endorphin. It has been suggested that it modulates learning and memory processes in rat. However, the e...

Eastman, T.R., 2012: Exposure technique documentation. Radiologic Technology 83(2): 202-203

Meister, Jörg.; Franzen, Ré.; Apel, C.; Gutknecht, N., 2004: Influence of the spatial beam profile on hard tissue ablation, part II: pulse energy and energy density distribution in simple beams. When calculating applied flux densities in practice, the beam profile of a laser is often erroneously assumed to be homogeneous. In addition, there is usually no consistency in the choice of a suitable measuring method for determining the beam dia...

Bednarek, A.; Chudek, J.; Karwasiecka, Dła.; Kubeczko, M.; Wojnar, J., 2016: Vitamin D deficiency among women diagnosed with breast cancer and unclear benefits of vitamin supplementation. Breast cancer is the most common cancer in the world and also in Poland. Morbidity for breast cancer is increasing, but mortality rate is still on the same level. In Poland morbidity has increased almost two times during the last 30 years. Vitamin...

Mack, A.D., 1946: Sintered Glass Disks. Science 103(2662): 21-22

Moriyama, Y.; Iguro, Y.; Yotsumoto, G.; Watanabe, S.; Yamamoto, H., 2000: Endovascular stent-graft repair with a flexible stent-graft and a simple applicator prepared from a syringe. Journal of Thoracic and Cardiovascular Surgery 119(3): 620-621

Wanek, V.; Born, J.; Novak, P.; Reime, B., 1999: Attitudes and health status as determinants of participation in individually oriented health promotion. In a cross-sectional health survey among employees of a large German metal company with 974 study participants, attitudes and aspects of the individual health status were identified which predispose employees to take part in individual health prom...

Madsen B.C.; Doller D.W., 1987: Continuous flow real time and flow injection determination of rainwater ph with a polyvinyl chloride based tubular membrane electrode. A continuous-flow method that utilizes a poly(vinyl chloride)-based tubular membrane electrode is used to determine the pH of rainwater in real time. A flow injection method based on the same tubular membrane electrode is applied to measurement of...