geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Structural basis for recognition of G-1-containing tRNA by histidyl-tRNA synthetase


, : Structural basis for recognition of G-1-containing tRNA by histidyl-tRNA synthetase. Nucleic Acids Research 43(5): 2980-2990

Aminoacyl-tRNA synthetases (aaRSs) play a crucial role in protein translation by linking tRNAs with cognate amino acids. Among all the tRNAs, only tRNA(His) bears a guanine base at position -1 (G-1), and it serves as a major recognition element for histidyl-tRNA synthetase (HisRS). Despite strong interests in the histidylation mechanism, the tRNA recognition and aminoacylation details are not fully understood. We herein present the 2.55 Å crystal structure of HisRS complexed with tRNA(His), which reveals that G-1 recognition is principally nonspecific interactions on this base and is made possible by an enlarged binding pocket consisting of conserved glycines. The anticodon triplet makes additional specific contacts with the enzyme but the rest of the loop is flexible. Based on the crystallographic and biochemical studies, we inferred that the uniqueness of histidylation system originates from the enlarged binding pocket (for the extra base G-1) on HisRS absent in other aaRSs, and this structural complementarity between the 5' extremity of tRNA and enzyme is probably a result of coevolution of both.

US$19.90

PMID: 25722375

DOI: 10.1093/nar/gkv129


Other references

L.Bianco, S., 1951: Transposition of the great vessels of the heart. Il Lattante 22(9): 513-535

Santos, J.L.; Cabranes, J.A.; Almoguera, I.; Ramos, J.A.; Vazquez, C.; Angeles, F., 1989: Clinical implications of determination of plasma haloperidol levels. The aim of this study was to analyze the clinical utility of monitoring plasma levels, since the utility of monitoring is not yet well established. After a washout period, 30 schizophrenic patients were given fixed doses of haloperidol for 3 weeks...

Young, R.H.; Scully, R.E., 1995: Non-neoplastic disorders of the ovary. Fox, H [Editor] Haines and Taylor obstetrical and gynaecological pathology, Fourth edition 699-726

Mann, F.D.; Steinke, T.D., 1993: Biological nitrogen fixation (acetylene reduction) associated with blue-green algal (cyanobacterial) communities in the Beachwood Mangrove Nature Reserve II. Seasonal variation in acetylene reduction activity. Seasonal variation in nitrogen fixation of blue-green algae associated with Avicennia marina pneumatophores and wet and dry surface sediments were investigated in the Beachwood Mangrove Nature Reserve, South Africa, by means of the acetylene reduc...

Pokrovskii V.V.; Servetskii K.L., 1988: Transfer of human immunodeficiency virus hiv in breast feeding and heterosexual contacts. Two cases of the transfer of HIV infection are described: a child got HIV infection through breast feeding from the mother infected with HIV as the result of blood transfusion made 2 weeks after the birth of the child and 2 men were infected with...

Poelzl, G.; Ess, M.; Von der Heidt, A.; Rudnicki, M.; Frick, M.; Ulmer, H., 2013: Concomitant renal and hepatic dysfunctions in chronic heart failure: clinical implications and prognostic significance. The cardio-renal syndrome is common and eGFR is an established biomarker in chronic heart failure (CHF). Recent findings also indicate a predictive role of liver function abnormalities such as GGT in CHF. We aimed to jointly investigate the charac...

Gailonskaia, I.N.; Kopelev, M.F.; Busuek, G.P.; Kuznetsov, V.P.; Lozinskaia, T.M., 1973: The clinical course of influenza in patients treated with interferon and symptomatic agents. Klinicheskaia Meditsina 51(2): 117-120

Dyar Melinda Darby, 1985: Quenching effects on iron site partitioning in the Apollo 17 orange glass composition. NASA Technical Memorandum 87563: 223-224

Mosconi, S.; Streit, M.; Brönimann, M.; Braathen, L.R., 2002: Eosinophilic fasciitis (Shulman syndrome). We report a case of a 30-year-old Caucasian patient with progressive sclerosis of the skin mainly on the upper limbs which was diagnosed as eosinophilic fasciitis (Shulman syndrome). Circulating antibodies against Borrelia burgdorferi were detecte...

Tanaka, K.; Miyata, M.; Ueda, M.; Yazaki, T.; Tomioka, H.; Mukai, H.; Yasuda, K.; Nakajima, M., 1997: Immunohistochemical evaluation of mucin producing tumor of the pancreas. Gastroenterology 112(4 SUPPL ): A486