+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Protein Kinase A Signaling Is Inhibitory for Reprogramming into Pluripotent Stem Cells

, : Protein Kinase A Signaling Is Inhibitory for Reprogramming into Pluripotent Stem Cells. Stem Cells and Development 25(5): 378-385

Somatic cells may be reprogrammed into pluripotent cells by the ectopic expression of defined transcription factors. However, some of the hurdles that affect the generation of induced pluripotent stem cells include extremely low efficiency and slow reprogramming. In the present study, we examined the effects of small molecules on cellular reprogramming and found that 8-Bromoadenosine 3',5'-cyclic monophosphate (8-Br-cAMP), an analog of cyclic adenosine monophosphate (cAMP), improves the reprogramming efficiency of reprogrammable mouse fibroblasts induced with dox in serum replacement (SR) medium. Interestingly, treatment with 8-Br-cAMP in mouse embryonic stem cell culture conditions does not affect reprogramming into the pluripotent state; however, reprogramming efficiency is significantly enhanced by inhibition of protein kinase A (PKA) in SR medium. Therefore, our results suggest that PKA signaling is unnecessary and may in fact act as a barrier to reprogramming into pluripotent stem cells.


PMID: 26728702

DOI: 10.1089/scd.2015.0333

Other references

Giammanco, G.Maurizio.; De Grazia, S.; Bonura, F.; Cappa, V.; Muli, S.Li.; Pepe, A.; Medici, M.Cristina.; Tummolo, F.; Calderaro, A.; Di Bernardo, F.; Dones, P.; Morea, A.; Loconsole, D.; Catella, C.; Terio, V.; Bànyai, Kàn.; Chironna, M.; Martella, V., 2017: Norovirus GII.17 as Major Epidemic Strain in Italy, Winter 2015-16. In winter 2015-16, norovirus GII.17 Kawasaki 2014 emerged as a cause of sporadic gastroenteritis in children in Italy. Median patient age was higher for those with GII.17 than GII.4 infection (55 vs. 24 months), suggesting limited cross-protection...

Chen XiaoXiang; Ding XiaoYing; Chen Si, 2004: The study of chlorophyll detection in coastal waters based on environmental variables. Chlorophyll concentration is the most widely used measurement derived from ocean colour remote sensing data. In order to retrieve chlorophyll concentrations in highly turbid waters (case 2 waters) in the Pearl River estuary, a predictive model is...

Gubler, D.J., 2011: Emerging vector-borne flavivirus diseases: are vaccines the solution?. Expert Review of Vaccines 10(5): 563-565

Queisser, W.; Neu, G., 1970: Proliferation of an unspecific esterase positive (monocytopoietic) cell system in human bone marrow as observed from nucleic acid synthesis. Klinische Wochenschrift 48(20): 1229-1233

Jin,J.; Zimmerman,A.R., 2010: Abiotic interactions of natural dissolved organic matter and carbonate aquifer rock. Abiotic interactions between natural dissolved organic matter (NDOM) and carbonate aquifer rock may be controlling factors of biogeochemical processes and contaminant fate in carbonate aquifer systems. The importance and effects of these interacti...

Fleming, J.; Mathiesen, L., 1985: Final drive gear failure at Millaquin Mill. Proceedings of the Conference of the Australian Society of Sugar Cane Technologists: 21-325

Kampmann, T.; Kollner, V., 1994: Vergleichende Untersuchungen von Milbenpopulationen in Ackerboden bei unterschiedlicher Bewirtschaftungsintensitat. Mitteilungen aus der Biologischen Bundesanstalt fuer Land- und Forstwirtschaft Berlin-Dahlem, 295: 274-317

Yonezawa M.; Eguchi K.; Nagata H.; Kudo T.; Sekiba K., 1980: Metabolic regulation of amino acids during fetal development in rat liver. Acta Obstetrica et Gynaecologica Japonica (Japanese Edition) 32(12): 2167

Flott, S.; Rouse, J., 2005: Slx4 becomes phosphorylated after DNA damage in a Mec1/Tel1-dependent manner and is required for repair of DNA alkylation damage. Members of the RecQ family of DNA helicases, mutated in several syndromes associated with cancer predisposition, are key regulators of genome stability. The Saccharomyces cerevisiae SLX4 gene is required for cell viability in the absence of Sgs1,...

Westphal, J.F.; Brogard, J.M., 1997: Drug administration in chronic liver disease. Cirrhosis encompasses a range of pathophysiological changes that may alter drug disposition. Drugs that are dependent primarily on the liver for their systemic clearance are more likely to be subject to reduced elimination and subsequent accumulat...