+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Reduction in the mRNA expression of sVEGFR1 and sVEGFR2 is associated with the selection of dominant follicle in cows

, : Reduction in the mRNA expression of sVEGFR1 and sVEGFR2 is associated with the selection of dominant follicle in cows. Reproduction in Domestic Animals 51(6): 985-991

NlmCategory="UNASSIGNED">The vascular endothelial growth factor (VEGF) is essential for follicular development by promoting follicular angiogenesis, as well as for the proliferation and survival of granulosa cells. The biological effects of VEGF are regulated by two membrane receptors, VEGFR1 and VEGFR2, and two soluble receptors, sVEGFR1 and sVEGFR2, which play an antagonistic role. Thus, the objective of this study was to identify the mRNA expression pattern of total VEGF, VEGFR1, VEGFR2, sVEGFR1 and sVEGFR2 in bovine preselected follicles (PRF) and post-selected follicles (POF). The mRNA expression of these five genes in both granulosa cells (GC) and theca cells (TC) was compared between follicles classified as PRF and POF based on their diameter and on their ratio of estradiol/progesterone (E2/P4). Results showed a lower expression of mRNA of sVEGFR1 and sVEGFR2 in POF than in PRF (p < .05). Regarding the mRNA expression of total VEGF, VEGFR1 and VEGFR2, there was no difference between POF and PRF follicles (p > .05). Our results showed that the mRNA expression of VEGFR2 and sVEGFR1 was more abundant than the expression of VEGFR1 and sVEGFR2, while GC was the main source of mRNA for total VEGF. On the other hand, TC was the follicular compartment where the receptors were most expressed. Our results suggest that non-dominant follicles maintain a greater concentration of the mRNA expression of both membrane and soluble VEGF receptors. On the other hand, follicular dominance is related to a reduction in the mRNA expression of sVEGFR1 and sVEGFR2, which may favour VEGF binding with VEGFR2 and, hence, improve the follicular health and development.


PMID: 27650571

DOI: 10.1111/rda.12777

Other references

Buchanan, A.; Remaster, R., 1988: Absconders from a rural health centre in Papua New Guinea. The characteristics of patients absconding from a rural health centre in Papua New Guinea were compared with those of controls matched for distance of their residence from the health centre and for age, sex and diagnosis. It was found that abscond...

Garrity, W.F., 2011: Libraries and electronic health records: focus on "evidence" as part of treatment and care, not on "the library". Journal of the Medical Library Association 98(3): 210-211

Ebert, C.; Ebert, G.; Knipp, H., 1977: On the introduction of disulfide crosslinks into fibrous proteins and bovine serum albumin. Hydroxyl groups in serine side chains of collagen, silk fibroin, and bovine serum albumin (BSA) were converted to SH by tosylation. In collagen film, 50% of the serine OH groups could be thiolated at most. In fibroin, only 13% because of its compa...

Halstead, S.B.; Deen, J., 2002: The future of dengue vaccines. The health effects of air pollution have been subject to intense study in recent years. Exposure to pollutants such as airborne particulate matter and ozone has been associated with increases in mortality and hospital admissions due to respiratory...

Terenzi, H.F., 1988: Mating type response in Neurospora crassa. Early and transient changes in the patterns of protein synthesis in sexually stimulated mycelia. 1. Pulse labeling with [35S]-methionine, one-dimensional SDS-polyacrylamide gel electrophoresis and fluorography were used to study the pattern of protein synthesis in Neurospora crassa mycelia undergoing sexual development. 2. Contact of sexually...

Kives, S.L.; Levy, B.S.; Levine, R.L., 2003: Laparoscopic-assisted vaginal hysterectomy: American Association of Gynecologic Laparoscopists' 2000 membership survey. Journal of the American Association of Gynecologic Laparoscopists 10(2): 135-138

Mizunami, M.; Yamagata, N.; Nishino, H., 2010: Alarm pheromone processing in the ant brain: an evolutionary perspective. Social insects exhibit sophisticated communication by means of pheromones, one example of which is the use of alarm pheromones to alert nestmates for colony defense. We review recent advances in the understanding of the processing of alarm pheromo...

Cernenko, S.F.; Cernenko, E.S., 1955: Methods of producing hybrids between apple and pear.. An account of wide crossing at Micurinsk, dealing mainly with the methods employed to improve crossability, is presented. It includes general descriptions of the morphological characteristics of hybrids from direct and reciprocal crosses and notes...

Milici Robert C.; Blake Bascombe M.J.; Nolde Jack E.; Spears David; Sites Roy S.; Campbell Elizabeth V.M.; Denning Ryan; Freeman Phillip A., 1998: Use of GIS to study the coal resources of the Pocahontas No. 3 coal bed (Lower Pennsylvanian), Virginia and West Virginia, for the U. S. Geological Survey's national coal resources assessment. Abstracts with Programs - Geological Society of America 30(4): 51

M.M.teo-March; M.Z.bala; J.J.G.nzález-Badillo, 2012: Effects of the orientation of the maximum torque point with a Q-Ring non-circular chainring system on the BMX cycling sprint performance. The aim of this study was to observe the differences between the Q-Ring non-circular chainring system optimum chainring position (OCP) torque settings on the initial sprint of the BMX race. <section-title>Synthesis of the facts</section-t...