+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

LARP4B is an AU-rich sequence associated factor that promotes mRNA accumulation and translation

, : LARP4B is an AU-rich sequence associated factor that promotes mRNA accumulation and translation. Rna 21(7): 1294-1305

mRNAs are key molecules in gene expression and subject to diverse regulatory events. Regulation is accomplished by distinct sets of trans-acting factors that interact with mRNAs and form defined mRNA-protein complexes (mRNPs). The resulting "mRNP code" determines the fate of any given mRNA and thus controlling gene expression at the post-transcriptional level. The La-related protein 4B (LARP4B) belongs to an evolutionarily conserved family of RNA-binding proteins characterized by the presence of a La-module implicated in direct RNA binding. Biochemical experiments have shown previously direct interactions of LARP4B with factors of the translation machinery. This finding along with the observation of an association with actively translating ribosomes suggested that LARP4B is a factor contributing to the mRNP code. To gain insight into the function of LARP4B in vivo we tested its mRNA association at the transcriptome level and its impact on the proteome. PAR-CLIP analyses allowed us to identify the in vivo RNA targets of LARP4B. We show that LARP4B binds to a distinct set of cellular mRNAs by contacting their 3' UTRs. Biocomputational analysis combined with in vitro binding assays identified the LARP4B-binding motif on mRNA targets. The reduction of cellular LARP4B levels leads to a marked destabilization of its mRNA targets and consequently their reduced translation. Our data identify LARP4B as a component of the mRNP code that influences the expression of its mRNA targets by affecting their stability.


PMID: 26001795

DOI: 10.1261/rna.051441.115

Other references

Lumsden, R.D.; Harrington, G.W., 1966: Incorporation of linoleic acid by the cestode Hymenolepis diminuta (Rudolphi, 1819). 14-day-old prepatent Hymenolepis diminuta were incubated in vitro in tris-maleate buffered salin-containing C14-labelled linoleic acid. These experie ments were designed to determine whether an externally supplied lipid can be absorbed by the worm...

Alonso-Fernández, J.I.; López-Messa, J.B.; Ortega-Sáez, M.; Calvo-Buey, J.; Gutiérrez-Alejandro, A.; Martínez-Martín, R., 2014: Results of an in-hospital cardiac arrest training and response program. Medicina Intensiva 38(9): 589-591

Deng, H.; Zuo, X.; Zhang, J.; Liu, X.; Liu, L.; Xu, Q.; Wu, Z.; Ji, A., 2016: Α‑lipoic acid protects against cerebral ischemia/reperfusion-induced injury in rats. It is well established that the brain is sensitive to ischemia/reperfusion (I/R)‑induced injury. α‑lipoic acid (LA), a free radical scavenger and antioxidant, has a neuroprotective effect against cerebral I/R‑induced injury, however, the un...

Terwilliger, K. ., 1991: Virginia's endangered species: proceedings of a symposium. Unknown

Béseler, M.R.; Grao, C.M.; Gil, A.; Martínez Lozano, M.D., 2013: Walking assessment with instrumented insoles in patients with lower limb spasticity after botulinum toxin infiltration. Botulinum toxin A (BTA) improves the kinematic parameters of gait in patients with spasticity of lower limbs, but there are no studies in which kinetic parameters are measured with instrumented insoles. We therefore used instrumented insoles to pe...

Henle, K., 1992: Zur Amphibienfauna Perus nebst Beschreibung eines neuen Eleutherodactylus (Leptodactylidae). A herpetological survey of Peru and the examination of further material in the collection of the Zoologisches Museum Alexander Koenig resulted in 94 species of amphibians. When known, ecological data and observations are provided for these species...

Sebela, M.; Frebort, I.; Pec, P., 1999: Quinoprotein amine oxidases - a new group of ubiquitous enzymes playing the key role in degradation of cellular polyamines. Amine oxidases (EC that contain copper/topa quinone cofactor are reviewed. In plants, these enzymes are involved in plant development and senescence, reduce the concentration of toxic amines produced during exposure to stress conditions,...

el-Ghamrawy, E.; Maeda, T., 1978: Crevicular temperature changes in relationship to plaque formation in partially endentulous subjects. Egyptian Dental Journal 24(4): 347-356

Hellerstein, D., 1992: Estimating consumer surplus in the censored linear model. Land economics 68(1): 83-92

Ahmed, A.M.; Kandil, M.H.; E.S.aer, H.M.; Metawi, H.R., 2001: Performance of desert black goat under extensive production systems in North Sinai in Egypt. A production system approach was used to study the performance of desert black goat under semiarid conditions in North Sinai. A random sample of 19 goat breeders was taken. Least squares analysis of variance was performed using fixed effect linear...