geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Reproducibility of compartmental subchondral bone morphometry in the mouse tibiofemoral joint


, : Reproducibility of compartmental subchondral bone morphometry in the mouse tibiofemoral joint. Bone 81(): 649-653

Evidence suggests that subchondral bone can be used as a predictor for the onset of osteoarthritis. As such, there is a need to accurately and reproducibly quantify subchondral bone in areas where osteoarthritis develops. In this paper, we present a novel technique for the segmentation of subchondral bone in the tibiofemoral joint and assess the reproducibility of this method with multiple measures and users. The right hind leg of seven C57BL/6 mice were excised and imaged in μCT. The menisci and patella were manually segmented and the image data was Gaussian filtered and binarized. An in-house algorithm was used to generate cortical and epiphyseal volumes of interest and standard morphometric indices for bone were computed. The intraclass correlation coefficient (ICC), absolute precision error (PE(SD)), and precision error as a percentage of the coefficient of variation of the repeated measurements (PE(%CV)) were calculated for each index. Additionally, an inter-user study was performed using the same indices and statistics. For repeated measures, ICC ranged from 0.869 (cortical bone volume fraction, femur) to 0.994 (degree of anisotropy, femur). Similarly, PE(%CV) ranged from 0.84% (cortical bone volume fraction, femur) to 5.11% (connectivity density, tibia). For repeated users, no effect was seen in the femur with a slight effect in the tibia. A novel method for the automatic segmentation of cortical and epiphyseal bone is presented and is shown to be reproducible in C57BL/6 mice. This tool will allow for high-throughput studies of osteoarthritis in animal models.

US$19.90

PMID: 26424216

DOI: 10.1016/j.bone.2015.09.014


Other references

Matsuno, T.; Ookubo, M.; Nishizawa, T.; Shimizu, I., 1984: Carotenoids of sea squirts 1. new marine carotenoids halocynthiaxanthin and mytiloxanthinone from halocynthia roretzi. New marine carotenoids, halocynthiaxathin (4) and mytiloxanthinone (5), were isolated from the sea squirt H. roretzi (Maboya in Japanese). The structures of 4 and 5 were established to be (3S,5R,6S,3'R)-5,6-epoxy-3,3'-dihydroxy-7',8...

Evans, P.D.; Talamo, B.R.; Kravitz, E.A., 1975: Octopamine neurons: morphology, release of octopamine and possible physiological role. Brain Research 90(2): 340-347

Satli, M.A., 1979: New data on serotoninergic mechanisms in ovulation in the cyclic female rat. Injection of parachlorophenylalanine on dioestrus II at 18:00 was shown to decrease ovulation frequency in 4-day cyclic female rats. This effect was overcome by either HTP, or oestradiol benzoate, when administered on dioestrus II, at 18:00 and 10...

Budhathoki, C.B.; Lynch, T.B.; Guldin, J.M., 2006: Individual tree growth models for natural even-aged shortleaf pine. Shortleaf pine (Pinus echinata Mill.) measurements were available from permanent plots established in even-aged stands of the Ouachita Mountains for studying growth. Annual basal area growth was modeled with a least-squares nonlinear regression me...

Görtz, D.Patrik.; Commons, M.Lamport., 2016: The stage-value model: Implications for the changing standards of care. The standard of care is a legal and professional notion against which doctors and other medical personnel are held liable. The standard of care changes as new scientific findings and technological innovations within medicine, pharmacology, nursing...

Kendis, J.B., 1964: Principles Of Comprehensive Therapy In Management Of The Alcoholic. Wisconsin Medical Journal 63: 188-192

Wang, Y.; Crawford, D.R.; Davies, K.J., 1996: adapt33, a novel oxidant-inducible RNA from hamster HA-1 cells. We have recently chosen to undertake a comprehensive evaluation of the modulation of gene expression by oxidative stress, using mRNA as a marker. Our model system is HA-1 hamster fibroblasts, using conditions under which we observe an adaptive res...

Buckett, K.J.; Peters, M.; Benjamin, P.R., 1990: Excitation and inhibition of the heart of the snail, Lymnaea, by non-FMRFamidergic motoneurons. 1. The present paper extends the model of neuronal control of the Lymnaea heart by the use of intracellular recording techniques to identify further types of cardioactive neurons in the CNS that, like the previously described E heart excitor (Ehe)...

Genda, H.; Okayama, H., 1978: Simulator for remote sensing and its application to soil moisture measurements. It is of great significance to experiment with a simulator for remote sensing to confirm the properties and meaning of remote-sensed information and to forecast certain phenomena. This paper describes a simulator for remote sensing. The simulator,...

Adedokun H.P.A., 1985: Modified voiding cystography in intersexuality report of the procedure in a case of intersex. Nigerian Journal of Paediatrics 12(1): 21-24