+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Investigation of Cell Viability and Morphology in 3D Bio-printed Alginate Constructs with Tunable Stiffness

, : Investigation of Cell Viability and Morphology in 3D Bio-printed Alginate Constructs with Tunable Stiffness. Journal of Biomedical Materials Research. Part A (): -

NlmCategory="UNASSIGNED">In this article, mouse fibroblast cells (L929) were seeded on 2%, 5% and 10% alginate hydrogels, and they were also bio-printed with 2%, 5% and 10% alginate solutions individually to form constructs. The elastic and viscous moduli of alginate solutions, their interior structure and stiffness, interactions of cells and alginate, cell viability, migration and morphology were investigated by rheometer, MTT assay, scanning electron microscope (SEM) and fluorescent microscopy etc. The 3 types of bio-printed scaffolds of distinctive stiffness were prepared, and the seeded cells showed robust viability either on the alginate hydrogel surfaces or in the 3D bio-printed constructs. Majority of the proliferated cells in the 3D bio-printed constructs weakly attached to the surrounding alginate matrix. The concentration of alginate solution and hydrogel stiffness influenced cell migration and morphology, moreover the cells formed spheroids in the bio-printed 10% alginate hydrogel construct. This article is protected by copyright. All rights reserved.


PMID: 27935198

DOI: 10.1002/jbm.a.35971

Other references

Smith, R.J., 1979: Congress says bioassay reports are stalled. Science 204(4399): 1288-1288

Arrer, E.; Gibitz, H.J., 1987: Detection of beta 2-transferrin with agarose gel electrophoresis, immunofixation and silver staining in cerebrospinal fluid, secretions and other body fluids. A method is described for the detection of beta 2-transferrin (asialotransferrin) in 1 microliter cerebrospinal fluid. Owing to its low content of sialic acid, beta 2-transferrin migrates more slowly than the main transferrin component (beta 1-tra...

Igo, M.M.; Losick, R., 1986: Regulation of a promoter that is utilized by minor forms of RNA polymerase holoenzyme in Bacillus subtilis. The ctc gene of Bacillus subtilis is transcribed in vitro by the minor RNA polymerase holoenzyme forms, E sigma 37 and E sigma 32. To study the expression and regulation of ctc in vivo, we constructed operon and translational fusions of the ctc pr...

Diller, W.; Bünger, P., 1965: On pharmacokinetics of rectal application, demonstrated on example of 4-sulfanilamido-5,6-dimethoxy-pyrimidine. 2. Experimental part. Arzneimittel-Forschung 15(12): 1445-1456

Smalldon, K.W., 1971: Two capillary microcell accessories for visible and ultra-violet spectroscopy. Medicine, Science, and the Law 11(4): 193-196

Lach, H.W.; Parsons, J.L., 2013: Impact of fear of falling in long term care: an integrative review. Long term care elders with fear of falling may restrict their activity resulting in declines in function and excess disability. To further explore this problem, a review of the literature was conducted. The search yielded 26 studies on the epidemi...

Cricchi, M.; Piccinelli, B., 1967: Thin-layer chromatography of phospholipids of the normal optic nerve in rabbits. Minerva Oftalmologica 9(6): 201-202

Sklar, L.A.; Dratz, E.A., 1982: Analysis of rod outer segment disk membrane phospholipid organization using parinaric acid fluorescent probes. Methods in Enzymology 81: 685-694

Al-Qurashi, A.; Maklad, K.M.; A.A.dulwahed, O., 2004: Pancreatitis due to ascaris lumbricoides, a case report. Ascaris is the 2nd commonest intestinal parasite world-wide. Although infection can pass asymptomatic, some can present with complications. The reported case is a Syrian female patient presented with acute on top of chronic pancreatitis due to A....

Lawrence, P.F.; Chandra, A., 2010: When should open surgery be the initial option for critical limb ischaemia?. The treatment paradigm for patients with critical limb ischaemia (CLI) has changed over the past decade with an increase in endovascular interventions. Accompanying this shift has been a fundamental question as to whether open surgery or endovascu...