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Lysophosphatidylcholines activate PPARδ and protect human skeletal muscle cells from lipotoxicity


, : Lysophosphatidylcholines activate PPARδ and protect human skeletal muscle cells from lipotoxicity. Biochimica et Biophysica Acta 1861(12 Pt A): 1980-1992

NlmCategory="UNASSIGNED">Metabolomics studies of human plasma demonstrate a correlation of lower plasma lysophosphatidylcholines (LPC) concentrations with insulin resistance, obesity, and inflammation. This relationship is not unraveled on a molecular level. Here we investigated the effects of the abundant LPC(16:0) and LPC(18:1) on human skeletal muscle cells differentiated to myotubes. Transcriptome analysis of human myotubes treated with 10μM LPC for 24h revealed enrichment of up-regulated peroxisome proliferator-activated receptor (PPAR) target transcripts, including ANGPTL4, PDK4, PLIN2, and CPT1A. The increase in both PDK4 and ANGPTL4 RNA expression was abolished in the presence of either PPARδ antagonist GSK0660 or GSK3787. The induction of PDK4 by LPCs was blocked with siRNA against PPARD. The activation of PPARδ transcriptional activity by LPC was shown as PPARδ-dependent luciferase reporter gene expression and enhanced DNA binding of the PPARδ/RXR dimer. On a functional level, further results show that the LPC-mediated activation of PPARδ can reduce fatty acid-induced inflammation and ER stress in human skeletal muscle cells. The protective effect of LPC was prevented in the presence of the PPARδ antagonist GSK0660. Taking together, LPCs can activate PPARδ, which is consistent with the association of high plasma LPC levels and PPARδ-dependent anti-diabetic and anti-inflammatory effects.

US$19.90

PMID: 27697477

DOI: 10.1016/j.bbalip.2016.09.020


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Rosado Neto, G.H.; Lima, I.M. de M., 2002: Odontopus brevirostris (Hustache, 1936) (Coleoptera, Curculionidae) associated with new host plants belonging to Annona (Annonaceae). Odontopus brevirostris (Hustache, 1936) feeding on Annona squamosa L., A. cherimola Mill., A. glabra L., and A. muricata L. was observed. The last three host plants are recorded for the first time. The endophitic oviposition occurs in the veins of...

Nakamura-Stoecklin, U., 1977: Japan. Zeitschrift für Krankenpflege. Revue Suisse des Infirmieres 70(3): 60-68

Anonymous, 1993: France: local sports economy. The functioning of sport at the local level in France is examined. Sports clubs received almost 2 million F in 1989 in direct subsidies from communes, excluding staff and equipment provision. Almost all sports associations are subsidized and they...

Walker, R.; Rahim, A.; Parke, D.V., 1973: The effects of antioxidants on enzyme induction in developing and ageing rats. Proceedings of the Royal Society of Medicine 66(8): 780-780

Hernández, Bén.; Pflüger, F.; Adenier, A.; Kruglik, S.G.; Ghomi, M., 2011: Vibrational analysis of amino acids and short peptides in hydrated media. VIII. Amino acids with aromatic side chains: L-phenylalanine, L-tyrosine, and L-tryptophan. Four out of the 20 natural α-amino acids (α-AAs) contain aromatic rings in their side chains. In a recent paper (J. Phys. Chem. B 2010, 114, 9072-9083), we have analyzed the structural and vibrational features of l-histidine, one of the potent e...

Klewin K.W.; Berg J.H., 1989: Evolution of the Midcontinent Rift; a perspective from the stratigraphy and chemistry of the Mamainse Point Volcanics, Ontario. Eos, Transactions, American Geophysical Union 70(15): 273-274

Bat Miriam Katznelson M.; Menczer J.; Avivi L., 1988: Dermatoglyphic studies in cancer of the cervix and of the endometrium. American Journal of Human Genetics 43(3 SUPPL): A37

Carmo, C.Almeida.; Batistel, F.; de Souza, J.; Martinez, J.Cesar.; Correa, P.; Pedroso, A.Mendonça.; Santos, Fávio.Augusto.Portela., 2015: Starch levels on performance, milk composition and energy balance of lactating dairy cows. The objective of this experiment was to evaluate the effects of starch levels in diets with the replacement of citrus pulp for corn on milk yield, milk composition, and energy balance of lactating dairy cows. Twenty-eight multiparous Holstein cows...

Prikhozhan, V.M., 1987: Classification of diabetic neuropathy. Problemy Endokrinologii 33(3): 79-85

Aukrust, P.; Müller, F.; Ueland, T.; Svardal, A.M.; Berge, R.K.; Frøland, S.S., 2000: Decreased vitamin A levels in common variable immunodeficiency: vitamin A supplementation in vivo enhances immunoglobulin production and downregulates inflammatory responses. Background: Vitamin A has a broad range of immunological effects, and vitamin A deficiency is associated with recurrent infections. Common variable immunodeficiency (CVI) is a group of B-cell deficiency syndromes with impaired antibody production...