+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Pli1(PIAS1) SUMO ligase protected by the nuclear pore-associated SUMO protease Ulp1SENP1/2

, : Pli1(PIAS1) SUMO ligase protected by the nuclear pore-associated SUMO protease Ulp1SENP1/2. Journal of Biological Chemistry 290(37): 22678-22685

Covalent modification of the proteome by SUMO is critical for genetic stability and cell growth. Equally crucial to these processes is the removal of SUMO from its targets by the Ulp1 (HuSENP1/2) family of SUMO proteases. Ulp1 activity is normally spatially restricted, because it is localized to the nuclear periphery via interactions with the nuclear pore. Delocalization of Ulp1 causes DNA damage and cell cycle defects, phenotypes thought to be caused by inappropriate desumoylation of nucleoplasmic targets that are normally spatially protected from Ulp1. Here, we define a novel consequence of Ulp1 deregulation, with a major impact on SUMO pathway function. In fission yeast lacking Nup132 (Sc/HuNUP133), Ulp1 is delocalized and can no longer antagonize sumoylation of the PIAS family SUMO E3 ligase, Pli1. Consequently, SUMO chain-modified Pli1 is targeted for proteasomal degradation by the concerted action of a SUMO-targeted ubiquitin ligase (STUbL) and Cdc48-Ufd1-Npl4. Pli1 degradation causes the profound SUMO pathway defects and associated centromere dysfunction in cells lacking Nup132. Thus, perhaps counterintuitively, Ulp1-mediated desumoylation can promote SUMO modification by stabilizing a SUMO E3 ligase.


PMID: 26221037

DOI: 10.1074/jbc.M115.673038

Other references

Gaffan, E.A.; Gaffan, D.; Harrison, S., 1988: Disconnection of the amygdala from visual association cortex impairs visual reward-association learning in monkeys. Cynomolgus monkeys (Macaca fascicularis) were trained in a task that assessed their ability to associate visual stimuli with food reward. Acquisition of stimulus-reward associations was measured under 2 conditions, a 2-stimuli acquisition conditio...

Jagadeeswaran, Pudur., 2006: Annual fish as a genetic model for aging. Unknown

Boissonnas, A.; Combadiere, C.; Lavergne, E.; Maho, M.; Blanc, C.; Debré, P.; Combadiere, B., 2004: Antigen distribution drives programmed antitumor CD8 cell migration and determines its efficiency. Understanding both the role of tumor Ag in CD8 cell differentiation and the reasons that CD8 cells may work inefficiently is crucial for therapeutic approaches in cancer. We studied OT-1 CD8 cell responses in vivo in a differential Ag-distribution...

Jackson, S.J.; Bluck, L.J.C.; Coward, W.Andrew., 2004: Use of isotopically labelled octanoic acid to assess the effect of meal size on gastric emptying. It has been proposed that the (13)C-octanoic acid breath test (OBT) provides a safe, non-radioactive means of measuring gastric emptying. However, deuterated octanoic acid provides a better marker when compared with scintigraphy, as the kinetics a...

Colwell J.A.; Halushka P.V.; Sarji K.E.; Lopes Virella M.F.; Sagel J., 1979: Vascular disease in diabetes patho physiological mechanisms and therapy. Vascular disease is the major cause of morbidity and mortality in diabetes mellitus. Diabetics are twice as prone to heart attacks, 5 times more prone to gangrene, 17 times more prone to kidney failure and 25 times more prone to blindness than non...

Podgorny, E., 1975: Increased participation of farmers circles in management of agricultural production. i. Nowe rolnictwo: 31, 24 (13 14) 14-16

Jian-Rong, D.; Yun-Tian, X.; Hong-Jun, L.; Wei, W.; Yong-Hui, T.; Guo-Li, Q.; You-Zi, L.; Jian-Ying, W.; You-Sheng, L., 2012: Studies on spray of niclosamide ethanolamine salt. I. development and effect assessment of spray of niclosamide ethanolamine salt. To develop a spray of niclosamide ethanolamine salt for prevention of bovine from Schistosoma japonicum infection, and explore its characteristics and effect. The solubilizers, penetrating agents, emulsifiers were screened, and the spray of niclos...

Rodríguez-Arámbula, A.; Arenas-Velázquez, E.; Castanedo-Cázares, J.Pablo.; Hernández-Blanco, D.; Oros-Ovalle, Cémoc.; Torres-Álvarez, B., 2016: Allopurinol hypersensitivity syndrome. A report of two cases. Los pacientes bajo tratamiento con alopurinol pueden presentar reacciones adversas potencialmente mortales, particularmente al inicio del tratamiento. Las reacciones cutáneas adversas por alopurinol tienen una prevalencia aproximada del 2 %. Pres...

Jiao K.; Robbat A.Jr, 1992: Determination of priority pollutant base neutral compounds in soil by a mobile thermal desorption gas chromatography mass spectrometry. Abstracts of Papers American Chemical Society 204(1-2): ANYL 14

Kitamura D.; Roes J.; Kuehn R.; Rajewsky K., 1991: A b cell deficient mouse by targeted disruption of the membrane exon of the immunoglobulin mu chain gene. Nature (London) 350(6317): 423-426