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Peripheral leukocyte expression of the potential biomarker proteins Bdnf, Sirt1, and Psen1 is not regulated by promoter methylation in Alzheimer's disease patients

, : Peripheral leukocyte expression of the potential biomarker proteins Bdnf, Sirt1, and Psen1 is not regulated by promoter methylation in Alzheimer's disease patients. Neuroscience Letters 605: 44-48

The identification of Alzheimer's disease (AD) biomarkers is crucial to support drug discovery. Within putative biomarkers, peripheral Bdnf levels correlate with cognitive decline and AD, although conflicting findings are reported. Sirtuin 1 (Sirt1) serum levels are lower in AD patients and Presenilin 1 (Psen1) is expressed by blood cells. DNA methylation is altered in AD patients, suggesting that epigenetic mechanisms play a role in AD pathophysiology. The objective of this study was to investigate promoter methylation levels of potential biomarkers in AD cases and controls. Peripheral blood DNA methylation levels were analysed by methylation-specific primer real-time PCR. Bdnf promoter methylation levels did not differ between AD patients and controls. Similarly, Sirt1 promoter revealed minimal levels of methylation which did not display significant differences between groups. No significant difference was revealed between AD patients and controls also in Psen1 methylation, showing a large variability of values among subjects. Although peripheral Bdnf expression is associated with differential promoter methylation in psychiatric and neurological disorders, our results suggest that different mechanisms take place in AD. The finding that the control of Sirt1 protein levels in blood is not exerted through the repression of mRNA expression by promoter hypermethylation is in agreement with previous data. In contrast, other studies reported that Psen1 methylation may be increased or decreased in AD patients, suggesting that additional studies are required. In conclusion, this study shows that peripheral levels of the potential AD biomarker proteins Bdnf, Sirt1, and Psen1 are not regulated by different promoter methylation.


PMID: 26275347

DOI: 10.1016/j.neulet.2015.08.012

Other references

Chowdhury P.; Chang L.W.; Bone R.C.; Rayford P.L., 1981: Promotion of cadmium induced pulmonary lesions by galactosamine. Physiologist 24(4): 106

Bischoff, K., 1979: Unterrichtsgang durch das Vivarium. - 7. Wildtiere und Haustiere. Naturwissenschaftlicher Verein Darmstadt Bericht N F: 19781: 2-6

Sklar L.A.; Miljanich G.P.; Bursten S.L.; Dratz E.A., 1979: Thermal lateral phase separations in bovine retinal rod outer segment membranes and phospho lipids as evidenced by parinaric acid fluorescence polarization and energy transfer. The temperature dependence of the fluorescence polarization of cis-parinaric acid (9,11,13,15-cis,trans,trans,cis-octadecatetraenoic acid), trans-parinaric acid (9,11,13,15-all-trans-octadecatetraenoic acid) and their methyl ester derivatives was...

Court-Payen, 1970: Use of the Guillerey technic in the treatment of obesity. Rivista Sperimentale di Freniatria E Medicina Legale Delle Alienazioni Mentali 94(5): 1223-1230

Noble, LR.; Jones, CS., 1996: A molecular and ecological investigation of the large arionid slugs of north-west Europe the potential for new pests. Systematics Association Special Volume Series, 53: 93-131

Lortet.; Despeignes., 1892: Vens de terre et Tuberculose. Compte Rendu, cxv 66

Feldman, S., 1981: Groundnut cultivation: Israel obtains high yields. The mean annual seed yield in the Besor area of the Negev is >5.5 t/ha and of hay >3 t. The major cv. used is the Shulamit. The agronomy of the crop is described. In some fields in the SE Negev, Ca induced chlorosis was alleviated using 7-10...

Terranova Remo, 1964: Le brecce dei Monti Aiona e Penna nell'Appennino Ligure-Emiliano (nota preliminare). Thirteen different breccias found in the sedimentary and ophiolitic rocks of Monte Aiona and Monte Penna (Italy) are classified as follows: two breccias belong to a stratified sedimentary facies, four breccias belong to ophiolitic facies, and seve...

Bechtold, R.E.; Dyer, R.B.; Zagoria, R.J.; Chen, M.Y., 1996: The perirenal space: relationship of pathologic processes to normal retroperitoneal anatomy. The perirenal space may be involved by disease processes that arise within or outside the perirenal space. Key anatomic details that dictate the features of perirenal processes include the renal capsule, the perirenal septa, the renal fascia, and...

Wickware, A.B., 1922: An unusual form of seabies in fowls. Journal of Parasitology Urbana, 8: 90-91