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Intracellular labeling of mouse embryonic stem cell-derived neural progenitor aggregates with micron-sized particles of iron oxide


, : Intracellular labeling of mouse embryonic stem cell-derived neural progenitor aggregates with micron-sized particles of iron oxide. CytoTherapy 17(1): 98-111

Pluripotent stem cell (PSC)-derived neural progenitor cells (NPCs) represent an unlimited source for the treatment of various neurological disorders. NPCs are usually derived from PSCs through the formation of embryoid body (EB), an aggregate structure mimicking embryonic development. This study investigated the effect of labeling multicellular EB-NPC aggregates with micron-sized particles of iron oxide (MPIO) for cell tracking using magnetic resonance imaging (MRI). Intact and dissociated EB-NPC aggregates were labeled with various concentrations of MPIOs (0, 2.5, 5 and 10 μg Fe/mL). The labeled cells were analyzed by fluorescent imaging, flow cytometry and in vitro MRI for labeling efficiency and detectability. Moreover, the biological effects of intracellular MPIO on cell viability, cytotoxicity, proliferation and neural differentiation were evaluated. Intact EB-NPC aggregates showed higher cell proliferation and viability compared with the dissociated cells. Despite diffusion limitation at low MPIO concentration, higher concentration of MPIO (i.e., 10 μg Fe/mL) was able to label EB-NPC aggregates at similar efficiency to the single cells. In vitro MRI showed concentration-dependent MPIO detection in EB-NPCs over 2.0-2.6 population doublings. More important, MPIO incorporation did not affect the proliferation and neural differentiation of EB-NPCs. Multicellular EB-NPC aggregates can be efficiently labeled and tracked with MPIO while maintaining cell proliferation, phenotype and neural differentiation potential. This study demonstrated the feasibility of labeling EB-NPC aggregates with MPIO for cellular monitoring of in vitro cultures and in vivo transplantation.

US$19.90

PMID: 25527864

DOI: 10.1016/j.jcyt.2014.09.008


Other references

Kinoshita Hajimu; Fujisawa Hideyuki, 1973: Measurements of ultrasonic wave velocities of solids under high pressures and high temperatures; supplement to studies on seismo-magnetic phenomena. Pages 26-28 1973

Han, H.-Song; Yang, S.-Ling; Yeh, H.-Yi; Lin, J.-Che; Wu, H.-Lin; Shi, G.-Yueh, 2001: Studies of a novel human thrombomodulin immobilized substrate: Surface characterization and anticoagulation activity evaluation. Immobilization of the anticoagulative or antithrombogenic biomolecule has been considered as one of the important methods to improve the blood compatibility of artificial biomaterials. In this study, a novel immobilization reaction scheme was util...

Bayot, D., 1981: Low dose oral progestogens in human fertility control. Low-dose progestin only contraceptive agents seem to respect the hypothalamo-hypophyso-ovarian system, thus respecting the physiology of the menstrual cycle, and to inhibit fertility simply by interfering with estrogen action at the level of the c...

Fang, W.H., 1980: Aprindine for intractable dysarrhythmia. Zhonghua Xin Xue Guan Bing Za Zhi 8(3): 210-212

Elleder, M.; Ledvinová, J.; Cieslar, P.; Kuhn, R., 1990: Subclinical course of cholesterol ester storage disease (CESD) diagnosed in adulthood. Report on two cases with remarks on the nature of the liver storage process. An extremely benign variant of cholesterol ester storage disease (CESD) was diagnosed in two female patients aged 43 and 56 years. In one of them the course was entirely subclinical until a stroke at the age of 47, most probably a complication of...

Bhandari, H.S.; Thapa, B.; Acharya, G.P.; Palikhe, M.M.; Prasad, R.B.; Karki, T.B.; Upadhyay, K.P.; Ghimire, K.H., 2000: Increasing wheat productivity through genetic improvement and management research (1999/2000). Twelve crosses were made in 1999-2000 in Nepal between rust resistant and high yielding wheat genotypes. A coordinated variety trial was conducted to evaluate wheat germplasm for yield potential, cold tolerance and resistance to cold-induced steri...

Tan, B.H.; Tam, K.C.; Dupin, D.; Armes, S.P., 2009: Rheological behavior of acid-swellable cationic copolymer latexes. 2-Vinylpyridine (2VP) was copolymerized with four different cross-linker densities ranging from 0.05 to 0.31 wt % divinylbenzene (DVB) via aqueous emulsion polymerization to produce a series of submicrometer-sized, lightly cross-linked P2VP latexe...

Manoharachary, C.; Reddy, P.J.gan Mohan; Prabhakar, B.; Mohan, K.C.andra, 1997: Fungal spora and biodeterioration in some museums and libraries of Hyderabad, India. The fungal spores found inhabiting the museum and libraries were collected, isolated and identified employing standard air sampling and microbiological methods. Altogether 49 fungal spores were identified. Sources of fungal inoculum were also iden...

Johnson, E.A.; Miyanishi, K.; O'Brien, N., 1999: Long-term reconstruction of the fire season in the mixedwood boreal forest of Western Canada. Climate modelling studies have predicted an increase in fire frequency with global warming as well as suggesting a longer fire season occurring later in the year. Fire scars for 160 years (1831-1948) for Pinus banksiana, and written fire records f...

Dobre, A., 1979: When it is easy in the building of forest roads, do we leave birch stands as a natural beautification?. Sodobno kmetijstvo 12(2): 91-93