geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

New mass-spectrometry-compatible degradable surfactant for tissue proteomics


, : New mass-spectrometry-compatible degradable surfactant for tissue proteomics. Journal of Proteome Research 14(3): 1587-1599

Tissue proteomics is increasingly recognized for its role in biomarker discovery and disease mechanism investigation. However, protein solubility remains a significant challenge in mass spectrometry (MS)-based tissue proteomics. Conventional surfactants such as sodium dodecyl sulfate (SDS), the preferred surfactant for protein solubilization, are not compatible with MS. Herein, we have screened a library of surfactant-like compounds and discovered an MS-compatible degradable surfactant (MaSDeS) for tissue proteomics that solubilizes all categories of proteins with performance comparable to SDS. The use of MaSDeS in the tissue extraction significantly improves the total number of protein identifications from commonly used tissues, including tissue from the heart, liver, and lung. Notably, MaSDeS significantly enriches membrane proteins, which are often under-represented in proteomics studies. The acid degradable nature of MaSDeS makes it amenable for high-throughput MS-based proteomics. In addition, the thermostability of MaSDeS allows for its use in experiments requiring high temperature to facilitate protein extraction and solubilization. Furthermore, we have shown that MaSDeS outperforms the other MS-compatible surfactants in terms of overall protein solubility and the total number of identified proteins in tissue proteomics. Thus, the use of MaSDeS will greatly advance tissue proteomics and realize its potential in basic biomedical and clinical research. MaSDeS could be utilized in a variety of proteomics studies as well as general biochemical and biological experiments that employ surfactants for protein solubilization.

US$19.90

PMID: 25589168

DOI: 10.1021/pr5012679


Other references

Nagington, J.; Sutehall, G.M.; Whipp, P., 1983: Tonometer disinfection and viruses. Tonometer disinfectants in current use are frequently inactive against viruses and enable transfer of infection between patients. We selected sodium hypochlorite as a readily available alternative which is active against viruses and bacteria. At 5...

Burnett, S., 1994: Mental health. A new lease on life. Nursing Times 90(31): 57-59

Lilley K.S.; Hornby D.P.; Engel P.C., 1988: Purification chemical modification and analysis of ph dependence of the nadp dependent glutamate dehydrogenase of escherichia coli. Biochemical Society Transactions 16(5): 876-877

Chowdhury, S.; Sengupta, N., 2002: Rational choice of oral antihyperglycaemic agents. Until 1995 sulphonylureas and metformin formed the mainstay of oral pharmacotherapy of type 2 diabetes mellitus. Since then many new insulin secretagogues and new classes of oral antihyperglycaemic agents have been launched. This has improved the...

Szerlip H.M.; Palevsky P.; Cox M.; Blazer Yost B., 1991: Relationship of the aldosterone induced protein gp70 to the conductive sodium channel. Although one of the primary effects of aldosterone is to increase apical membrane Na+ conductance, as yet none of the proteins induced by the hormone in renal epithelia have been shown to be related to the conductive Na+ channel. Because the toad...

Tkachenko, A.A.; Negovskii, N.A., 1975: New initial material for breeding monogerm sugar beet. When Belotserkovskaya odnosemyannaya [Belaya Tserkov' Monogerm] was crossed with a number of varieties, the highest root and sugar yields were obtained from hybrids whose female parent was a multigerm variety. These hybrids were back crossed...

Calderwood, D., J.; Johnston, D., N.; Munschauer, R.; Rafferty, P., 2002: Pyrrolo(2,3-d)pyrimidines containing diverse N-7 substituents as potent inhibitors of lck. A series of pyrrolo(2,3-d)pyrimidines was synthesized and evaluated as inhibitors of Lck. Lck accommodates a diverse set of substituents at N-7. Altering the substituent at N-7 provided compound 13, an orally available lck inhibitor which inhibite...

Domenech, J.; Heraud, N.; Dayan, A.; Estienne, M.H.; Linassier, C.; Colombat, P.; Binet, C., 1995: Decreased proliferative capacity of marrow CD34+ cells after autologous bone marrow transplantation. Experimental Hematology (Charlottesville) 23(8): 803

Turner, B.C.; Lehman, T.A.; Haffty, B.; Bishop, L.; Carbone, C.; Gumbs, A.; Lozano, G.; Strong, L.; Modali, R., 1999: High frequency and functionally activity of a specific germline p53 intron mutation in familial breast cancer. Proceedings of the American Association for Cancer Research Annual Meeting 40: 194-195, March

Mengozzi, B., 2004: EU new entries: Czech Republic, farmers face crisis. In the Czech Republic, agricultural production accounts for a rapidly diminishing share of the economy and farm workers are moving to other sectors. The industry is changing, but farming remains technologically backward.