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Improved serological detection of rheumatoid arthritis: a highly antigenic mimotope of carbonic anhydrase III selected in a murine model by phage display

, : Improved serological detection of rheumatoid arthritis: a highly antigenic mimotope of carbonic anhydrase III selected in a murine model by phage display. Arthritis Research & Therapy 17(): 168-168

Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease that affects around 1% of the human population worldwide. RA diagnosis can be difficult as there is no definitive test for its detection. Therefore, the aim of this study was to identify biomarkers that could be used for RA diagnosis. Sera from a collagen-induced arthritis mouse model were used to select potential biomarkers for RA diagnosis by phage display technology. In silico and in vitro analyses were performed to characterize and validate the selected peptides. Samples were classified into three groups: RA; two other immune-mediated rheumatic diseases (systemic lupus erythematosus (SLE) and ankylosing spondylitis (AS)); and healthy controls (HC). Enzyme-linked immunosorbent assay (ELISA) was carried out to determine antibody levels, and diagnostic parameters were determined by constructing receiver operating characteristic curves. Mass spectrometry and Western blot were performed to identify the putative autoantigen that was mimicked by a highly reactive mimotope. After three rounds of selection, 14 clones were obtained and tested for immunoreactivity analysis against sera from RA and HC groups. The phage-fused peptide with the highest immunoreactivity (M12) was synthesized, and was able to efficiently discriminate RA patients from SLE, AS and HCs (p < 0.0001) by ELISA. The specificity and sensitivity of anti-M12 antibodies for RA diagnosis were 91 % and 84.3 %, respectively. The M12 peptide was identified as one that mimics a predicted antigenic site of the carbonic anhydrase III (CAIII) protein, a ubiquitous biomarker that has been identified in patients with other diseases. M12 is the first peptide associated with the CAIII protein that may be used as an antigen for antibody detection to aid in RA diagnosis with high sensitivity and specificity.


PMID: 26099944

DOI: 10.1186/s13075-015-0685-3

Other references

Wada, N.; Kanda, Y.; Takayama, R., 1998: Neuronal pathways from foot pad afferents to hindlimb motoneurons in the low spinalized cats. Experiments were performed on 16 adult spinalized (L2) cats. Postsynaptic potentials (PSPs) produced by electrical stimulation of afferent nerves innervating foot pads were recorded from hindlimb motoneurons innervating the following hindlimb musc...

Bansal, N.; Whooley, M.A.; Regan, M.; McCulloch, C.E.; Ix, J.H.; Epel, E.; Blackburn, E.; Lin, J.; Hsu, C-Yuan., 2013: Association between kidney function and telomere length: the heart and soul study. Telomere attrition is a novel risk factor for cardiovascular disease. Studies of telomere length in relation to kidney function are limited. We explored the association of kidney function with telomere length and telomere shortening. The Heart and...

Tucker, K.J.; Türker, K.S., 2005: Muscle spindle feedback differs between the soleus and gastrocnemius in humans. The Hoffmann (H) reflex and motor (M) response were studied in soleus and gastrocnemius during voluntary contraction in eight male volunteers. Aims: To determine if the strength of spindle input to the muscles is the same. To assess if the M respo...

Macey, P.E., 1962: The present status of antibiotics. Malcolm Woodbine, Editor: Iotics In Agriculture. Proceedings Of The University Of Nottingham Ninth Easter School In Agricultural Science. 1-20

Haasper, C.; Kalmbach, M.; Dikos, G.D.; Meller, R.; Müller, C.; Krettek, C.; Hildebrand, F.; Frink, M., 2010: Prognostic value of procalcitonin (PCT) and/or interleukin-6 (IL-6) plasma levels after multiple trauma for the development of multi organ dysfunction syndrome (MODS) or sepsis. Despite recent advances in treatment of severe injured patients, e.g. due to damage control orthopaedics, multi organ dysfunction syndrome (MODS) and sepsis are major complications in daily practice. During one year 94 patients were prospectively...

Anonymous, 1988: Painful clinical trials. Paediatric and Perinatal Epidemiology 2(1): 1-2

Hoven, B.N., 1990: Kristansand's nursing college. Matters in rehabilitation. Interview by Kjell Arne Bakke. Fag Tidsskriftet Sykepleien 78(6): 28-29

Melchior, E., 1975: 28 Feldmause als Vorrat in Mausebussardhorst Buteo buteo. Regulus, 1014: 402

Dien, J., 1964: Ornithologischer Jahresbericht 1963 fur das Hamburger Berichtsgebiet. Hamburger Avifaunistische Beitrage, 1: 115-158

Jiao, Y.; Zhang, Y.; Wei, Y.; Liu, Z.; An, W.; Guo, M., 2013: Direct observation of internalization and ROS generation of amyloid β-peptide in neuronal cells at subcellular resolution. Seeing in many colors: Confocal images acquired using fluorescently labeled amyloid β-peptide revealed its efficient internalization by endocytosis into endosomes/lysosomes of human neuronal cells with a small portion reaching mitochondria, induc...