geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Laminin γ2 knockout mice rescued with the human protein exhibit enamel maturation defects


, : Laminin γ2 knockout mice rescued with the human protein exhibit enamel maturation defects. Matrix Biology 52-54: 207-218

The epithelial ameloblasts are separated from the maturing enamel by an atypical basement membrane (BM) that is enriched in laminin 332 (LM-332). This heterotrimeric protein (α3, ß3 and γ2 chains) provides structural integrity to BMs and influences various epithelial cell processes including cell adhesion and differentiation. Mouse models that lack expression of individual LM-332 chains die shortly after birth. The lethal phenotype of laminin γ2 knockout mice can be rescued by human laminin γ2 (LAMC2) expressed using a doxycycline-inducible (Tet-on) cytokeratin 14 promoter-rtTA. These otherwise normal-looking rescued mice exhibit white spot lesions on incisors. We therefore investigated the effect of rescue with human LAMC2 on enamel maturation and structuring of the atypical BM. The maturation stage enamel organ in transgenic mice was severely altered as compared to wild type controls, a structured BM was no longer discernible, dystrophic matrix appeared in the maturing enamel layer, and there was residual enamel matrix late into the maturation stage. Microtomographic scans revealed excessive wear of occlusal surfaces on molars, chipping of enamel on incisor tips, and hypomineralization of the enamel layer. No structural alterations were observed at other epithelial sites, such as skin, palate and tongue. These results indicate that while this humanized mouse model is capable of rescue in various epithelial tissues, it is unable to sustain structuring of a proper BM at the interface between ameloblasts and maturing enamel. This failure may be related to the atypical composition of the BM in the maturation stage and reaffirms that the atypical BM is essential for enamel maturation.

US$19.90

PMID: 26956061

DOI: 10.1016/j.matbio.2016.03.002


Other references

Lee, S.R., 1986: Psychiatric management during pregnancy. Journal of the Medical Association of Georgia 75(10): 617-621

Liew, S.Fatt.; Cao, H., 2015: Modification of light transmission channels by inhomogeneous absorption in random media. Optical absorption is omnipresent and often distributed non-uniformly in space. We present a numerical study on the effects of inhomogeneous absorption on transmission eigenchannels of light in highly scattering media. In the weak absorption regim...

Anonymous, 2012: Gonadal and extragonadal germ cell tumours in the United States, 1973–27. Germ cell tumours (GCTs) most often arise in the gonads, but some develop extragonadally. The aim of this study was to examine gender- and race-specific trends in incidence and survival of gonadal (GGCTs) and extragonadal GCTs (EGCTs) in the US fr...

L.Societe Francophone D.Primatologie, 2001: 12th Annual Meeting of La Societe Francophone de Primatologie. Folia Primatologica 72(3): 115-127, September

Prieto-Rincón, D.; Piñerúa-Shuhaibar, L.; Bonilla, E.; Prasad, A.; Arrieta, A., 1991: Paranoid schizophrenia: free amino acids in the cerebrospinal fluid. The authors reported an increased in the cerebrospinal fluid concentration of glutamine in seven paranoid schizophrenic patients. They found no changes in the levels of the remaining 20 free amino acids assayed.

Uckele J.; Mcdonald J.W.; Johnston M.; Silverstein F., 1989: Glycine antagonists attenuate n methyl d aspartate nmda mediated brain injury. Pediatric Research 25(4 PART 2): 362A

Bao, X.; Wang, W-jiu.; Dai, N., 2013: Study of the correlation between 51 immune infertility patients of the Han nationality in Anhui and HLA-DQA1 gene and treatment of Mianbu III. To explore the correlation between anti-sperm antibody-positive immune infertility patients and human leucocyte antigen (HLA) DQA1 gene, and to study the correlation between the treatment of Chinese medicine and pharmacy and HLA-DQA1 genotype. The...

Greenberg, D.S., 1995: Vows of cuts and glimmers of hope. Lancet 345(8944): 245-245

Shcherbin S.S.; Osetrov O.A., 1963: Pervichnye oreoly rasseyaniya redkikh elementov pegmatitov kak kriterii poiskov skrytogo orudeneniya. Lithium and other rare elements expelled from pegmatites during processes of albitization, greisenization, or other alteration processes form primary aureoles of varying widths and intensities, which are important guides to occurrences of conceale...

Zajjari, Y.; Aatif, T.; Bahadi, A.; Hassani, K.; E.K.bbaj, D.; Benyahia, M., 2015: Kidney biopsy in the Military Hospital of Morocco: Complications and histopathological findings. Epidemiological studies on renal biopsies are necessary to establish the pattern and trends of renal diseases in a particular geographic area. In this retrospective study, we reviewed the medical records, histopathology findings and complications...