+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Oxysterol-binding protein-related protein 4L promotes cell proliferation by sustaining intracellular Ca2+ homeostasis in cervical carcinoma cell lines

, : Oxysterol-binding protein-related protein 4L promotes cell proliferation by sustaining intracellular Ca2+ homeostasis in cervical carcinoma cell lines. Oncotarget (): -

NlmCategory="UNASSIGNED">Oxsterol binding protein-related protein 4 (ORP4) is essential for cell proliferation, but the underlying mechanism is unclear. ORP4 is expressed as three variants, ORP4L, ORP4M and ORP4S. Here, we reported that silencing of ORP4L with specific small interfering RNA (siRNA) inhibited the proliferation of human cervical cancer cell lines C33A, HeLa and CaSki, the reverse effect being observed in ORP4L overexpressing cells. For molecular insight, we found that ORP4L maintained intracellular Ca2+ homeostasis. Through this mechanism, ORP4L activated nuclear factor of activated T cells (NFAT) activity and thus promoted expression of a gene cluster which supported cell proliferation. Of note, ORP4L sustained inositol-1,4,5-trisphosphate receptor 1 (IP3R1) expression at both mRNA and protein levels via Ca2+-dependent NFAT3 activation, which offered a mechanic explanation for the role of ORP4L intracellular Ca2+ homeostasis. Furthermore, ORP4L knockdown markedly inhibited tumor growth in a C33A cell xenograft mouse model. To conclude, our results reveal that ORP4L promotes cell proliferation through maintaining intracellular Ca2+ homeostasis.


PMID: 27588468

DOI: 10.18632/oncotarget.11671

Other references

Evans S.J.; Khan S.S.; Garfinkel A.; Kass R.M.; Albano A.; Diamond G.A., 1989: Is ventricular fibrillation random or chaotic. Circulation 80(4 SUPPL 2): II134

von Waltershausen Wolfgang Sartorius, 1850: Iceland, its climate and its geysers. Annual of Scientific Discovery Year-book of Facts in Science and Art 1: 231-232

Foos, R.Y.; Hull, S.N.; Straatsma, B.R., 1969: Early diagnosis of ciliary body melanomas. Archives of Ophthalmology 81(3): 336-344

Ullmann W., 1984: Boundary condition for the inhomogeneity parameter in the Earth; implications for heat action within the upper mantle. The inhomogeneity parameter Delta is a measure of departure from the Williamson-Adams requirement for a homogeneous Earth. It is shown that Delta + 1 must be positive, without eceeding the ratio C (sub P) /C (sub V) , where C (sub P) and C (sub V)...

Muñoz Herrera, A.; de la Fuente Jambrina, C.; Pérez Plasencia, D.; Santa-Cruz Ruiz, S.; Blanco Pérez, P.; Benito Gómez, J., 2003: Epidermoid cyst of the parapharyngeal space. Epidermal cysts of parapharyngeal location are very unusual tumors, with a benign character, though when they reach a great size, adjacent zones and vascular and nervous structures of great importance can be affected. We are going to describe the...

Anonymous, 2006: Aleurodicus dispersus.. A new distribution map is provided for Aleurodicus dispersus Russell. Hemiptera: Aleyrodidae. Hosts: Polyphagous, including avocado (Persea americana), cassava (Manihot esculenta), coconut (Cocos nucifera), guava (Psidium guajava), soyabean (Glyci...

Dubini, F.; Bolloli, A., 1969: Antimitotic activity of lithium chloride on human leukocytes in culture. Archivio Italiano di Patologia E Clinica Dei Tumori 12(1): 79-82

Hedley Whyte J., 1977: Distribution of 9 10 tritiated di palmitoyl lecithin in rat lung. Physiologist: 32

Oxberry, J.M.; Gregson, N.A., 1974: The agglutination of myelin suspensions by specific antisera. Brain Research 78(2): 303-313

Shorten, A.; Shorten, B., 2000: Women's choice? The impact of private health insurance on episiotomy rates in Australian hospitals. To assess the extent to which variations in episiotomy rates in Australian hospitals are justified by clinical variables and to further explore the relationships between episiotomy, insurance status, perineal trauma and outcomes for babies. A retr...