geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Test of Cure for Anogenital Gonorrhoea Using Modern RNA-Based and DNA-Based Nucleic Acid Amplification Tests: A Prospective Cohort Study


, : Test of Cure for Anogenital Gonorrhoea Using Modern RNA-Based and DNA-Based Nucleic Acid Amplification Tests: A Prospective Cohort Study. Clinical Infectious Diseases 62(11): 1348-1355

The use of nucleic acid amplification tests (NAATs) to diagnose Neisseria gonorrhoeae infections complicates the performance of a test of cure (TOC) to monitor treatment failure, if this is indicated. As evidence for the timing of TOC using modern NAATs is limited, we performed a prospective cohort study to assess time to clearance when using modern RNA- and DNA-based NAATs. We included patients with anogenital gonorrhoea visiting the Sexually Transmitted Infection Clinic Amsterdam from March through October 2014. After treatment with ceftriaxone mono- or dual therapy (with azithromycin or doxycycline), anal, vaginal, or urine samples were self-collected during 28 consecutive days, and analyzed using an RNA-based NAAT (Aptima Combo 2) and a DNA-based NAAT (Cobas 4800). Clearance was defined as 3 consecutive negative results, and blips as isolated positive results following clearance. We included 77 patients; 5 self-cleared gonorrhoea before treatment and 10 were lost to follow-up. Clearance rate of the remaining 62 patients was 100%. Median time to clearance was 2 days, with a range of 1-7 days for RNA-based NAAT and 1-15 days for DNA-based NAAT. The risk of finding a blip after clearance was 0.8% and 1.5%, respectively. One patient had a reinfection. If indicated, we recommend that TOC be performed for anogenital gonorrhoea at least 7 or 14 days after administering therapy, when using modern RNA- or DNA-based NAATs, respectively. When interpreting TOC results for possible treatment failure, both the occurrence of blips and a possible reinfection need to be taken into account.

US$19.90

PMID: 26962074

DOI: 10.1093/cid/ciw141


Other references

Liu Lunhua, 1994: Environmental geological problems of the development and utilization of water and soil resources in Ganzhou, Jiangxi. This paper systematically describes the crust, ground foundation, stability of the river bank, submersion of the urban area, silt sedimentation of the river course and descent of the underground water level as well as the water and soil pollution...

Sokoloff, 1995: Theory of the contribution to sliding friction from electronic excitations in the microbalance experiment. Physical Review. B, Condensed Matter 52(7): 5318-5322

Pancham, K.; Sami, I.; Perez, G.F.; Huseni, S.; Kurdi, B.; Rose, M.C.; Rodriguez-Martinez, C.E.; Nino, G., 2016: Human Metapneumovirus Infection is Associated with Severe Respiratory Disease in Preschool Children with History of Prematurity. Human metapneumovirus (HMPV) is a recently discovered respiratory pathogen of the family Paramyxoviridae, the same family as that of respiratory syncytial virus (RSV). Premature children are at high risk of severe RSV infections, however, it is un...

Tan, B.; Joseph, L.; Deng, W.; Liu, L.L., Q.; Cline, K.M.carty, D., 2003: Molecular characterization of the Arabidopsis 9-cis epoxycarotenoid dioxygenase gene family. A key regulated step in abscisic acid (ABA) biosynthesis in plants is catalyzed by 9-cis epoxycarotenoid dioxygenase (NCED), which cleaves 9-cis xanthophylls to xanthoxin, a precursor of ABA. In Arabidopsis, ABA biosynthesis is controlled by a sma...

Bonaventure, N.; Wioland, N.; Jardon, B., 1985: On GABAergic mechanisms in the optokinetic nystagmus of the frog: effects of bicuculline, allylglycine and SR 95103, a new GABA antagonist. In a monocular situation, an intravitreal injection of the GABA antagonists, bicuculline or SR 95103 provoked both the suppression of the optokinetic nystagmus (OKN) related to the injected eye and the appearance of a Nasal-Temporal (N-T) componen...

Grassi, G., 2013: Renal denervation in cardiometabolic disease: concepts, achievements and perspectives. Growing evidence suggests that renal denervation may reduce elevated blood pressure values in resistant hypertension. More recently a number of studies have assessed the effects of the intervention on the dysmetabolic disarray (i.e. insulin resist...

Hamlin B.G., 1968: The genus carex m sect echinocholaenae in new zealand typification classification and descriptions of new species carex astonii m new species carex druceana m new species carex fretalis m new species carex tahoata m new species 2 new combinations. Records of the Dominion Museum (Wellington) 6(7): 97-111

Anonymous, 1973: Annual report of the National Institute of Genetics, No. 23, 1972. Papers abstracted appear in the appropriate sections of ABA.

Theodossiadis, G.P., 1985: Pupilloplasty in aphakic and pseudophakic pupillary block glaucoma. Pupilloplasty has given positive results in 40 out of 45 cases of aphakic and pseudoaphakic secondary block glaucoma after one or two laser sessions. Since we first applied this method we have arrived at the following conclusions: Pupilloplasty mu...

Sundaresh, H.N., 1974: Studies on integrated control of root-knot nematode Meloidogyne incognita Chitwood. In trials with tomatoes and capsicums grown in soil infested with M. incognita comparing the effects of crop rotation with or without nematicide treatment, the best results were obtained from alternate cropping with maize, supplemented by treatmen...