geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

In Vivo Interleukin-13-Primed Macrophages Contribute to Reduced Alloantigen-Specific T Cell Activation and Prolong Immunological Survival of Allogeneic Mesenchymal Stem Cell Implants


, : In Vivo Interleukin-13-Primed Macrophages Contribute to Reduced Alloantigen-Specific T Cell Activation and Prolong Immunological Survival of Allogeneic Mesenchymal Stem Cell Implants. Stem Cells 34(7): 1971-1984

Transplantation of mesenchymal stem cells (MSCs) into injured or diseased tissue-for the in situ delivery of a wide variety of MSC-secreted therapeutic proteins-is an emerging approach for the modulation of the clinical course of several diseases and traumata. From an emergency point-of-view, allogeneic MSCs have numerous advantages over patient-specific autologous MSCs since "off-the-shelf" cell preparations could be readily available for instant therapeutic intervention following acute injury. Although we confirmed the in vitro immunomodulatory capacity of allogeneic MSCs on antigen-presenting cells with standard coculture experiments, allogeneic MSC grafts were irrevocably rejected by the host's immune system upon either intramuscular or intracerebral transplantation. In an attempt to modulate MSC allograft rejection in vivo, we transduced MSCs with an interleukin-13 (IL13)-expressing lentiviral vector. Our data clearly indicate that prolonged survival of IL13-expressing allogeneic MSC grafts in muscle tissue coincided with the induction of an alternatively activated macrophage phenotype in vivo and a reduced number of alloantigen-reactive IFNγ- and/or IL2-producing CD8(+) T cells compared to nonmodified allografts. Similarly, intracerebral IL13-expressing MSC allografts also exhibited prolonged survival and induction of an alternatively activated macrophage phenotype, although a peripheral T cell component was absent. In summary, this study demonstrates that both innate and adaptive immune responses are effectively modulated in vivo by locally secreted IL13, ultimately resulting in prolonged MSC allograft survival in both muscle and brain tissue. Stem Cells 2016;34:1971-1984.

US$19.90

PMID: 26992046

DOI: 10.1002/stem.2360


Other references

Kudrjavcev, P.N.; Lebedev, J. V., 1960: Measuring the depth of fat on the live pig. On the day before slaughter the depth of fat was measured with a marked probe at the shoulder, back and rump of 36 pigs with a live weight of 100 kg. The carcasses were measured at the thickest part of the shoulder, at the 6th-7th rib, at the firs...

Mullol, J.; Fernandez Morata, J.C.; Roca Ferrer, J.; Pujols, L.; Xaubet, A.; Benitez, P.; Picado, C., 2002: Cyclooxygenase 1 and cyclooxygenase 2 expression is abnormally regulated in human nasal polyps. Background: There is evidence that impairment of prostanoid metabolism might be involved in the pathogenesis of nasal polyps (NPs). Prostanoids are synthesized by 2 cyclooxygenase (Cox) enzymes, one constitutive (Cox-1) and another inducible (Cox-...

Westbroek, P.; Temmerman, E.; Kiekens, P.; Govaert, F., 2008: An automated system for the measurement of hydrogen peroxide in industrial applications. An automated sensor system for the continuous and in-line measurement of hydrogen peroxide in industrial applications is described. The hydrogen peroxide concentration can be measured over the entire pH range, over a wide concentration range of hy...

Anonymous, 2012: Clinical Manifestations and Distribution of Cutaneous Leishmaniasis in Pakistan. Cutaneous leishmaniasis (CL) is a rising epidemic in Pakistan. It is a major public health problem in the country especially alongside regions bordering the neighboring Afghanistan and cities that have had the maximum influx of refugees. The purpo...

Coyne, D.W., 2008: A comprehensive vision for intravenous iron therapy. Commonly used iron indices, such as serum ferritin and transferrin saturation (TSAT), have limited utility in patients with chronic kidney disease. Both dialysis and nondialysis patients may have normal to high serum ferritin levels and little or...

Das; Hott, 1996: Thermal effects on the catalysis by a magnetic field. Physical Review. D, Particles and Fields 53(4): 2252-2255

Marchese, M.A.; Saéz-Alquézar, A.; Pontes, J.F., 1978: Pepsinogen: biochemical and clinical aspects. Arquivos de Gastroenterologia 15(4): 180-182

Hayashi, T.; Ichimura, T.; Kasai, M.; Sano, K.; Zharhary, D.; Shiozawa, T.; Yaegashi, N.; Konishi, I., 2017: Characterization of Leiomyomatoid Angiomatous Neuroendocrine Tumour (LANT)-like Tumour in the Myometrium with Histopathological Examination. Leiomyomatoid angiomatous neuroendocrine tumour (LANT) is possibly a new disease entity that was reported as a dimorphic neurosecretory tumour with a leiomyomatous vascular component; it was found in the pituitary. We describe uterine LANT-like ma...

Anonymous, 2003: Toxicology and carcinogenesis studies of riddelliine (CAS No. 23246-96-0) in F344/N rats and B6C3F1 mice (gavage studies). Riddelliine belongs to a class of toxic pyrrolizidine alkaloids and is isolated from plants of the genera Crotalaria, Amsinckia, and Senecio that grow in the western United States. Cattle, horses, and sheep that ingest these plants succumb to thei...

Muralidharan A.; Balakrishnan S., 1972: Studies on the performance of some varieties of turmeric curcuma sp and its fertilizer requirements. Agricultural Research Journal of Kerala 10(2): 112-115