geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Molecular authentication of Gynostemma pentaphyllum through development and application of random amplification polymorphic DNA sequence-characterized amplified region marker


, : Molecular authentication of Gynostemma pentaphyllum through development and application of random amplification polymorphic DNA sequence-characterized amplified region marker. Genetics and Molecular Research 14(4): 16204-16214

Due to the morphological similarities of aerial parts, it is difficult to distinguish Gynostemma pentaphyllum from Cayratia japonica, which is usually an adulterant of the former. To develop a reliable method for the identification and authentication of G. pentaphyllum, a combination of random amplification polymorphic DNA (RAPD) technique with sequence-characterized amplified region (SCAR) markers was studied. Twenty-five samples of G. pentaphyllum and two samples of C. japonica were collected from different regions in Guangxi or bought from different provinces in China. Through the RAPD analysis, significant genetic polymorphism was observed among the intraspecies samples of G. pentaphyllum. Furthermore, a specific marker, J-750, was obtained for authentication. Therefore, the SCAR marker for G. pentaphyllum (359 bp) was developed from the RAPD amplicon. With PCR amplification using the SCAR primers, a specific band of 359 bp was distinctly visible for all tested samples of G. pentaphyllum, but was absent in the samples of C. japonica. Furthermore, the results revealed that the SCAR marker was useful for the identification and authentication of G. pentaphyllum irrespective of whether samples were fresh, dry, or of commercial origin. The SCAR marker obtained in this study successfully authenticated G. pentaphyllum through an integrated PCR system containing SCAR and control primer combinations of two pairs. In addition, it was also used for simultaneous discrimination of G. pentaphyllum from C. japonica.

US$19.90

PMID: 26662413

DOI: 10.4238/2015.December.8.10


Other references

Cremer, T.; Peterson, S.P.; Cremer, C.; Berns, M.W., 1981: Laser microirradiation of Chinese hamster cells at wavelength 365 nm: effects of psoralen and caffeine. Radiation Research 85(3): 529-543

Lowe, O., 1986: Pretreatment dental assessment and management of patients undergoing head and neck irradiation. Clinical Preventive Dentistry 8(3): 24-30

Xu, Q.; Nakayama, M.; Suzuki, Y.; Sakai, K.; Nakamura, T.; Sakai, Y.; Matsumoto, K., 2012: Suppression of acute hepatic injury by a synthetic prostacyclin agonist through hepatocyte growth factor expression. Previous studies have demonstrated that mice disrupted with the cyclooxygenase-2 gene showed much more severe liver damage compared with wild-type mice after liver injury, and prostaglandins (PGs) such as PGE<SUB>1/2</SUB> and PGI<S...

Hathaway, I., 1990: Record of important family papers--personal and financial records. Extension bulletin E Cooperative Extension Service Michigan State University: 90 (451)

Botelho, J.R.; Williams, P., 1980: Ectoparasites of wild rodents in Caratinga County, Minas Gerais, Brazil. II. Acarian fauna. Memorias do Instituto Oswaldo Cruz 75(3-4): 47-51

Leomil Neto, M., 2006: Clinical evaluation of dogs with dilated cardiomyopathy (DCM) treated by carvedilol. Dilated cardiomyopathy (DCM) is a frequent disease in veterinary clinics which occurs specially in dogs, mostly males, young or young-adults, of big and giant breeds, mainly Dobermans and Boxers. The disease is characterized by a reduction in cont...

Panchishina, M.V.; Vikhrova, F.K., 1975: Use of colibacterin in chronic gastritis. Vrachebnoe Delo: 63-65

Chaudhury H.M., 1971: Seismological Notes. Earthquakes, India, July-September, 1970.

Kumar A.; Rogers T.; Maizel A.; Sharma S., 1990: Differential growth control of ebv positive and ebv negative malignant b cells by tgf beta in response to r bcgf 12kd. Lymphokine Research 9(4): 601

Salomon, J.C.; Gueguen, S.; Lazar, P., 1970: Immunodepressive effect of methylcholanthrene in mice. Secondary immune response and survival. Pathologie-Biologie 18(1): 65-68