+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Identification of a conserved and acute neurodegeneration-specific microglial transcriptome in the zebrafish

, : Identification of a conserved and acute neurodegeneration-specific microglial transcriptome in the zebrafish. Glia 65(1): 138-149

NlmCategory="UNASSIGNED">Microglia are brain resident macrophages important for brain development, connectivity, homeostasis and disease. However, it is still largely unclear how microglia functions and their identity are regulated at the molecular level. Although recent transcriptomic studies have identified genes specifically expressed in microglia, the function of most of these genes in microglia is still unknown. Here, we performed RNA sequencing on microglia acutely isolated from healthy and neurodegenerative zebrafish brains. We found that a large fraction of the mouse microglial signature is conserved in the zebrafish, corroborating the use of zebrafish to help understand microglial genetics in mammals in addition to studying basic microglia biology. Second, our transcriptome analysis of microglia following neuronal ablation suggested primarily a proliferative response of microglia, which we confirmed by immunohistochemistry and in vivo imaging. Together with the recent improvements in genome editing technology in zebrafish, these data offer opportunities to facilitate functional genetic research on microglia in vivo in the healthy as well as in the diseased brain. GLIA 2016;65:138-149.


PMID: 27757989

DOI: 10.1002/glia.23083

Other references

Jüttler, E.; Fiebach, J.B.; Schellinger, P.D., 2005: Diagnostic imaging for acute ischemic stroke management. Although our clinical understanding remains our most important diagnostic tool, acute stroke therapy without neuroimaging is impossible. In most patients, only non-contrast computed tomography is used for diagnosis of acute stroke. However, findin...

Dealteris, J.T.; Morse, D.L., 1997: Fishing gear management. Boreman, J [Editor], Nakashima, B S [Editor], Wilson, J A [Editor], Kendall, R L [Editor] Northwest Atlantic groundfish: Perspectives on a fishery collapse 167-175

Monasterio, M.; Longsworth, K.A.; Viegas, S., 2016: Use of a bivalve finger fracture orthosis for a new treatment protocol of a PIP comminuted fracture and dorsal dislocation. As therapists and physicians, we often need to work with our patients to cater treatment accordingly. These authors describe how they modified a treatment protocol for a patient that sustained a proximal interphalangeal joint comminuted fracture a...

S.L.ansheng; Lin Jinshi, 1990: Study of the relationship between the short-period variation of geomagnetic field and b-value anomalies. Dizhen Yanjiu = Journal of Seismological Research 13(2): 181-188

Roberts, M.E.; Crail, J.P.; Laffoon, M.M.; Fernandez, W.G.; Menze, M.A.; Konkle, M.E., 2014: Identification of disulfide bond formation between MitoNEET and glutamate dehydrogenase 1. MitoNEET is a protein that was identified as a drug target for diabetes, but its cellular function as well as its role in diabetes remains elusive. Protein pull-down experiments identified glutamate dehydrogenase 1 (GDH1) as a potential binding pa...

Bartolozzi, G.; Bernini, G.; Maggini, M., 1975: Hypertrophic osteoarthropathy without pachydermia. Idiopathic form. A 3-year-old boy had primary hypertrophic osteoarthropathy without pachydermia. This case is similar to several others previously described, and it could possibly represent a distinct nosological form of osteoarthropathy.

Li, M.; Tamate, H.B.; Wei, F.W.n; Wang,; Masuda, R.; Sheng, H.L.n; Ohtaishi, N., 2003: Phylogenetic relationships among deer in China derived from mitochondrial DNA cytochrome b sequences. The phylogenetic relationships of Cervidae and Moschidae were examined using partial sequence data of mitochondrial DNA (mtDNA) cytochrome b. Ten new sequences were obtained for six species of Cervidae and Moschidae, and aligned with those previou...

Wei, W.; Faria, L.C.utinho; Mody, I., 2004: Low Ethanol Concentrations Selectively Augment the Tonic Inhibition Mediated by Subunit-Containing GABAA Receptors in Hippocampal Neurons. Journal of Neuroscience 24(38): 79-82

Ladduwahetty, T.; Keown, L.; Cascieri, M.A.; Sadowski, S., 1994: Morpholine-based substance P antagonists: Assessment of the 3-point binding model. The carbon-linked morpholine analogues of the Substance P antagonist CP-99,994 were synthesized. The activities of the resulting compounds suggest that there is a specific interaction between the benzylic heteroatom of these antagonists and the NK...

Chapman, S.; Woodward, S., 1991: Australian court rules that passive smoking causes lung cancer, asthma attacks, and respiratory disease. Bmj 302(6782): 943-945