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Serum from Nipah virus patients recognises recombinant viral proteins produced in Escherichia coli


, : Serum from Nipah virus patients recognises recombinant viral proteins produced in Escherichia coli. Japanese Journal of Infectious Diseases (): -

NlmCategory="UNASSIGNED">The genes for Nipah virus (NiV) N, F and G proteins were amplified from viral RNA, cloned into the plasmid pTriEx-3 Hygro, expressed and purified using immobilized metal affinity chromatography. The recombinant NiV proteins, rNiV-N, rNiV-F and rNiV-G, were successfully expressed in E. coli and purified with a yield of 4mg/L, 16mg/L and 4mg/L, respectively. All three recombinant viral proteins reacted with all 19 NiV-positive human sera with rNiV-N and rNiV-G being more immunogenic. The recombinant viral proteins did not react with any of the 12 NiV-negative sera. Serum from a case of late-onset relapsing NiV infection complication, however, was found to be primarily reactive to rNiV-G only. Additionally, there is a distinctive variation in the profile of antigen-reactive bands between the sample from a case of relapsing NiV encephalitis and that of acute NiV infection. Overall findings suggest that the recombinant viral proteins have the potential to be developed further for use in the detection of NiV infection, and as an important tool for continuous biosurveillance of NiV infection in resource limited settings.

US$19.90

PMID: 27169942

DOI: 10.7883/yoken.JJID.2015.501


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