+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

The effect of experimental osteoporosis on bone regeneration: part 2, proteomics results

, : The effect of experimental osteoporosis on bone regeneration: part 2, proteomics results. Clinical Oral Implants Research (): -

To identify and describe protein expression in a Wistar rat calvarial critical size defect (CSD) model following treatment with guided bone regeneration in healthy and osteoporotic conditions. Thirty-six 10-month-old female Wistar rats were used. Half of them were ovariectomized (OVX) and fed with a low-calcium diet to induce an osteoporotic-like status. In each animal of both groups, two 5-mm calvarial CSDs were treated with deproteinized bovine bone mineral graft particles and a bilayer collagen membrane. Six OVX and six control rats were randomly euthanized at 7, 14, and 30 days. One defect/animal was randomly chosen for proteomic analysis. Differently expressed proteins between the two groups were identified with matrix-assisted laser desorption time-of-flight mass spectrometry and liquid chromatography-mass spectrometry/mass spectrometry. At 7 days, 29 and 27 proteins were, respectively, identified in the healthy and OVX animals. At 14 days, 103 proteins were detected in the healthy controls and 20 proteins in the OVX rats, while at 30 days, 31 and 75 proteins were identified, respectively. Only limited proteins known to play a role in the later stages of bone formation and maturation were identified within the animals 'proteomes. The osseous formation process was quite immature even at 30 days of healing. An overexpression of inflammatory and stress response pathways was detected in the OVX animals, as well as a tendency toward a delayed maturation of the osseous wound and a reduced/delayed differentiation of osteoblast cell precursors.


PMID: 27580862

DOI: 10.1111/clr.12950

Other references

Nazir, F.S.; Muir, K.W., 2004: Prolonged interval between vertebral artery dissection and ischemic stroke. Neurology 62(9): 1646-1647

González-Granado, L.I., 2011: Common manifestation of common variable immunodeficiency and its diagnostic criteria. Indian Journal of Pathology & Microbiology 53(4): 893-893

Price, E.R.; Hargreaves, J.; Lipson, J.A.; Sickles, E.A.; Brenner, R.James.; Lindfors, K.K.; Joe, B.N.; Leung, J.W.T.; Feig, S.A.; Bassett, L.W.; Ojeda-Fournier, H.; Daniel, B.L.; Kurian, A.W.; Love, E.; Ryan, L.; Walgenbach, D.D.; Ikeda, D.M., 2014: The California breast density information group: a collaborative response to the issues of breast density, breast cancer risk, and breast density notification legislation. In anticipation of breast density notification legislation in the state of California, which would require notification of women with heterogeneously and extremely dense breast tissue, a working group of breast imagers and breast cancer risk speci...

Ikezaki, S.; Nishikawa, A.; Furukawa, F.; Tanakamura, Z.; Kim, H.C.; Mori, H.; Takahashi, M., 1996: Chemopreventive effects of 24R,25-dihydroxyvitamin D3, a vitamin D3 derivative, on glandular stomach carcinogenesis induced in rats by N-methyl-N'-nitro-N-nitrosoguanidine and sodium chloride. The modifying effects of 24R,25-dihydroxyvitamin D-3 (24R,25(OH)-2D-3), a vitamin D3 derivative, on glandular stomach carcinogenesis were investigated in male Wistar rats by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and sodium chloride expo...

Glaister, J.A., 1994: Projective drawings: helping adult survivors of childhood abuse recognize boundaries. 1. Boundary issues for adult childhood trauma survivors are complex, problematic, difficult to resolve, and occur on a level of unawareness. 2. Boundary concepts can be found in all types of projective drawings. 3. Projective drawings can facilita...

McCartney, G.; Hanlon, P., 2010: Oil, health, and healthcare. Ramifications of peak oil. Bmj 341: C5796-C5796

Davies, S.S.; Bodine, C.; Matafonova, E.; Pantazides, B.G.; Bernoud-Hubac, N.; Harrison, F.E.; Olson, S.J.; Montine, T.J.; Amarnath, V.; Roberts, L.Jackson., 2012: Treatment with a γ-ketoaldehyde scavenger prevents working memory deficits in hApoE4 mice. Both inflammation and oxidative injury are features of Alzheimer's disease (AD), but the contribution of these intertwined phenomena to the loss of working memory in this disease is unclear. We tested the hypothesis that highly reactive γ-ke...

Martin, A.R.; Pilar, G., 1964: Quantal Components Of The Synaptic Potential In The Ciliary Ganglion Of The Chick. Journal of Physiology 175: 1-16

Hagiya, K., 1952: Physiological studies on the manifestation of pithy tissue in root crops. II. On varietal differences in the characters concerned with the occurrence of pithy tissue in radishes.. Studies on varietal differences in pithiness in Japanese radishes are reported. Pithiness is associated with rapid root growth, an early decline in the leaf/root ratio, a lower starch content and less soluble matter. The distribution of pithy tiss...

Gadea, I.; Cuenca-Estrella, M.; Benito, N.; Blanco, A.; Fernández-Guerrero, M.L.; Valero-Guillén, P.L.; Soriano, F., 2000: Bordetella hinzii, a "new" opportunistic pathogen to think about. Journal of Infection 40(3): 298-299