+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Interaction model of steviol glycosides from Stevia rebaudiana (Bertoni) with sweet taste receptors: A computational approach

, : Interaction model of steviol glycosides from Stevia rebaudiana (Bertoni) with sweet taste receptors: A computational approach. Phytochemistry 116: 12-20

Docking studies were performed on natural sweeteners from Stevia rebaudiana by constructing homology models of T1R2 and T1R3 subunits of human sweet taste receptors. Ramachandran plot, PROCHECK results and ERRAT overall quality factor were used to validate the quality of models. Furthermore, docking results of steviol glycosides (SG's) were correlated significantly with data available in the literature which enabled to predict the exact sweetness rank order of SG's. The binding pattern indicated that Asn 44, Ans 52, Ala 345, Pro 343, Ile 352, Gly 346, Gly 47, Ala 354, Ser 336, Thr 326 and Ser 329 are the main interacting amino acid residues in case of T1R2 and Arg 56, Glu 105, Asp 215, Asp 216, Glu 148, Asp 258, Lys 255, Ser 104, Glu 217, Leu 51, Arg 52 for T1R3, respectively. Amino acids interact with SG's mainly by forming hydrogen bonds with the hydroxyl group of glucose moieties. Significant variation in docked poses of all the SG's were found. In this study, we have proposed the mechanism of the sweetness of the SG's in the form of multiple point stimulation model by considering the diverse binding patterns of various SG's, as well as their structural features. It will give further insight in understanding the differences in the quality of taste and will be used to improve the taste of SG's using semi-synthetic approaches.


PMID: 26021732

DOI: 10.1016/j.phytochem.2015.05.006

Other references

Riggs Stanley; Stille Peter; Clauer Norbert, 1993: Sr isotopic analysis of phosphate peloids through multiple sea level cycles; testing the depositional model for the North Carolina continental margin. Special Publication - Florida Geological Survey Pages 107-109

Hanus, J., 1987: Study of chromium content and its depletion from the soil by new winter wheat cultivars. The chromium content of 4 varieties was spectrophotometrically determined in the above-ground organs at flowering and in the grain and straw at complete maturity. The depletion of chromium from the soil during the formation of grain and straw yiel...

Anonymous, 2013: Type VII collagen associated with the basement membrane of amniotic epithelium forms giant anchoring rivets which penetrate aA massive lamina reticularis. In human amnion a simple cuboidal epithelium and underlying fibroblast layer are separated by an almost acellular <italic>compact layer</italic> rich in collagen types I and III. This (&gt;10 m) layer, which may be a thick lamina r...

Liedtke, C.M., 1995: The role of protein kinase C in alpha-adrenergic regulation of NaCl(K) cotransport in human airway epithelial cells. alpha-1-Adrenergic (alpha-1-AR) agents stimulate NaCl(K) cotransport and phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P-2)-specific phospholipase C in human trachea and nasal polyp epithelial cells. One second messenger generated by PtdIns(4,...

Edgett Ken, 1997: Mars Pathfinder, Sojourner, and a cast of rock stars captivate earthlings. Earth in Space: , Pages 5-10. 1997.

Chang, J.C.; Dado, D.; Schussler, S.; Hawker, L.; Holland, C.L.; Burke, J.G.; Cluss, P.A., 2012: In person versus computer screening for intimate partner violence among pregnant patients. To compare in person versus computerized screening for intimate partner violence (IPV) in a hospital-based prenatal clinic and explore women's assessment of the screening methods. We compared patient IPV disclosures on a computerized question...

Ghosh, P.S.; Sorenson, E.J., 2016: Use of Clinical and Electrical Myotonia to Differentiate Childhood Myopathies. We retrospectively reviewed 2030 childhood electromyograms performed over an 11-year period (2004-2014). Twenty children (1%) with myotonic discharges were identified and placed into 2 groups. Group A (electrical and clinical myotonia) comprised 9...

Butler, L.M.; Metson-Scott, T.; Felix, J.; Abhyankar, A.; Rainger, G.Ed.; Farndale, R.W.; Watson, S.P.; Nash, G.B., 2007: Sequential adhesion of platelets and leukocytes from flowing whole blood onto a collagen-coated surface: requirement for a GpVI-binding site in collagen. The adhesion of leukocytes to immobilised platelets may contribute to inflammatory and thrombotic responses in damaged tissue. To investigate the conditions under which platelets and leukocytes might be deposited together in vessels, we perfused...

Yoshinari, T., 1976: Nitrous oxide in the sea. A new gas chromatographic method was developed for the measurement of N2O in seawater. It takes 15 min for the analysis of a sample of 640 ml. By this method, more than 1000 samples taken over various depths and areas in the sea were analyzed. In...

D.Muylder, C., 1954: Controversial questions with reference to lung cancer. Journal Belge de Radiologie 37(3): 243-270