+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Inhibitory effect of divalent metal cations on zinc uptake via mouse Zrt-/Irt-like protein 8 (ZIP8)

, : Inhibitory effect of divalent metal cations on zinc uptake via mouse Zrt-/Irt-like protein 8 (ZIP8). Life Sciences (): -

There is controversy regarding the substrate specificity of ZIP8, a ZIP isoform, involved in regulation of extra- and intracellular zinc levels. Here, we investigated the inhibitory effects of divalent metal cations on zinc uptake via mouse ZIP8 (mZIP8). mZIP8 cDNA was transfected into HEK293T cells by a lipofection method, and its functional expression was evaluated by immunocytochemistry, Western blotting and 65Zn (65ZnCl2) uptake measurement. Transfection of mZIP8 cDNA into HEK293T cells induced expression of mZIP8 in the cells, and increased zinc uptake. mZIP8-mediated zinc uptake depended on extracellular bicarbonate, and the Michaelis constant for the uptake was estimated to be 8.48±2.46μM. In the inhibition study, iron and cadmium competitively, and cobalt, nickel and copper non-competitively inhibited the mZIP8-mediated zinc uptake, the inhibition constants being calculated to be 3.37, 55.5, 80.6, 198 and 48.3μM, respectively. In contrast, magnesium and manganese at concentrations of up to 1500 and 200μM, respectively, had no inhibitory effect on the zinc uptake via mZIP8. In this study, we reveal that the inhibition profiles of divalent metal cations as to zinc uptake via mZIP8 apparently differ from those for mZIP1, especially in the affinity and inhibition manner of nickel. These findings should contribute to identification of ZIP isoforms involved in total cellular zinc transport.


PMID: 27956349

DOI: 10.1016/j.lfs.2016.12.006

Other references

Fergusson, W., 1842: Clinical Lecture on Lithotomy. Provincial Medical Journal and Retrospect of the Medical Sciences 5(115): 213-216

Olsson, Gert, 1997: Movements, home ranges and habitat selection of mountain hares in a managed, boreal forest. Gibier Faune Sauvage 14(3): 522-523, Sept

Szuzuki, K.; Okamoto, T.; Yoshimura, Y.; Deyama, Y.; Hisada, Y.; Matsumoto, A., 1995: Phosphotyrosyl protein phosphatase-like activity of a clonal osteoblastic cell line (MC3T3-E1 cell). The homogenate of MC3T3-E1 cells hydrolysed phosphotyrosine, but not phosphoserine or phosphothreonine at acidic pH. It dephosphorylated lysozyme and Raytide (a gastrin analogue peptide) phosphorylated by tyrosine kinase, but showed little activit...

Phung, T.Xuan.Binh.; Tran, T.Hong.Hanh.; Dan, T.Thuy.Hang.; Chau, V.Minh.; Hoang, T.Huong.; Nguyen, T.Dat., 2012: Chalcone-derived Diels-Alder adducts as NF-κB inhibitors from Morus alba. A bioassay-guided phytochemical fractionation of the methanol extract of the Morus alba root barks led to the isolation of two chalcone-derived Diels-Alder adducts (1 and 2). Their structures were elucidated as kuwanon J 2,4,10″-trimethyl ether...

Brown B.R., 1977: Gippsland's old and new oil. The APEA Journal 17, Part 2: 47-57

Kamrava, M.; Kuske, R.R.; Anderson, B.; Chen, P.; Hayes, J.; Quiet, C.; Wang, P-Chieh.; Veruttipong, D.; Snyder, M.; Demanes, D.J., 2016: Outcomes of Node-positive Breast Cancer Patients Treated With Accelerated Partial Breast Irradiation Via Multicatheter Interstitial Brachytherapy: The Pooled Registry of Multicatheter Interstitial Sites (PROMIS) Experience. To report outcomes for breast-conserving therapy using adjuvant accelerated partial breast irradiation (APBI) with interstitial multicatheter brachytherapy in node-positive compared with node-negative patients. From 1992 to 2013, 1351 patients (13...

Vallejo, A.N., 2011: Immunological hurdles of ageing: indispensable research of the human model. Census reports of many countries indicate continuing trends for the graying of their populations. For the United States alone, persons aged ≥65 years are projected to comprise over 20% of the population by the year 2050. In view of the special m...

Singh, D.K.; Tiwari, D.P.; Saksena, D.N., 1993: Removal of lead from aqueous solutions by chemically treated used tea leaves. Used tea leaves powder treated with formaldehyde and sulphuric acid has been studied as a sorbent for removal of Pb-2+ from aqueous solutions. Effect of contact time, initial concentration, pH and temperature has been studied. Sorption capacities...

Storr, P., 1968: Admission of practice possession contracts and competition paragraphs contained therein. Therapie der Gegenwart 107(10): 1338 Passim-1338 Passim

Stankovic, A.K.; Waites, K.B., 1997: Comparison of the MicroScan WalkAway 40 and the Vitek System for antimicrobial susceptibility testing. Abstracts of the General Meeting of the American Society for Microbiology 97(0): 205