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Serum from Nipah virus patients recognises recombinant viral proteins produced in Escherichia coli

, : Serum from Nipah virus patients recognises recombinant viral proteins produced in Escherichia coli. Japanese Journal of Infectious Diseases (): -

NlmCategory="UNASSIGNED">The genes for Nipah virus (NiV) N, F and G proteins were amplified from viral RNA, cloned into the plasmid pTriEx-3 Hygro, expressed and purified using immobilized metal affinity chromatography. The recombinant NiV proteins, rNiV-N, rNiV-F and rNiV-G, were successfully expressed in E. coli and purified with a yield of 4mg/L, 16mg/L and 4mg/L, respectively. All three recombinant viral proteins reacted with all 19 NiV-positive human sera with rNiV-N and rNiV-G being more immunogenic. The recombinant viral proteins did not react with any of the 12 NiV-negative sera. Serum from a case of late-onset relapsing NiV infection complication, however, was found to be primarily reactive to rNiV-G only. Additionally, there is a distinctive variation in the profile of antigen-reactive bands between the sample from a case of relapsing NiV encephalitis and that of acute NiV infection. Overall findings suggest that the recombinant viral proteins have the potential to be developed further for use in the detection of NiV infection, and as an important tool for continuous biosurveillance of NiV infection in resource limited settings.


PMID: 27169942

DOI: 10.7883/yoken.JJID.2015.501

Other references

Hou, G.; Buxton, J.W.; Switzer, D., 1992: Evaluation of methods to prevent root growth into capillary mats. Hortscience 27(11): 1175

Blaas, H.G.; Eik-Nes, S.H., 1998: Advances in the imaging of the embryonic brain. Aim. Description of the development of the human embryonic brain visualized by transvaginal ultrasound. Methods. In two studies, healthy pregnant women without any pregnancy complications and normal outcomes were examined by transvaginal ultrasoun...

Belanger, J., 1977: A sense of humus it takes both NPK and BYM. Countryside 61 (3) 24-26

Crescenzo, R.; Bianco, F.; Mazzoli, A.; Giacco, A.; Liverini, G.; Iossa, S., 2016: Skeletal muscle mitochondrial energetic efficiency and aging. Aging is associated with a progressive loss of maximal cell functionality, and mitochondria are considered a key factor in aging process, since they determine the ATP availability in the cells. Mitochondrial performance during aging in skeletal mu...

Pauchard, B.; Higashigaito, K.; Lamri-Senouci, A.; Knebel, J-Francois.; Berthold, D.; Verdun, F.Robert.; Alkadhi, H.; Schmidt, S., 2017: Iterative Reconstructions in Reduced-Dose CT: Which Type Ensures Diagnostic Image Quality in Young Oncology Patients?. To compare adaptive statistical iterative reconstruction (ASIR) and model-based iterative reconstruction (MBIR) algorithms for reduced-dose computed tomography (CT). Forty-four young oncology patients (mean age 30 ± 9 years) were included. Af...

Fang, F.G.; Fields, H.L.; Lee, N.M., 1986: Action at the mu receptor is sufficient to explain the supraspinal analgesic effect of opiates. Mu, delta and kappa opiate receptors have been implicated in the production of analgesia. In order to study the relative contributions of these receptor types to supraspinally mediated analgesia, apparent pA2 values and rank order potencies were d...

Letti, Nicanor, 1958: Hypo-physeal region of the turtle Phrynops hilarii. Folia Clin Et Biol 28: 164-167, -59

Anonymous, 1964: International Colloquium on Medical Mycology, Antwerp, Belgium, 6-8 December, 1963. The symposium is divided into 3 section topics: 6 papers on Cryptococci with the presidents A. Dubois and H. Gotz presiding; 13 papers on Dermatophytes with the president J. Gentles presiding; 4 papers on Hlstopathologla with the president E. Drou...

Jerina D.M., 1973: The biological formation and disposition of arene oxides. Lloydia (Cincinnati) 36(4): 432

Skinhoj, E.; Olesen, J.; Paulson, O.B., 1973: Influence of smoking and nicotine on cerebral blood flow and metabolic rate of oxygen in man. Journal of Applied Physiology 35(6): 820-822