+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

The Effect of 1α,25(OH)2D3 on Osteogenic Differentiation of Stem Cells from Dental Pulp of Exfoliated Deciduous Teeth

, : The Effect of 1α,25(OH)2D3 on Osteogenic Differentiation of Stem Cells from Dental Pulp of Exfoliated Deciduous Teeth. Journal of Dentistry 17(4): 348-353

Stem cells from human exfoliated deciduous teeth (SHEDs) are a population of highly proliferative cells, being capable of differentiating into osteogenic, odontogenic, adipocytes, and neural cells. Vitamin D3 metabolites such as 1α, 25-dihydroxyvitamin D3 are key factors in the regulation of bone metabolism. The aim of this study was to investigate the effect of 1α, 25-dihydroxyvitamin D3 on osteogenic differentiation (alkaline phosphatase activity and alizarin red staining) of stem cells of exfoliated deciduous teeth. Dental pulp was removed from freshly extracted primary teeth and immersed in a digestive solution. Then, the dental pulp cells were immersed in α-MEM (minimum essential medium) to which 10% fetal bovine serum was added. After the third passage, the cells were isolated from the culture plate and were used for osteogenic differentiation. As a control group, the cells were cultured in osteogenic cell culture medium. As the case group, the cells were cultured in osteogenic culture medium supplemented with 100 nM 1α,25 (OH)2D3. The alkaline phosphatase (ALP) activity and alizarin red staining were analyzed to evaluate the osteogenic differentiation at day 21. The results were analyzed by using t-test. Compared with the control group, significant increase was observed in ALP activity of SHEDs after being treated with 1α,25(OH)2D3 (p= 0.002). Alizarin red staining demonstrated that the cells exposed to 1α,25(OH)2D3 induced higher mineralized nodules (p< 0.001). Osteoblast differentiation in SHEDs was stimulated by 1α,25(OH) 2D3. It can be concluded that 1α,25(OH)2D3 can improve osteoblastic differentiation.


PMID: 27942551

Other references

Doss, M.; Pinkston, K., 1993: Bagworm control on eastern red cedar, 1992. Burditt, A K , Jr [Editor] Insecticide and Acaricide Tests : 347

Gregoriades, A.; Guzman, G.G.; Paoletti, E., 1990: The phosphorylation of the integral membrane (M1) protein of influenza virus. The phosphorylation of the internal and integral membrane (M1) protein of influenza virus was studied. Four points can be made based on the data: (1) The M1 contains at least two moles of phosphate per mole of M1. (2) Phosphorylation of M1 is cons...

Sauerbrei, A.; Ulbricht, A.; Wutzler, P., 2003: Semi-quantitative detection of viral RNA in influenza A virus-infected mice for evaluation of antiviral compounds. The aim of the study was to establish a murine model for sensitive screening of potential compounds with in vitro anti-influenza A virus activity. The evaluation in this in vivo model is based on semi-quantitative detection of viral RNA using one-...

Takahashi, R.; Misawa, H.; Yokoji, H.; Hayashi, M.; Komori, T.; Inaba, A.; Ohtake, T.; Hu, J.; Deguchi, T., 1994: An RNA splicing mutation that disrupts the human ciliary neurotrophic factor gene. Society for Neuroscience Abstracts 20(1-2): 1311

Symeonidis A.; Zoumbos N., 1991: Defective autologous and allogeneic mixed lymphocyte reaction mlr in patients with primary myelodysplastic syndromes mds. Leukemia Research 15(SUPPL): 29

Kolata, G.B., 1980: Cryptography: a new clash between academic freedom and national security. Science 209(4460): 995-996

Atkinson, R.L.; Mcgarvey, D.J.; Mays, J.; Williams, B., 1998: Photochemical reactions of carbophenothion. Abstracts of Papers American Chemical Society 216(1-3): AGRO 96

Xu, J-r., 1986: Early Jurassic Qiyangia fauna from Daye, Hubei. Acta Palaeontologica Sinica, 254: 454-462

Klee, W.A.; Simonds, W.F.; Sweat, F.W.; Burke, T.R.; Jacobson, A.E.; Rice, K.C., 1982: Identification of a Mr 58 000 glycoprotein subunit of the opiate receptor. A Mr 58 000 subunit of the opiate receptor has been identified using tritiated fentanyl isothiocyanate, a potent opiate alkylating reagent with specificity for the delta-opiate receptor subclass. The subunit is alkylated in the presence of dextror...

Ayers Kerry L.; Brennand Tracy A., 2003: Glacigenic undulating terrain on the southeastern plains of Canada; evidence of glaciotectonism or subglacial meltwater erosion?. Previously glaciated regions of the Southeastern Plains of Canada exhibit tracts of undulating terrain. These sets of broad, subparallel ridges separated by depressions can be identified on digital elevation models and Landsat images of southern M...