geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

SHCBP1 is required for midbody organization and cytokinesis completion


, : SHCBP1 is required for midbody organization and cytokinesis completion. Cell Cycle 13(17): 2744-2751

The centralspindlin complex, which is composed of MKLP1 and MgcRacGAP, is one of the crucial factors involved in cytokinesis initiation. Centralspindlin is localized at the middle of the central spindle during anaphase and then concentrates at the midbody to control abscission. A number of proteins that associate with centralspindlin have been identified. These associating factors regulate furrowing and abscission in coordination with centralspindlin. A recent study identified a novel centralspindlin partner, called Nessun Dorma, which is essential for germ cell cytokinesis in Drosophila melanogaster. SHCBP1 is a human ortholog of Nessun Dorma that associates with human centralspindlin. In this report, we analyzed the interaction of SHCBP1 with centralspindlin in detail and determined the regions that are required for the interaction. In addition, we demonstrate that the central region is necessary for the SHCBP1 dimerization. Both MgcRacGAP and MKLP1 are degraded once cells exit mitosis. Similarly, endogenous and exogenous SHCBP1 were degraded with mitosis progression. Interestingly, SHCBP1 expression was significantly reduced in the absence of centralspindlin, whereas centralspindlin expression was not affected by SHCBP1 knockdown. Finally, we demonstrate that SHCBP1 depletion promotes midbody structure disruption and inhibits abscission, a final stage of cytokinesis. Our study gives novel insight into the role of SHCBP in cytokinesis completion.

US$19.90

PMID: 25486361

DOI: 10.4161/15384101.2015.945840


Other references

Chairez, R.; Lister, R.M., 1973: A comparison of two strains of apple chlorotic leaf spot virus. An isolate of the virus from apple (A) and another from peach (P) were distinguished as distinct strs. on the basis of different symptoms on woody indicators and in severity of symptoms on Chenopodium quinoa. In serological ring precipitin tests A...

Casciari, R.J.; Berman, J.J.; Glauser, F.L., 1977: Acute febrile illness associated with bilateral pulmonary infiltrates after irradiation in a patient with Hodgkin's disease. An 18-year-old woman with stage IIIB Hodgkin's disease presented with minimal shortness of breath and progressed to fatal pulmonary insufficiency in five days. Biopsy and necropsy lung tissue specimens established the diagnosis of acute radia...

Mathew, A.; Brahmbhatt, B.; Rajesh, R.; Kurian, G.; Unni, V.N., 2010: Page kidney. Indian Journal of Nephrology 19(4): 170-171

Liu, Z.; Shen, Y.; Ott, J., 2011: Multilocus association mapping using generalized ridge logistic regression. In genome-wide association studies, it is widely accepted that multilocus methods are more powerful than testing single-nucleotide polymorphisms (SNPs) one at a time. Among statistical approaches considering many predictors simultaneously, scan st...

Roohafza, H.; Sarrafzadegan, N.; Sadeghi, M.; Rafieian-Kopaei, M.; Sajjadi, F.; Khosravi-Boroujeni, H., 2013: The association between stress levels and food consumption among Iranian population. Stress has been considered as a highly common disorder that has a complicated relation with dietary intake and has been linked with both increased and decreased dietary intake. This study was conducted to assess the association between food consum...

Bioulac-Sage, P.; Balabaud, C.; Zucman-Rossi, J., 2011: Will the pathomolecular classification of hepatocellular adenomas improve their clinical management?. Journal of Hepatology 55(1): 8-10

Dellinger, J.A., 1985: Monitoring the chronic effects of anticholinesterase pesticides in aerial applicators. Four pilots were monitored over a six month period for physiological signs of anticholinesterase exposures in this preliminary study. Two pilots were controls and two were aerial applicators whose spraying activities included the application of et...

Nakayama, K.; Ruth, J.L.; Cheng, Y.C., 1982: Differential effect of nucleoside analog triphosphates on ribonucleotide reductases from uninfected and herpes simplex virus-infected HeLa cells. Effects of the triphosphates of eight pyrimidine nucleoside analogs (5-substituted, 2'-fluoroara-, and acyclonucleosides) and acycloguanosine were examined on the ribonucleotide reductases prepared from uninfected and herpes simplex virus (ty...

Quaroni, S.; Rigamonti, G.; Saracchi, M.; Spreafico, M., 1986: Investigation on grapevine microflora i. epicuticular modifications caused by saprophitic fungi. The mycoflora associated with the grapevine cv. Barbera carposphere at the harvest is investigated. The fungal species most frequently isolated are Aureobasidium pullulans, Cladosporium herbarum and Penicillium thomii. The growth of three strains...

Dolan, B., 1998: The hospital hoppers. Nursing Times 94(30): 26-27