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Inter- and intra-patient clonal and subclonal heterogeneity of chronic lymphocytic leukaemia: evidences from circulating and lymph nodal compartments

, : Inter- and intra-patient clonal and subclonal heterogeneity of chronic lymphocytic leukaemia: evidences from circulating and lymph nodal compartments. British Journal of Haematology 172(3): 371-383

Whole exome sequencing and copy number aberration (CNA) analysis were performed on cells taken from peripheral blood (PB) and lymph nodes (LN) of patients with chronic lymphocytic leukaemia (CLL). Of 64 non-silent somatic mutations, 54 (84·4%) were clonal in both compartments, 3 (4·7%) were PB-specific and 7 (10·9%) were LN-specific. Most of the LN- or PB-specific mutations were subclonal in the other corresponding compartment (variant frequency 0·5-5·3%). Of 41 CNAs, 27 (65·8%) were shared by both compartments and 7 (17·1%) were LN- or PB-specific. Overall, 6 of 9 cases (66·7%) showed genomic differences between the compartments. At subsequent relapse, Case 10, with 6 LN-specific lesions, and Case 100, with 6 LN-specific and 8 PB-specific lesions, showed, in the PB, the clonal expansion of LN-derived lesions with an adverse impact: SF3B1 mutation, BIRC3 deletion, del8(p23·3-p11·1), del9(p24·3-p13·1) and gain 2(p25·3-p14). CLL shows an intra-patient clonal heterogeneity according to the disease compartment, with both LN and PB-specific mutations/CNAs. The LN microenvironment might contribute to the clonal selection of unfavourable lesions, as LN-derived mutations/CNAs can appear in the PB at relapse.


PMID: 26597680

DOI: 10.1111/bjh.13859

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Zheng, C-Jian.; Huang, B-Kang.; Wang, Y.; Ye, Q.; Han, T.; Zhang, Q-Yan.; Zhang, H.; Qin, L-Ping., 2010: Anti-inflammatory diterpenes from the seeds of Vitex negundo. Phytochemical investigation of a dichloromethane-soluble extract of Vitexnegundo seeds led to the isolation of five labdane diterpenes, negundoins A-E (1-5), a 9,10-seco-abietane diterpene, negundoin F (6), a sandaracopimara-7,15-diene diterpene,...

Stempfle, N.; Huten, Y.; Fredouille, C.; Brisse, H.; Nessmann, C., 1999: Skeletal abnormalities in fetuses with Down's syndrome: a radiographic post-mortem study. To evaluate skeletal abnormalities on post-mortem radiographs of fetuses with Down's syndrome. Biometrical and morphological criteria, which are used for US prenatal detection of trisomy 21, were assessed. Limb long bones, biparietal diameter...

Carroll, S., 2001: The Do Not Resuscitate order. Anaesthesia 56(1): 86-86

Pilas Pérez, M.; Ugalde Diez, M.; Alberquilla Menéndez-Asenjo, A.; González Rodríguez-Salinas, M.C., 1996: Characteristics of the requests in primary care facilities. Revista de Enfermeria 19(212): 26-32

Markosyan, R.A.; Popov, E.G.; Gabbasov, Z.A.; Pozin EYa, 1986: Effect of alternating pressure on thrombin-stimulated platelets. The effect of alternating pressure on several basic physiological processes of thrombin-stimulated platelets, e.g. shape changes, release of adenine nucleotides and activation of arachidonic platelet system was studied. It is assumed that redistri...

Montague, M.; van Garderen, D., 2004: A cross-sectional study of mathematics achievement, estimation skills, and academic self-perception in students of varying ability. This study investigated students' mathematics achievement, estimation ability, use of estimation strategies, and academic self-perception. Students with learning disabilities (LD), average achievers, and intellectually gifted students (N = 13...

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Garcia E.S.; Uhl M.; Rembold H., 1986: Azadirachtin a chemical probe for the study of molting processes in rhodnius prolixus. The effects of azadirachtin A after injection into timed 4th-instar nymphs of Rhodnius prolixus were studied. A dose-response relationship of Aza A was established using moulting inhibition and mortality as effective parameters. Aza A, if injected...

Glazer, H.I., 1972: Physostigmine and resistance to extinction. In order to study the relationship between pharmacologically induced hippocampal theta rhythm and resistance to extinction of instrumental responding, three groups of rats were trained on a discrete-trial FR 10 schedule of reinforcement, one group...

Romanowicz, R.; Beven, K., 1998: Dynamic real-time prediction of flood inundation probabilities. The Bayesian Generalised Likelihood Uncertainty Estimation (GLUE) methodology, previously used in rainfall-runoff modelling, was applied to the distributed problem of predicting the space and time varying probabilities of inundation of all points...