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Identification of miRNAs involved in fruit ripening in Cavendish bananas by deep sequencing


, : Identification of miRNAs involved in fruit ripening in Cavendish bananas by deep sequencing. Bmc Genomics 16(): 776-776

MicroRNAs (miRNAs) are a family of non-coding small RNAs that play an important regulatory role in various biological processes. Previous studies have reported that miRNAs are closely related to the ripening process in model plants. However, the miRNAs that are closely involved in the banana fruit ripening process remain unknown. Here, we investigated the miRNA populations from banana fruits in response to ethylene or 1-MCP treatment using a deep sequencing approach and bioinformatics analysis combined with quantitative RT-PCR validation. A total of 125 known miRNAs and 26 novel miRNAs were identified from three libraries. MiRNA profiling of bananas in response to ethylene treatment compared with 1-MCP treatment showed differential expression of 82 miRNAs. Furthermore, the differentially expressed miRNAs were predicted to target a total of 815 target genes. Interestingly, some targets were annotated as transcription factors and other functional proteins closely involved in the development and the ripening process in other plant species. Analysis by qRT-PCR validated the contrasting expression patterns between several miRNAs and their target genes. The miRNAome of the banana fruit in response to ethylene or 1-MCP treatment were identified by high-throughput sequencing. A total of 82 differentially expressed miRNAs were found to be closely associated with the ripening process. The miRNA target genes encode transcription factors and other functional proteins, including SPL, APETALA2, EIN3, E3 ubiquitin ligase, β-galactosidase, and β-glucosidase. These findings provide valuable information for further functional research of the miRNAs involved in banana fruit ripening.

US$19.90

PMID: 26462563

DOI: 10.1186/s12864-015-1995-1


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Lee, J-Der.; Chu, Y-Hsuan.; Chen, C-Wei.; Lin, K-Ju., 2010: Multi-image registration for evaluating the 99mTc-TRODAT-1 of Parkinson's rat model. Study on small animal model using multiple imaging is an important procedure in medicine and physiology. Recently, Single Photon Emission Computed Tomography (SPECT) of dopamine transporter with (99cm)Tc-TRODA-1 has been proved to be a valuable an...

Zhang, C.; Malhotra, S.V.; Francis, A.J., 2011: Toxicity of imidazolium- and pyridinium-based ionic liquids and the co-metabolic degradation of N-ethylpyridinium tetrafluoroborate. We examined the effects of the ionic liquids (ILs), 1-butyl-3-methylimidazolium hexafluorophosphate [BMIM][PF₆], N-ethylpyridinium tetrafluoroborate [EtPy][BF₄], and N-ethylpyridinium trifluoroacetate [EtPy][CF₃COO] on Pseudomonas fluorescen...

Anonymous, 2012: Gene links rare and unrelated cancers. Scientists at the BC Cancer Agency, Vancouver Coastal Health Research Institute, and the University of British Columbia are excited over a discovery made while studying rare tumor types.

Porter, K.B.; Tsibris, J.C.; Nicosia, S.V.; Murphy, J.M.; O'Brien, W.F.; Rao, P.S.; Spellacy, W.N., 1995: Estrogen-induced guinea pig model for uterine leiomyomas: do the ovaries protect?. A guinea pig model was used to study the hormonal control of uterine leiomyomas. Twenty female guinea pigs were divided into four groups-young, old, ovariectomized (OVX), and non-OVX animals-and were given two estradiol-17-beta (E-2) silastic impl...

Pane, B.; Spinella, G.; Perfumo, C.; Palombo, D., 2016: A Single-Center Experience of Aortic and Iliac Artery Aneurysm Treated with Multilayer Flow Modulator. The aim of this study was to analyze our series of endovascular treatments using a multilayer flow modulator (MFM) and to show the midterm results. At our institution, 8 patients were treated with an MFM. Four patients presented with an aortic ane...

Loher W.; Chandrashekaran M.K., 1972: Communicative behavior of the grasshopper syrbula fuscovittavata gomphocerinae with particular consideration of the male courtship. Zeitschrift fuer Tierpsychologie 31(1): 78-97

Jenks, C.; Smith, J.A., 2008: After Beslan: childhood, complexity and risk. This paper addresses the events at Beslan as a crisis point at which the postmodern celebration of difference spills into unbearable chaos. However this chaos turns out to show specific, dynamic or complex, self-organizing structures. Such dynamic...

Anonymous, 1951: Proceedings of the 8th Annual Conference of the Indian Tea Association, Tocklai, December 1950. The conference was addresssd on, and discussed, the following subjects: Recent experiments in tea manufacture, the present position with regard to modernization and mechanization of the tea industry both in the field and in the factory with partic...

Yamamoto I.; Bringhurst F.R.; Potts J.T.Jr; Segre G.V., 1982: Regulation of parathyroid hormone binding to clonal rat osteo sarcoma cells. Calcified Tissue International 34(SUPPL 1): S47

Luch, A.; Schober, W.; Soballa, V.J.; Raab, G.; Greim, H.; Jacob, J.; Doehmer, J.; Seidel, A., 1999: Metabolic activation of dibenzo[a,l]pyrene by human cytochrome P450 1A1 and P450 1B1 expressed in V79 Chinese hamster cells. Metabolic activation of the strongly carcinogenic polycyclic aromatic hydrocarbon (PAH) dibenzo[a,l]pyrene (DB[a,l]P) and its trans-8,9-dihydrodiol (trans-8,9-diol) catalyzed by human cytochromes P450 (P450) 1A1 and 1B1 was investigated. DNA bindi...