geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Male meiotic cytokinesis requires ceramide synthase 3-dependent sphingolipids with unique membrane anchors


, : Male meiotic cytokinesis requires ceramide synthase 3-dependent sphingolipids with unique membrane anchors. Human Molecular Genetics 24(17): 4792-4808

Somatic cell cytokinesis was shown to involve the insertion of sphingolipids (SLs) to midbodies prior to abscission. Spermatogenic midbodies transform into stable intercellular bridges (ICBs) connecting clonal daughter cells in a syncytium. This process requires specialized SL structures. (1) Using high resolution-mass spectrometric imaging, we show in situ a biphasic pattern of SL synthesis with testis-specific anchors. This pattern correlates with and depends on ceramide synthase 3 (CerS3) localization in both, pachytene spermatocytes until completion of meiosis and elongating spermatids. (2) Blocking the pathways to germ cell-specific ceramides (CerS3-KO) and further to glycosphingolipids (glucosylceramide synthase-KO) in mice highlights the need for special SLs for spermatid ICB stability. In contrast to somatic mitosis these SLs require ultra-long polyunsaturated anchors with unique physico-chemical properties, which can only be provided by CerS3. Loss of these anchors causes enhanced apoptosis during meiosis, formation of multinuclear giant cells and spermatogenic arrest. Hence, testis-specific SLs, which we also link to CerS3 in human testis, are quintessential for male fertility.

US$19.90

PMID: 26045466

DOI: 10.1093/hmg/ddv204


Other references

Simonsson, P.; Mårtensson, A.; Rustad, Pål., 2004: New common reference intervals for clinical chemistry in the Nordic countries. A better basis for clinical assessment and cooperation. A project engaging the five Nordic countries has presented common reference intervals for the most frequently used biochemical and haematological analytes. The results are based on samples from up to 3000 healthy adult reference persons and are st...

Mcnichols, R.J.; Gowda, A., 2003: Method and system for controlling heat delivery to a target. A method and system for using real time closed loop feedback to control the delivery of heat energy is disclosed herein. An energy delivery system may be used to deliver heat energy to a target to effect a change in the target. For example, medica...

Stevens, C., 2001: Value chains and trade policy: the case of agriculture. Value-chain and traditional trade policy analysis are complementary, each throws light on areas overlooked by the other. Value-chain analysis helps to identify who gains from market imperfections and how the distribution of gains can be altered. T...

Björnhagen, V.; Erhardt, K.; Lagerlöf, B.; Auer, G., 1991: Comparison of nuclear DNA content in primary and metastatic malignant melanoma. The DNA patterns obtained from 23 primary malignant melanomas and 35 corresponding metastases were compared and found to differ in many cases. In eight cases the primary tumors and their metastases had a ploidy type I ("euploid") DNA pat...

Laverdant, C.; Molinié, C.; Cristau, P.; Essioux, H.; Daly, J.P., 1983: Value of laparoscopy in the early diagnosis of digestive schistosomiasis. Schistosoma mansoni, humans (young adults) with invasive phase of digestive tract infections, laparoscopy a valuable tool in early diagnosis, review of 181 cases in Tchad

Vaporciyan, A.A.; Merriman, K.W.; Ece, F.; Roth, J.A.; Smythe, W.Roy.; Swisher, S.G.; Walsh, G.L.; Nesbitt, J.C.; Putnam, J.B., 2002: Incidence of major pulmonary morbidity after pneumonectomy: association with timing of smoking cessation. The prevention of major pulmonary events (MPEs) after pneumonectomy may minimize postoperative mortality rates. The purpose of this study was to identify preoperative and perioperative factors associated with the development of MPEs after pneumone...

Meek A.C.; Lane I.F.; Chidlow A.; Harper R.A.; Mccollum C.N., 1987: Indium 111 platelet deposition and its relation to restenosis and occlusion in ptfe grafts. Nuklearmedizin 26(4): 110

Jenkins, J.M.; Covington, H.M., 1950: Growing lettuce in North Carolina. Soils such as Portsmouth, Bladen, and Appling are suitable for lettuce production in N. C. On light sandy soils 1500 lbs. per acre of a 6-8-6 fertilizer is recommended but on heavier soils a 4-10-6 usually gives better results. Great Lakes is the...

Gurd, F.N.; Hampson, L.G.; Gibson, W.J.; Innes, B.J.; Mulder, D.S., 1966: The value of a clinical shock study protocol in the management of refractory shock. Journal of Trauma 6(2): 157-175

Brownlow, H.A.; Sutherland, P.D., 2002: Joint disagreement. Anaesthesia 57(11): 1142-1142