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Sequencing and analysis of globally obtained human respiratory syncytial virus A and B genomes

, : Sequencing and analysis of globally obtained human respiratory syncytial virus A and B genomes. Plos One 10(3): E0120098-E0120098

Human respiratory syncytial virus (RSV) is the leading cause of respiratory tract infections in children globally, with nearly all children experiencing at least one infection by the age of two. Partial sequencing of the attachment glycoprotein gene is conducted routinely for genotyping, but relatively few whole genome sequences are available for RSV. The goal of our study was to sequence the genomes of RSV strains collected from multiple countries to further understand the global diversity of RSV at a whole-genome level. We collected RSV samples and isolates from Mexico, Argentina, Belgium, Italy, Germany, Australia, South Africa, and the USA from the years 1998-2010. Both Sanger and next-generation sequencing with the Illumina and 454 platforms were used to sequence the whole genomes of RSV A and B. Phylogenetic analyses were performed using the Bayesian and maximum likelihood methods of phylogenetic inference. We sequenced the genomes of 34 RSVA and 23 RSVB viruses. Phylogenetic analysis showed that the RSVA genome evolves at an estimated rate of 6.72 × 10(-4) substitutions/site/year (95% HPD 5.61 × 10(-4) to 7.6 × 10(-4)) and for RSVB the evolutionary rate was 7.69 × 10(-4) substitutions/site/year (95% HPD 6.81 × 10(-4) to 8.62 × 10(-4)). We found multiple clades co-circulating globally for both RSV A and B. The predominant clades were GA2 and GA5 for RSVA and BA for RSVB. Our analyses showed that RSV circulates on a global scale with the same predominant clades of viruses being found in countries around the world. However, the distribution of clades can change rapidly as new strains emerge. We did not observe a strong spatial structure in our trees, with the same three main clades of RSV co-circulating globally, suggesting that the evolution of RSV is not strongly regionalized.


PMID: 25793751

DOI: 10.1371/journal.pone.0120098

Other references

Weiss, Mj, 1984: The sugarbeet root maggot. Montguide MT: Agriculture Montana State University Cooperative Extension Service: 84 (8406)

Landis, B.; Jovanovic, L.; Landis, E.; Peterson, C.M.; Groshen, S.; Johnson, K.; Miller, N.E., 1985: Effect of stress reduction on daily glucose range in previously stabilized insulin-dependent diabetic patients. Diabetes Care 8(6): 624-626

Vivori, E.; Bush, G.H., 1977: Modern aspects of the management of the newborn undergoing operation. British Journal of Anaesthesia 49(1): 51-57

Rubin J.A., 1985: Mortality and avoidance of competitive overgrowth in encrusting bryozoa. Forty-five species of sessile animals, including 32 spp. cyclostome and cheilostome bryozoans, settled on experimental panels over a 1 yr period. Colonies were readily overgrown by tubes of the serpulid Pomatoceros triqueter (L.) which became the...

Elsasser, T.H.; Rosebrough, R.W.; Rumsey, T.S.; Moseley, W.M., 1996: Hormonal and nutritional modulation of hepatic arginase activity in growing cattle. The hormonal and nutritional modulation of hepatic arginase activity (HARG) was characterized in growing cattle in two studies. In the first study, 20 steers (initial weight, 182 +- 2 kg) were assigned in equal numbers to either Synovex-S (SYN) (e...

Fuchs, I.; Tarabin, S.; Kafka, M., 2015: Relapsing Fever: Diagnosis Thanks to a Vigilant Hematology Laboratory. Three cases of relapsing fever from southern Israel were diagnosed promptly thanks to vigilance of the hematology laboratory technicians. In this region of Israel, patients presenting with prolonged fever and leukopenia without localizing symptoms...

Karlsson, E.A.; Liedberg, G.M.; Sandqvist, J.L., 2017: Initial evaluation of psychometric properties of a structured work task application for the Assessment of Work Performance in a constructed environment. The Swedish Social Insurance Administration has developed a new assessment tool for sickness insurance. This study is a part of the initial evaluation of the application, called the Assessment of Work Performance, Structured Activities, and focuse...

Guzman Flores, C.; M.J.N.grete; Guardiola, A.F.rnandez; Del Pozo, E.C., 1954: Spinal shock in kittens. Kittens and adult cats were subjected to complete medullary section following which a mechanical stimulus applied to one of the posterior extremities. In most kittens the crossed extensor reflex activity of the cord was markedly increased immediat...

Serkina, A.V., 1964: Experiences With Screening Examinations For The Detection And Treatment Of Patients In The Initial Phases Of Obliterative Endarteritis In The Irkutsk Region. Klinicheskaia Meditsina 42: 140-142

Kitchell, J.F.; Breck, J.E., 1980: Bioenergetics model and foraging hypothesis for sea lamprey (Petromyzon marinus). A bioenergetics model for the growth of lamprey (Petromyzon marinus) in their parasitic stage was used to estimate feed intake of lamprey and their impact on host fish. Results were applied to case histories from the Great Lakes of north America.